To evaluate the influence of SARS-CoV-2/SARS-CoV nsp5 and their mutants on the amount of SEV-induced IFN production in HEK-293T cells, a recombinant VSV-GFP was used, and the IFN bioassay was performed as described previously (55 (link)). The percentage of GFP-positive cells from IFN bioassay was calculated by an LSRFortessa X-20 cell analyzer and analyzed by FlowJo v10.
X 20 cell analyzer
Manufactured by FlowJo
The X-20 cell analyzer is a high-performance instrument designed for accurate and efficient cell analysis. It utilizes advanced flow cytometry technology to rapidly and precisely measure various cellular parameters, including size, granularity, and fluorescence markers. The X-20 is capable of handling a wide range of sample types and provides reliable and consistent data to support your research needs.
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Lab products found in correlation
2 protocols using x 20 cell analyzer
1
Measuring SARS-CoV-2 nsp5 Impact on IFN
2
Apoptosis Analysis of Obinutuzumab and Chemotherapy
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Cells were seeded on 6-well plates at 1 Â 10 6 cells/well and were treated with obinutuzumab and/or doxorubicin and/or Z-VAD-FMK, or with obinutuzumab and/or prednisolone at the indicated concentrations for 3 days. Cells were labeled with the Apo-BrdU TdTmediated dUTP nick end labeling (TUNEL) Assay Kit with Alexa Fluor 488 Anti-BrdU (Thermo Fisher Scientific) in accordance with the manufacturer's protocol. The fluorescence was measured with an LSRFortessa X-20 cell analyzer and analyzed using FlowJo v10 software.
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