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Cd141 clone ad5 14h12

Manufactured by Miltenyi Biotec
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The CD141 (clone AD5-14H12) is a lab equipment product from Miltenyi Biotec. It is a monoclonal antibody that recognizes the CD141 antigen.

Automatically generated - may contain errors

Lab products found in correlation

Hla dr clone ln3, by Thermo Fisher Scientific (2 mentions) Cd1c clone l161, by BioLegend (2 mentions) Cd14 clone 61d3, by Thermo Fisher Scientific (2 mentions) Facsverse instrument, by BD (1 mentions) Cd11c clone bu15, by BioLegend (1 mentions) Facsaria, by BD (1 mentions)

Spelling variants of this product

CD141 (7 citations) CD141 (AD5-14H12; (4 citations)

2 protocols using cd141 clone ad5 14h12

1

Quantifying Costimulatory Molecule Expression on APCs

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Enriched APCs were stained with antibodies recognizing OX40L (clone ANC10G1; Ancell), ICOSL (clone 136726; R&D Bio-techne), or CD86 (clone FUN-1; R&D Bio-techne), or isotype control, and HLA-DR (clone LN3; eBiosciences), CD14 (clone 61D3; eBiosciences), CD11c (clone Bu15; BioLegend), CD1c (clone L161; BioLegend), CD304 (clone 12C2; BioLegend), and CD141 (clone AD5-14H12; Miltenyi Biotec). Samples were acquired on a FACS Verse instrument (BD Biosciences). Stain index was calculated to quantify the level of expression as follows: stain index = (MFIsample − MFIisotype)/(2 × SDisotype), where MFI indicates mean fluorescence intensity, and SD indicates standard deviation.
Durand M., Walter T., Pirnay T., Naessens T., Gueguen P., Goudot C., Lameiras S., Chang Q., Talaei N., Ornatsky O., Vassilevskaia T., Baulande S., Amigorena S, & Segura E. (2019). Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses. The Journal of Experimental Medicine, 216(7), 1561-1581.
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2

Isolation and Characterization of Immune Cells

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Tonsil or lymph node samples were digested as described previously (Durand and Segura, 2016 (link)). In brief, DC subsets and macrophages were selected by density gradient, enriched by negative selection, and isolated by cell sorting on a FACS Aria instrument (BD Biosciences). Antibodies used were anti-CD11c (clone Bu15; BioLegend), HLA-DR (clone LN3; eBioscience), CD14 (clone 61D3; eBioscience), CD1c (clone L161; BioLegend), CD141 (clone AD5-14H12; Miltenyi Biotec), and CD123 (clone AC145; Miltenyi Biotec).
Durand M., Walter T., Pirnay T., Naessens T., Gueguen P., Goudot C., Lameiras S., Chang Q., Talaei N., Ornatsky O., Vassilevskaia T., Baulande S., Amigorena S, & Segura E. (2019). Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses. The Journal of Experimental Medicine, 216(7), 1561-1581.
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