Analytical grade sodium chloride

HPLC-grade chloroform and methanol were purchased from Crown Scientific (Moorebank, NSW, Australia). Analytical-grade sodium chloride and a Bicinchoninic Acid (BCA) assay kit were purchased from Sigma Aldrich (Sydney, NSW, Australia).

Dimethomorph (98.7%) standard was purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). A water-dispersible granule (WG) containing 50% Dimethomorph was obtained from Zhonggang Wanxiang Agricultural Co., Ltd. (Zhengzhou, China). Formic acid of chromatographic purity was acquired from Fluka (Newport News, VA, USA). Acetonitrile and methanol of HPLC grade were supplied by Thermo Fisher Co., Ltd. (Waltham, MA, USA) Analytical-grade sodium chloride and activated anhydrous sodium sulfate were obtained from Sigma-Aldrich (Steinheim, Germany). The graphitized carbon black adsorbent (GCB, 120–400 mesh) was sourced from ANPEL Laboratory Technologies Inc. (Shanghai, China).
Acetonitrile was used to make standard pesticide stock solutions with a concentration of 1000 mg L⁻¹, which were then kept at −20 °C. Acetonitrile was used to dilute the stock solution to generate the standard working solutions required for fortification and calibration, which were made fresh as needed. The solvent calibration and matrix-matched solutions were made by diluting the stock solutions with Acetonitrile and blank extracts from all matrices, respectively. Before use, all standard solutions were kept at 4 °C.

LMP (from orange peel, esterification degree between 22.0% and 28.0%, product number P9135, galacturonic acid ≥74.0%, methoxyl group ≥6.7% under dry condition, w/w) was supplied by Sigma-Aldrich company (St. Louis, MO, USA). CAS (14.1% protein nitrogen, 90% protein, 5.5% water, 3.8% ash, and 0.02% calcium, w/w) was supplied by Sigma-Aldrich company (St. Louis, MO, USA). Analytical grade sodium chloride, potassium chloride, calcium chloride, and citric acid were supplied by Sigma Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China). All solutions were prepared in distilled water.

The biological reagents used in this study were BSA powder (66430.0 g/mol) (Equitech-Bio, Kerrville, TX, USA) and pharmaceutical grade capsaicin (≥99%) from Capsicum spp. (Shanghai, China). The chemical reagents were glutaraldehyde 25% (Electron Microscopy Science, Hatfield, PA, USA) sodium chloride analytical grade (Merck, Darmstandt, Germany), acetonitrile (≥99.9%) (Sigma-Aldrich, St Louis, MS, USA) and absolute ethanol (≥99.8%) (Merck), calcofluor white water solution 1% (Sigma-Aldrich), rhodamine B powder (Hycel, Jalisco, México), orange acridine (Sigma-Aldrich) and propidium iodine (Sigma-Aldrich).

The biological reagents used in this study were BSA (lyophilized powder, 66 kD) (Equitech-Bio, Kerrville, TX, USA) and pharmaceutical grade capsaicin (≥ 99%) from Capsicum spp. (Handim Chemical Co., Ltd, Shanghai, China). The chemical reagents were glutaraldehyde (25%) (Electron Microscopy Science, Hatfield, PA, USA), sodium chloride analytical grade (Merck, Darmstandt, Germany), acetonitrile (≥ 99.9%) (Sigma-Aldrich, St Louis, MS, USA) and absolute ethanol (≥ 99.8%) (Merck, Darmstandt, Germany). Polyacrylamide gel electrophoresis components, which consisted of acrylamide (40%), tris electrophoresis purity reagent, sodium dodecyl sulfate, ammonium persulfate, and tetramethylethylenediamine (TEMED) (Bio-Rad Laboratories, Richmond, CA, USA), Coomassie brilliant blue G-250 (Sigma-Aldrich, St Louis, MS, USA) and acetic acid glacial HPLC grade (Merck, Darmstandt, Germany) for Coomassie staining solution (CBB). For derivatization of free amino acids methanol HPLC grade (Sigma-Aldrich, St Louis, MS, USA), triethylamine (Sigma-Aldrich, St Louis, MS, USA), and phenyl isothiocyanate (Sigma-Aldrich, St Louis, MS, USA) were used.