C57bl 6 b6 h 2b

Male C57BL/6 (B6; H-2b) and BALB/c (B/c; H-2d) mice (8 weeks of age) were procured from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The animal study was authorized by the Institutional Animal Care and Use Committee (IACUC) of Zhejiang University, and to ensure consistent environmental conditions, all mice were reared in a specific-pathogen-free environment with free access to food and water, and subjected to a regular 12 hour light/dark cycle. The research protocols were sanctioned by the IACUC at Zhejiang University (Zhejiang, China).
For the probiotics experiment, the mice were randomly assigned to four groups: the control group (n = 8), the BEZ235 group (n = 8), the probiotics group (n = 8), and the combined group (BEZ235 with 2 × 10⁸ colony-forming units of L. rhamnosus HN001, n = 8). The L. rhamnosus HN001, provided by Nutrition & Biosciences, DuPont, was suspended in 200 µL of phosphate buffer saline and administered orally to the BEZ235-treated mice via gavage twice daily for 2 weeks.

Female C57BL/6 (B6, H-2b) and BALB/c (H-2d) mice (age: 8–12 weeks) were purchased from Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. All animals were bred in a specific pathogen-free facility of the Key Lab of Organ Transplantation Institute with (22°C, 50% air humidity, and air change). Unlimited access to food and water was provided. Mice (weighing: 22–28 g) were used as donor and recipient, respectively. All procedures were approved by the guidelines of the Institutional Animal Care and Use Committee (IACUC).