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Signalfire ecl reagent system

Manufactured by Cell Signaling Technology
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Sourced in United States

The SignalFire ECL reagent system is a chemiluminescent detection solution designed for sensitive and quantitative analysis of proteins. The system includes a proprietary mixture of reagents that generate a luminescent signal upon reaction with horseradish peroxidase (HRP)-labeled antibodies or other HRP-conjugated probes.

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Lab products found in correlation

Mes sds running buffer, by Thermo Fisher Scientific (2 mentions) Nupage 4 10 bis tris polyacrylamide gels, by Thermo Fisher Scientific (2 mentions) Peroxidase conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) 0.2 μm pvdf membrane, by Thermo Fisher Scientific (1 mentions)

2 protocols using signalfire ecl reagent system

1

Western Blot Analysis of Cyanobacterium-Virus Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were resolved on precast NuPAGE® 4%–10% Bis‐Tris polyacrylamide gels in MES SDS running buffer (Life Technologies, Grand Island, NY) under reducing conditions according to the manufacturer's instructions. Proteins were visualized by Coomassie Blue staining or transferred to 0.2 μm PVDF membrane (Invitrogen, Grand Island, NY) for standard Western blotting: primary antibody was polyclonal rabbit anti‐CV‐N antibodies (Molecular Targets Laboratory, CCR, NCI, NIH, Bethesda, MD) used at 1 : 1500 dilution (overnight incubation), and secondary antibody was peroxidase‐conjugated goat anti‐rabbit antibody (Thermo Fisher Scientific Inc.) used at 1:2000 for 1 h. Western blot was detected using the SignalFire ECL reagent system (Cell Signaling Technology Inc., Boston, MA).
O'Keefe B.R., Murad A.M., Vianna G.R., Ramessar K., Saucedo C.J., Wilson J., Buckheit K.W., da Cunha N.B., Araújo A.C., Lacorte C.C., Madeira L., McMahon J.B, & Rech E.L. (2015). Engineering soya bean seeds as a scalable platform to produce cyanovirin‐N, a non‐ARV microbicide against HIV. Plant Biotechnology Journal, 13(7), 884-892.
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2

Protein Expression Analysis by Western Blot

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Samples were resolved on pre-cast NuPAGE® 4–10% Bis-Tris polyacrylamide gels in MES SDS running buffer (Life Technologies, USA) under reducing conditions according to the manufacturer’s instructions. Proteins were visualized by Coomassie Blue staining or transferred to 0.2 µm PVDF membrane (Invitrogen, USA) for standard western blotting: primary antibody was polyclonal rabbit anti-CV-N antibodies (NIH, USA) used at 1:1,500 dilution (overnight incubation), and secondary antibody was peroxidase-conjugated goat anti-rabbit antibody (Thermo Fisher Scientific Inc., USA) used at 1:2000 for 1h. Western blot was detected using the SignalFire ECL reagent system (Cell Signaling Technology Inc., USA).
O'Keefe B.R., Murad A.M., Vianna G.R., Ramessar K., Saucedo C.J., Wilson J., Buckheit K.W., da Cunha N.B., Araújo A.C., Lacorte C.C., Madeira L., McMahon J.B, & Rech E.L. (2015). Engineering soya bean seeds as a scalable platform to produce cyanovirin‐N, a non‐ARV microbicide against HIV. Plant Biotechnology Journal, 13(7), 884-892.
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