Nanolog fluorometer

Manufactured by Horiba

Sourced in United States

The NanoLog Fluorometer is a compact and sensitive instrument used for measuring fluorescence intensity. It is designed to provide accurate and reliable data for various applications that require fluorescence detection and analysis.

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Lab products found in correlation

Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) Lambda 1050, by PerkinElmer (1 mentions) Nanoled, by Horiba (1 mentions) Uv tbx pmt series detector, by Horiba (1 mentions) Mira3, by TESCAN (1 mentions) Uv 1800 spectrometer, by Shimadzu (1 mentions) C5680, by Hamamatsu Photonics (1 mentions)

7 protocols using nanolog fluorometer

Characterization of Colloidal Nanoparticles

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Cells were suspended in PBS to OD_600nm = 0.5. For ζ potential measurements, 100 mL of each sample was diluted into 900 mL PBS. The measurements were performed on a Malvern Zetasizer Nano ZS (Malvern Instruments, Malvern, U.K.) at RT. Data points given are an average of 3 biological replicates with 3 measurements each.
UV–vis absorption measurements were performed using a Perkin Elmer Lambda 1050 spectrophotometer, with deuterium (180–320 nm) and tungsten (320–3300 nm) lamps, a monochromator and three detectors (photomultiplier 180–860 nm, InGaAs 860–1300 nm, and PbS 1300–3300 nm). Absorption spectra were normalized according to a reference spectrum taken at 100% transmission (without the sample), 0% transmission (with an internal shutter), and in the presence of the reference solvent. For the PL measurements and the excitation profiles an iHR320Horiba NanoLog Fluorometer was employed, equipped with a Xenon lamp, two monochromators, and two detectors (photomultiplier and InGaAs).

de Souza‐Guerreiro T.C., Bondelli G., Grobas I., Donini S., Sesti V., Bertarelli C., Lanzani G., Asally M, & Paternò G.M. (2023). Membrane Targeted Azobenzene Drives Optical Modulation of Bacterial Membrane Potential. Advanced Science, 10(8), 2205007.

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Fluorescence Spectroscopy of DNA Tile Structures

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All steady-state and real-time fluorescence spectra were measured by a Nanolog fluorometer (Horiba Jobin Yvon, L-format, equipped with a CW 450 W xenon light source, thermoelectrically cooled R928 PMT, and fully automated excitation and emission polarizers for anisotropy measurements), with a 1 cm path length quartz cell (Starna Cells, Inc.). All spectra were corrected for the wavelength dependence of the detection system response. A volume of 120 μL of 20 nM tile solution was used for all measurements. Fluorescence emission spectra were collected using 495 nm excitation, 4 nm excitation slits, 505–650 nm emission wavelength range, 5 nm emission slits, and 0.5 s integration. Fluorescence anisotropy was measured with 495 nm excitation, 4 nm excitation slits, 520 nm emission, 5 nm emission slits, and 0.5 s integration. Anisotropy values were calculated from the instrument software FluorEssence for Windows by Horiba Scientific.

Jiang S., Yan H, & Liu Y. (2014). Kinetics of DNA Tile Dimerization. ACS Nano, 8(6), 5826-5832.

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Spectroscopic Characterization of Samples

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UV-vis absorption measurements were performed employing a Perkin ElmerLambda 1050 spectrophotometer, with deuterium (180–320 nm) and tungsten (320–3300 nm) lamps, a monochromator and three detectors (photomultiplier 180–860 nm, InGaAs 860–1300 nm, and PbS 1300–3300 nm). Absorption spectra were normalized according to a reference spectrum taken at 100% transmission (without the sample), 0% transmission (with an internal shutter), and in the presence of the reference solvent. For the PL measurements, an iHR320Horiba NanoLog fluorometer was used, equipped with a xenon lamp, two monochromators, and two detectors (photomultiplier and InGaAs). Samples were excited at 480 nm.

Magni A., Mattiello S., Beverina L., Mattioli G., Moschetta M., Zucchi A., Paternò G.M, & Lanzani G. (2023). A membrane intercalating metal-free conjugated organic photosensitizer for bacterial photodynamic inactivation. Chemical Science, 14(30), 8196-8205.

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Fluorescence Spectroscopy of Hybrid Platforms

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Fluorescence spectroscopy was employed to gather qualitative insights into the internal structure and microenvironment of the prepared systems in HPLC-grade water. Pyrene was used as a hydrophobic probe, capable of being incorporated into the hydrophobic domains of the hybrid platforms. A NanoLog Fluorometer (Horiba Jobin Yvon, Piscataway, NJ, USA) was used to record the pyrene emission spectra, with a laser diode serving as the excitation source (Nano LED, 440 nm, 100 ps pulse width) and a UV TBX-PMT series detector (250–850 nm) from Horiba Jobin Yvon (Piscataway, NJ, USA). A description of the utilized method is presented below.
Colloidal dispersions of P407, P407/Tw80, P407/Tw80/MβCD, and P407/Tw80/HPβCD were prepared at concentrations of 10 mg/mL. Following this, 3 μL of a pyrene stock solution (1 mM) was added to each colloidal dispersion. The dispersions were equilibrated for 24 h and the I₁/I₃ ratio was measured at each hybrid system concentration at temperatures of 25 °C, 37 °C, and 50 °C. Fluorescence spectra were collected in the range of 355–630 nm, with an excitation wavelength of 335 nm. Notably, no excimer formation was observed for the examined solutions.

Saitani E.M., Pippa N., Perinelli D.R., Forys A., Papakyriakopoulou P., Lagopati N., Bonacucina G., Trzebicka B., Gazouli M., Pispas S, & Valsami G. (2024). Fabricating Polymer/Surfactant/Cyclodextrin Hybrid Particles for Possible Nose-to-Brain Delivery of Ropinirole Hydrochloride: In Vitro and Ex Vivo Evaluation. International Journal of Molecular Sciences, 25(2), 1162.

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P3HT Nanoparticle Synthesis and Characterization

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P3HT-NPs were prepared from freshly synthesized P3HT by the reprecipitation method in the absence of surfactants (55 (link)). The polymer was dissolved in tetrahydrofuran, and the solution was added to distilled water (solvent/nonsolvent volume in a 1:20 ratio). By means of differential centrifugation, the NPs were separated into fractions of different sizes and characterized by DLS with a NanoBrook Omni particle size analyzer, with a wavelength of 659 nm in back scattering mode (see also the Supplementary Materials). Ultraviolet (UV)–visible (vis) spectroscopy measurements were carried out using a PerkinElmer Lambda 1050 UV/Vis/NIR spectrophotometer and a Horiba Jobin Yvon NanoLog fluorometer. Scanning electron microscopy images were acquired with a Tescan MIRA3.

Tortiglione C., Antognazza M.R., Tino A., Bossio C., Marchesano V., Bauduin A., Zangoli M., Morata S.V, & Lanzani G. (2017). Semiconducting polymers are light nanotransducers in eyeless animals. Science Advances, 3(1), e1601699.

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Steady-State Absorption and Emission Spectroscopy

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UV-vis-NIR (λ = 250 to 1,100 nm) steady-state absorption spectra were recorded using a Shimadzu UV-1800 spectrometer. Steady-state emission spectra were collected on a Horiba Nanolog fluorometer set to a right-angle detection mode. All measurements were performed in CH₂Cl₂ at 295 K.

Bradley J.M., Coleman A.F., Brown P.J., Huang Y., Young R.M, & Wasielewski M.R. (2023). Harvesting electrons and holes from photodriven symmetry-breaking charge separation within a perylenediimide photosynthetic model dimer. Proceedings of the National Academy of Sciences of the United States of America, 120(48), e2313575120.

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Optical Characterization of Materials

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UV-Vis absorption measurements were performed using a Perkin ElmerLambda 1050 spectrophotometer, with deuterium (180-320 nm) and tungsten (320-3300 nm) lamps, a monochromator and three detectors (photomultiplier 180-860 nm, InGaAs 860-1300 nm, and PbS 1300-3300 nm). Absorption spectra were normalized according to a reference spectrum taken at 100% transmission (without the sample), 0% transmission (with an internal shutter), and in the presence of the reference solvent.
For the PL measurements and the excitation profiles an iHR320Horiba NanoLog Fluorometer was employed, equipped with a Xenon lamp, two monochromators, and two detectors (photomultiplier and InGaAs). Samples were excited at 470 nm.
Time-resolved photoluminescence (TRPL) measurements TRPL measurements were carried out using a femtosecond laser source coupled to a streak camera detection system (Hamamatsu C5680). A Ti:sapphire laser (Coherent Chameleon Ultra II, pulse bandwidths of ∼140 fs, repetition rate of 80 MHz, and maximum pulse energy of 50 nJ) was used to pump a second-harmonic crystal (β-barium borate) to tune the pump wavelength to 470 nm. The measurements here shown were performed recording the first 130 ps of decays, with an IRF of 4.1 ps.
When required, a Peltier cell was used in order to control the samples temperature.

Magni A., Bondelli G., Paternò G.M., Sardar S., Sesti V., D'Andrea C., Bertarelli C, & Lanzani G. (2022). Azobenzene photoisomerization probes cell membrane viscosity. Physical chemistry chemical physics : PCCP, 24(15).

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