Primary antibodies used for indirect immunofluorescence were as follows: for Figures 1 , 4 , S5 , and 6 we used rat anti-ATP7B (S. Lutsenko, Johns Hopkins School of Medicine); For Figures 2 , S3 , S4 , 5 , 7 & 8 we used rabbit anti-ATP7B (#ab124973, Abcam, Cambridge, MA); for Figures 3 and S1 we used rabbit anti-ATP7B (Dr. J. Gitlin, Brown Alpert Medical School, Providence, RI); rabbit anti-aminopeptidase N (APN, #1637, (22 (link)) guinea pig anti-dipeptidyl-peptidase 4 (DPP4, (41 (link))) goat anti-EEA1 (#sc-6415, Santa Cruz Biotechnology, Santa Cruz, CA); mouse anti-TGN38, and -Syntaxin 6 (BD Biosciences, San Jose, CA); mouse anti-LAMP1 (#H4A3-s, Developmental Studies Hybridoma Bank, Iowa City, IA); rabbit anti-Cathepsin D (Dr A. Hasilik, Marburg, Germany); rabbit anti-LC3B (#2775, Cell Signaling Technology, Danvers, MA). Secondary antibodies conjugated to Cy3 or Cy5 were from Jackson ImmunoResearch Laboratories (West Grove, PA), while those conjugated to Alexa 488, -568 or -647 were from Molecular Probes (Eugene, OR). Primary antibodies used for immunoblotting inculded: rabbit anti-ATP7B (#3985) (13 (link)), mouse anti-alpha tubulin, DM1A (Sigma, St. Louis, MO). Secondary antibodies conjugated to horseradish peroxidase (HRP) were from G.E. Healthcare (Buckinghamshire, United Kingdom).
Secondary antibodies conjugated to horseradish peroxidase hrp
Manufactured by GE Healthcare
Sourced in United Kingdom, France
Secondary antibodies conjugated to horseradish peroxidase (HRP) are laboratory reagents used in various immunoassay techniques. These antibodies bind to primary antibodies and are labeled with the enzyme HRP, which can be used to catalyze a colorimetric or chemiluminescent reaction for detection and quantification purposes.
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Lab products found in correlation
2 protocols using secondary antibodies conjugated to horseradish peroxidase hrp
1
Antibody Immunofluorescence Assay Protocol
2
Recombinant MMP-14 and Lumican Interaction Study
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Recombinant human pro-MMP-14 (catalytic domain, amino acids 89–265) was obtained from Merck Millipore (Nottingham, UK). Prior to the enzymatic activity assays, pro-MMP-14 was incubated with APMA (AnaSpec, San Jose, USA) to convert the enzyme in the active form. Recombinant human lumican (57 kDa) and its core protein (37 kDa) were produced as previously described [14 (link), 18 (link)] or purchased from R&D Systems (#2846-LU-050, R&D Systems, MN, USA). Rabbit polyclonal anti-lumican antibody was produced as previously described [14 (link)]. Secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from GE Healthcare (Orsay, France) or from Santa Cruz Biotechnology (Dallas, TX, USA).
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