Ransod sample diluent

GSH-Px activity in blood hemolysates was determined spectrophotometrically with an automated biochemical analyser RX-Daytona (Randox Laboratories, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967 (link)). Blood hemolysates were diluted 41-fold before being analyzed with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). GSH-Px activity was expressed as units per gram of hemoglobin (U/g Hgb). SOD activity in blood hemolysates was determined spectrophotometrically with an automatic biochemical analyser RX Daytona (Randox Laboratories, Crumlin, UK), using a commercially available Ransod kit (Randox Laboratories, Crumlin, UK), which is based on the original method of McCord and Fridovich (1969 (link)). Before the analyses, samples of hemolysates were diluted 1:200 with the Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories, Crumlin, UK). The activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using an automated hematological analyser ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 (link)).

Activity of GPx in whole blood hemolysates was determined spectrophotometrically with an automated biochemical analyzer RX-Daytona (Randox, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967) . Blood hemolysates were diluted 41-fold before analyses with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). Activity of GPx was expressed as units per gram of hemoglobin (U/g Hgb). Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 ). SOD activity in whole blood hemolysates was determined spectrophotometrically with an automatic biochemical analyzer RX Daytona (Randox Laboratories), using commercially available Ransod kit (Randox Laboratories), which is based on the original method of McCord and Fridovich (1969) . Before analyses samples of hemolysates were diluted 1:200 with Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories). Activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (McCord and Fridovich, 1969 ).