C 5050 digital camera

Manufactured by Olympus

Sourced in Japan

The C-5050 digital camera is a high-quality imaging device designed for laboratory applications. It features a 5-megapixel sensor and can capture images with a resolution of up to 2560 x 1920 pixels. The camera is capable of recording video at 30 frames per second.

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Lab products found in correlation

Bx51 microscope, by Olympus (22 mentions) Image pro express 1, by Media Cybernetics (10 mentions) Histostain plus bulk kit, by Thermo Fisher Scientific (6 mentions) Normal goat serum (ngs), by Thermo Fisher Scientific (4 mentions) Bx51 light microscope, by Olympus (3 mentions) Mr 2145, by Leica camera (2 mentions) Rm2145, by Leica camera (2 mentions) Arc0102, by Thermo Fisher Scientific (2 mentions) Nbp1 51224, by Novus Biologicals (2 mentions) 859043 histostain plus kit, by Thermo Fisher Scientific (2 mentions) Nbp1 47581, by Novus Biologicals (2 mentions) Nb100 479, by Novus Biologicals (2 mentions) Axiostar plus light microscope, by Leica camera (1 mentions) Dm2500 compound microscope, by Leica camera (1 mentions) S 4700, by Hitachi (1 mentions) Histostain plus bulk kit, by Bioss Antibodies (1 mentions) Sc 651, by Santa Cruz Biotechnology (1 mentions) Dm4000 microscope, by Olympus (1 mentions) Dako pen, by Agilent Technologies (1 mentions) Mayer s hematoxylin, by Merck Group (1 mentions)

33 protocols using c 5050 digital camera

Quantitative Immunohistochemistry of Sciatic Nerve NGF

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Rats were perfused intracardially with 4 % formaldehyde for histology and quantita-tive immunohistochemistry. Briefl y, sciatic nerves were embedded in paraffi n, sectioned at 5 μm thickness via microtome (Leica RM 2145). All sections were photographed with Olympus C-5050 digital camera mounted on an Olympus BX51 microscope. Axonal thickness was measured using Image-Pro Express 1.4.5 (Media Cybernetics, Inc. USA).
Sections were treated with 10 % H 2 O 2 for 30 minutes to remove endogenous peroxidase activity before being blocked with 10 % normal goat serum (Invitrogen) for 1 hour at room temperature for immunohistochemical analysis. After that, slices were treated for 24 hours at 4 °C with primary antibodies against nerve growth factor (NGF) (Santacruz Biotechnology; 1/100). The Histostain-Plus Bulk kit (Invitrogen) was used to detect antibodies against rabbit IgG, and 3,3' diaminobenzidine (DAB) was utilized to view the fi nal product. All sections were cleaned in PBS before being inspected with an Olympus BX51 microscope and photographed with an Olympus C-5050 digital camera. Quantitative immunohistochemistry was performed on all groups and six slices from each animal. Under a light microscope at x100 magnifi cation, two blinded observers counted the total immune-positive Schwann cells. Data were expressed as mean ± SEM.

Gonullu E., Dagistan G., Erkin Y., Erdogan M.A, & Erbas O. (2023). Demonstration of the protective effect of propofol in rat model of cisplatin-induced neuropathy. Bratislavske lekarske listy, 124(1).

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Histopathological Evaluation of Rectal Tissue

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Formalin-fixed rectum sections in 4 μm thickness were stained with hematoxylin & eosin. All sections were photographed with Olympus C-5050 digital camera mounted on Olympus BX51 microscope.
Histopathological scores were assigned using the following criteria of MacPherson and Pfeiffer^{7 (link)}: 0, intact epithelium, no leukocytes or hemorrhage: 1, <25% disrupted epithelium, focal leukocyte infiltrates, and focal hemorrhage; 2, 25% disrupted epithelium, focal leukocyte infiltrates, and focal hemorrhage; 3, 50% disrupted epithelium, widespread leukocytes, and hemorrhage; 4, >50% disrupted epithelium, extensive leukocyte infiltration, and hemorrhage.

Ercan G., Yigitturk G, & Erbas O. (2020). Therapeutic effect of adenosine on experimentally induced acute ulcerative colitis model in rats. Acta Cirúrgica Brasileira, 34(12), e201901204.

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Ostracod Diversity and Identification in Lake Baikal

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Samples were taken from 11–15 m depths by SCUBA diving from the shore of Lake Baikal. Three bottom types were sampled: rock, mud, and sand. Ostracods were sorted alive on the spot and immediately fixed in 97% ethyl alcohol. Dissection and identification was done with the aid of Zeiss Axiostar-plus light microscope and Leica DM 2500 compound microscope, equipped with N-Plan objectives, respectively. Scanning Electron Microscope (SEM) photographs were taken with a Hitachi S-4700 at Eulji University (Seoul). Photographs of Zenker organ and hemipenis were taken with Olympus C-5050 digital camera mounted on Olympus PX51 compound microscope.
Collected ostracods were identified with the aid of Mazepova (1990) . The terminology for A1, Md, Mxl, L5 and L6 follows Broodbakker and Danielopol (1982) , and for L7 Meisch (1996) . Here, the view of Meisch (2007) regarding the terminology and homology of the most posterior appendage on the ostracod body (“furca”) is accepted.

Karanovic I, & Sitnikova T.Y. (2017). Morphological and molecular diversity of Lake Baikal candonid ostracods, with description of a new genus. ZooKeys.

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Quantification of α-Synuclein Immunostaining

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40 μm thick cross sections were taken with a microtome (Leica MR 2145) from paraformaldehyde-fixed and paraffin-embedded cerebellum tissue. The sections were incubated with H₂O₂ (10%) for 30 min to eliminate endogenous peroxidase activity and blocked with 10% normal goat serum (Invitrogen) for 1 hour at room temperature. Subsequently, sections were incubated in primary antibodies (α-synuclein, Bioss Inc.; 1/100) for 24 h at 4°C. Antibody detection was performed with the Histostain-Plus Bulk kit (Invitrogen) against rabbit IgG, and 3,3′-diaminobenzidine (DAB) was used to visualize the final product. All sections were washed in PBS and photographed with an Olympus C-5050 digital camera mounted on an Olympus BX51 microscope. Brown cytoplasmic stained cells were scored as positive for α-synuclein immunostaining. The number of α-synuclein (+) cells was assessed by systematically scoring at least 100 Purkinje cells per field in 10 fields of tissue sections at a magnification of 100x.

Solmaz V., Köse Özlece H., Eroglu H.A., Aktuğ H., Erbaş O, & Taşkıran D. (2017). Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers. Neurology Research International, 2017, 5952149.

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Retinal Morphology Measurement Protocol

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All sections were photographed and measured with the same Olympus C-5050 digital camera mounted on an Olympus BX51 microscope. The mean ONL thickness in the physiological saline groups was accepted as 100%.

Değirmenci C., Afrashi F., Erbaş O., Aktuğ H, & Taşkıran D. (2019). The Preventive Effect of Oxytocin on Retinopathy in Streptozotocin- Induced Diabetic Rats. Turkish Journal of Ophthalmology, 49(2), 68-72.

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Scoring Colon Inflammation in Histology

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Formalin-fixed colon sections (4 μm) were stained with hematoxylin & eosin (H&E). All sections were photographed with an Olympus C-5050 digital camera mounted on an Olympus BX51 microscope.
Scores were assigned using the following criteria of MacPherson and Pfeiffer21 (link) and modifications of this criteria by Fabia et al.22 (link) and MacPherson and Pfeiffer23 (link). Five scores from 0 to 4 were determined. In stage 0, the epithelium is intact and there are no leukocytes or hemorrhage. In stage 1, there are <25% disrupted epithelium, focal leukocyte infiltration, and focal hemorrhage. In state 2, 25% disrupted epithelium, focal leukocyte infiltration, and focal hemorrhage were defined. In stage 3, 50% disrupted epithelium, widespread leukocytes, and hemorrhage were seen. Stage 4 was with >50% disrupted epithelium, extensive leukocyte infiltration, and hemorrhage.

Dibekoğlu C, & Erbaş O. (2022). Histone deacetylase inhibitor givinostat has ameliorative effect in the colitis model. Acta Cirúrgica Brasileira, 37(5), e370503.

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Ovarian Follicle Staging in Formalin-Preserved Tissue

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Formalin-preserved ovarian tissues were embedded in paraffin. The ovaries were sectioned at a thickness of 4 µm using a microtome, and the sections were mounted onto glass slides and stained with hematoxylin and eosin. An Olympus C-5050 digital camera (Olympus, Tokyo, Japan) mounted on an Olympus BX51 microscope was used to photograph all sections. Morphological analysis of the ovaries was performed using a computerized image analysis system (Image-ProExpress 1.4.5, Media Cybernetics Inc., Rockville, MD, USA) on 10 fields of view per section at a magnification of 20×; the observer was blinded to the study groups. Ovarian follicles at different developmental stages were categorized according to standards established by Oktay et al. [25 (link)] (Fig. 1). Primordial follicles are localized immediately below the cortex and contain a single layer of squamous granulosa cells. Primary follicles consist of a single layer of cuboidal granulosa cells. Secondary follicles include multiple layers of granulosa cells, and tertiary follicles are characterized by a stratum granulosum and a fluid filled antral space.

Akdemir A., Zeybek B., Akman L., Ergenoglu A.M., Yeniel A.O., Erbas O., Yavasoglu A., Terek M.C, & Taskiran D. (2014). Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model. Journal of Gynecologic Oncology, 25(4), 328-333.

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Immunohistochemical Evaluation of iNOS and Desmin

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To analyze the immunohistochemical expressions, anti-iNOS and anti-desmin antibodies were used. Paraffin sections were immersed in xylene overnight and incubated in methanol containing 3% H₂O₂ to reduce endogenous peroxidase activity. Sections were heated in sodium citrate solution in a microwave oven at 90 W for 5 min and at 360 W for 15 min. Subsequently, sections were incubated in primary antibodies (anti-desmin, Bioss, bs-1026R, USA; 1/100 and anti-iNOS, Santa Cruz, Sc-651, USA; 1/100) for 24 h at 4ºC. Antibody detection was performed with the Histostain-Plus Bulk kit (Bioss, Inc) against rabbit IgG, and 3,3′ diaminobenzidine (DAB) was used to visualize the final product. Immunoreaction was assessed by light microscopy (Olympus BX-51 light microscope, Olympus C-5050 digital camera) at 40× magnification.

Ersel M., Uyanikgil Y., Akarca F.K., Ozcete E., Altunci Y.A., Karabey F., Cavusoglu T., Meral A., Yigitturk G, & Cetin E.O. (2016). Effects of Silk Sericin on Incision Wound Healing in a Dorsal Skin Flap Wound Healing Rat Model. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 1064-1078.

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Histopathological Evaluation of Organ Tissue

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At the
end of the 28 day exposure period, organs (liver, kidney, heart, lung,
and spleen) were removed under ketamine/xylazine anesthesia after
intracardiac fixation with 4% paraformaldehyde, postfixed for 24 h
and processed for paraffin embedding. Paraffin sections were cut into
5 μm thick slices in microtome (Leica RM 2145) and stained with
routine hematoxylin and eosin (H&E).^{13 (link)−15 (link)} Histopathological
evaluation was assessed by light microscopy (Olympus BX-51 light microscope,
Olympus C-5050 digital camera) at a magnification of ×40.

Köse A., Kaya M., Tomruk C., Uyanıkgil Y., Kishalı N., Kara Y, & Şanlı-Mohamed G. (2023). Evaluation of In Vivo Biological Activity Profiles of Isoindole-1,3-dione Derivatives: Cytotoxicity, Toxicology, and Histopathology Studies. ACS Omega, 8(13), 12512-12521.

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Fluopyram Sensitivity Assay for H. schachtii

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To determine the sensitivity of H.schachtii to fluopyram, the nematodes were incubated for 48 h in a DMSO/water solution containing 0–50 ppm fluopyram. Control treatments received the same amount of DMSO as the 50 ppm variant. About 30 J2 were added to each well of a 96-well plate and incubated with the above described fluopyram concentrations or DMSO control for 48 h with gentle shaking of 30 rpm. For evaluation, 2.5% (v/v) final concentration of 1 M NaOH was added to each well and analyzed with a Leica DM4000 microscope equipped with an Olympus C-5050 digital camera. J2 remaining immobile upon NaOH stimulus were considered to be dead. In a parallel experiment, 10,000 J2 for each concentration were incubated for 48 h on a shaker applying a gentle speed of 30 rpm. After incubation, nematodes were washed in an 11 µm sieve with 500 ml sterile water and transferred to 96-well plates with flat bottom. J2 were then incubated for 6 days in water followed by evaluation with NaOH as described to obtain the percentage of recovered J2.

Schleker A.S., Rist M., Matera C., Damijonaitis A., Collienne U., Matsuoka K., Habash S.S., Twelker K., Gutbrod O., Saalwächter C., Windau M., Matthiesen S., Stefanovska T., Scharwey M., Marx M.T., Geibel S, & Grundler F.M. (2022). Mode of action of fluopyram in plant-parasitic nematodes. Scientific Reports, 12, 11954.

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