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Triamcinolone

Manufactured by Merck Group
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Sourced in Italy

Triamcinolone is a synthetic corticosteroid medication used in laboratory settings. It is a white to off-white crystalline powder. Triamcinolone is used for its anti-inflammatory and immunosuppressant properties in various research applications.

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Lab products found in correlation

Shield1, by Takara Bio (3 mentions) Betamethasone, by Merck Group (3 mentions) Prednisolone, by Merck Group (2 mentions) Beclomethasone, by Merck Group (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Amaxa nucleofector 2, by Lonza (1 mentions) Nucleofector kit 5, by Lonza (1 mentions) Flunisolide, by Merck Group (1 mentions) Prednisone, by Merck Group (1 mentions) Methylprednisolone, by Merck Group (1 mentions) Triamcinolone acetonide, by Merck Group (1 mentions) Fluocinolone acetonide, by Merck Group (1 mentions) Clobetasol propionate, by Merck Group (1 mentions) Chitosan, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Hfob1, by American Type Culture Collection (1 mentions) Mc3t3, by American Type Culture Collection (1 mentions) Morphine, by Merck Group (1 mentions)

9 protocols using triamcinolone

1

Preparation of Chitosan and Triamcinolone Mouthwashes

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For the preparation of Biogel, 95 cc of distilled water was transferred to a 500cc beaker, and placed on a hot plate stirrer. Then the required carbopol was added to water and heated. After that, the methyl paraben and propyl paraben were dissolved in 95% alcohol and the solution was added to the beaker, too. Finally, the required amount of glycerin was added to the sample. Then 0.5 grams of effective ingredients of Chitosan (Germany, Sigma-Aldrich, LMW: wt 50000–190000) or triamcinolone (Iran) emulsion were added to this base gel per 100 ml of gel for preparing the Chitosan and triamcinolone mouthwashes. Finally, 0.5% mouthwash with PH=5.5 was prepared.
Rahmani F., Moghadamnia A.A., Kazemi S., Shirzad A, & Motallebnejad M. (2018). Effect of 0.5% Chitosan mouthwash on recurrent aphthous stomatitis: a randomized double-blind crossover clinical trial. Electronic Physician, 10(6), 6912-6919.
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2

Quantifying NHEJ and HR Repair Activities

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The EJ-DR assay was performed as previously described (Bindra et al., 2013 (link)). EJ-DR reporter cells were plated at 200,000–500,000 cells per well in 10% charcoal-stripped FBS (100–119; Gemini), antibiotic/antimycotic, and DMEM and transfected the next day with siRNA and/or plasmid DNA using Lipofectamine 2000 (11668; Invitrogen) or Lipofectamine RNAiMAX (13778; Invitrogen). After knockdown and expression (24 h), cells were grown in 10% Tet-free FBS (100-800; Gemini), antibiotic/antimycotic, and DMEM. Incorporated I-Sce1 was induced with Shield1 (632189; Clontech) and triamcinolone (T6510; Sigma Aldrich) ligands for 24 h. NHEJ and HR repair activity was assessed 48 h postinduction by quantification of DsRed- and GFP-positive cells on BD FACS Calibur system and analyzed on FlowJo (Tree Star).
Patel D.S., Misenko S.M., Her J, & Bunting S.F. (2017). BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites. The Journal of Cell Biology, 216(11), 3521-3534.
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3

HDR Assays in MCF7 and U2OS Cells

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HDR assays were performed using MCF7 DR-GFP and U2OS EJ-DR cells as previously described (10 (link),19 (link),33 (link)). Briefly, cells were seeded and pretreated with cediranib for 6 to 24 hours. For MCF7 DR-GFP cells, site-specific DSBs were introduced using electroporation with Amaxa Nucleofector II and Nucleofector Kit V (Lonza) to deliver 4 μg of a plasmid encoding the restriction enzyme I-Sce I per 1 × 106 cells. For U2OS EJ-DR cells, DSBs were induced by the addition of triamcinolone (0.5 μM; Sigma) and Shield1 (100 nM; Clontech) to cell culture medium for 24 hours to activate the endogenously expressed I-Sce I enzyme. MCF7 DR-GFP and U2OS EJ-DR cells were analyzed by flow cytometry to quantify GFP+ cells 72 hours after DSB induction.
Kaplan A.R., Gueble S.E., Liu Y., Oeck S., Kim H., Yun Z, & Glazer P.M. (2019). Cediranib suppresses homology-directed DNA repair through down-regulation of BRCA1/2 and RAD51. Science translational medicine, 11(492), eaav4508.
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4

Steroid Solutions for Biomedical Research

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Albumin from human serum (HSA) lyophilized powder, ≥ 97% (agarose gel electrophoresis), was purchased from Sigma Aldrich (Italy). To prepare the stock solution (100 μM), HSA was dissolved in 2 mM phosphate buffer solution (PBS, pH 7.4).
Betamethasone (≥98%), flunisolide (≥97%), prednisolone (≥99%) and triamcinolone were all purchased from Sigma Aldrich (Italy); the stock solutions (3 mM) were prepared by dissolving drugs in a solution of 96% ethanol and PBS (1:1, v/v).
Pontremoli C., Barbero N., Viscardi G, & Visentin S. (2017). Insight into the interaction of inhaled corticosteroids with human serum albumin: A spectroscopic-based study. Journal of Pharmaceutical Analysis, 8(1), 37-44.
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5

Quantitative Analysis of Synthetic Steroids

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Methanol and acetonitrile and formic acid 99.9% (LC-MS grade) and water (HPLC gradient grade) were supplied from VWR (VWR International PBI Srl, Milan, Italy). Methylprednisolone, dexamethasone, prednisolone, fluocinolone acetonide, flumetasone, prednisone, triamcinolone, triamcinolone acetonide, beclomethasone, clobetasol propionate, dexamethasone D4, Methylprednisolone D2, and prednisolone D6 (purity > 98%) were purchased from Sigma-Aldrich (Milan, Italy). A 1000 mg L−1 stock solution was made by dissolving the standard in methanol. From this solution, a 10 mg L−1 work solution was made by dilution in methanol.
Giaccone V., Polizzotto G., Macaluso A., Cammilleri G, & Ferrantelli V. (2017). Determination of Ten Corticosteroids in Illegal Cosmetic Products by a Simple, Rapid, and High-Performance LC-MS/MS Method. International Journal of Analytical Chemistry, 2017, 3531649.
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6

Osteoblast Cell Line Manipulation

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The osteoblast cell lines MC3T3 and hFOB1.19 were purchased from American Type Culture Collection (ATCC, CRL-2593 and CRL11372) and cultured as per the manufacturer’s protocol. Dexamethasone (Sigma-Aldrich, D1756. Saint Louis, MO, USA), Betamethasone (Sigma-Aldrich, B7005. Saint Louis, MO, USA), Triamcinolone (Sigma-Aldrich, 1676000. Saint Louis, MO, USA), Hydrocortisone (Sigma-Aldrich, H0888. Saint Louis, MO, USA), and Beclomethasone (Sigma-Aldrich, B0385. Saint Louis, MO, USA) treatments were performed as described in the experiments. Lentiviral vectors containing CMV-Osterix or Foxc2 siRNA were all purchased from GeneCopeiaTM (Rockville, MD, USA) and applied to the cells as per the manufacturer’s protocols.
Qiao X., Wu X., Zhao Y., Yang Y., Zhang L., Cai X., Ma J.A., Ji J., Lyons K., Boström K.I, & Yao Y. (2023). Cell Transitions Contribute to Glucocorticoid-Induced Bone Loss. Cells, 12(14), 1810.
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7

Glucuronidation Assay for Drug Metabolism

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Aprepitant, aprepitant-13C2,d2, aprepitant-β-glucuronide, 3’-azido-3’-deoxythymidine (AZT), and AZT-3-β-D-glucuronide (AZT-G) were purchased from Toronto Research Chemicals, Inc. (North York, ON, CA). Morphine, Morphine-6-β-D-glucuronide (M-6-G), 10,11-dihydrocarbamazapine, 4-methylumbelliferone (4-MU), triamcinolone, and 4-methylumbelliferyl-β-D-glucuronide hydrate (4-MU-G) were purchased from Sigma-Aldrich (St. Louis, MO). Uridine 5'-diphospho-glucuronic acid (UDPGA) (UGT Rxn mix Solution A), 250 mM Tris-HCl (pH 7.5) buffer mix with 40 mM MgCl2 and 0.125 mg/ml alamethicin (UGT Rxn mix Solution B), UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B4, UGT2B7, UGT2B10, UGT2B15, UGT2B17, UGT control Supersomes, and human intestinal microsomes (HIM) were purchased from BD Biosciences (Woburn, MA). Human liver microsomes (HLM) were processed through Dr. Mary Relling's laboratory at St. Jude Children's Research Hospital (Memphis, TN). Livers were obtained through the Liver Tissue Cell Distribution System (funded by #NO1-DK-9-2310) and the Cooperative Human Tissue Network. Protein concentrations were measured using the Qubit® protein assay kit (Invitrogen Life Technologies, Grand Island, NY).
House L., Ramirez J., Seminerio M., Mirkov S, & Ratain M.J. (2015). IN VITRO GLUCURONIDATION OF APREPITANT: A MODERATE INHIBITOR OF UGT2B7. Xenobiotica; the fate of foreign compounds in biological systems, 45(11), 990-998.
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8

Induction of GFP expression

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U2OS EJDR cells were pretreated with SAHA for 24 hours and 2-HG for 3 days before adding ligands, triamcinolone (0.5 μM, Sigma) and Shield1 (100 nM, Clontech), to media for 24 hours. Cells were analyzed by flow cytometry after 72 hours for GFP signal.
Dow J., Krysztofiak A., Liu Y., Colon-Rios D.A., Rogers F.A, & Glazer P.M. (2021). Vulnerability of IDH1 mutant cancers to histone deacetylase inhibition via orthogonal suppression of DNA repair. Molecular cancer research : MCR.
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9

Regulation of CFB Expression in iPS-RPE Cells

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Expression of CFB (Factor B) by iPS-RPE cells (TLHD1 or 454E2) in the presence of other medication was evaluated by FACS, quantitative RT-PCR or IHC analysis. IFN-c treated iPS-RPE cells exposed to triamcinolone or betamethasone (0.01, 0.1, 1, 10 lg/mL; Sigma-Aldrich Corp.) were used for the in vitro assay.
Sugita S., Makabe K., Fujii S, & Takahashi M. (2018). Detection of Complement Activators in Immune Attack Eyes After iPS-Derived Retinal Pigment Epithelial Cell Transplantation. Investigative ophthalmology & visual science, 59(10).
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