Anti cd28 antibody 37.51

Manufactured by Thermo Fisher Scientific

The Anti-CD28 antibody (37.51) is a laboratory reagent used in immunological research. It binds to the CD28 receptor expressed on the surface of T cells, which is a co-stimulatory molecule involved in T cell activation. This antibody can be used to study T cell function and signaling pathways.

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Lab products found in correlation

Anti cd3e 145 2c11, by Thermo Fisher Scientific (1 mentions) Easylyse, by Agilent Technologies (1 mentions) Fvs780, by BD (1 mentions)

Close spelling variants of this product

Anti-CD28 (306 citations) Anti-CD28 antibody (82 citations) Anti-CD28 (37.51; (33 citations) CD28 antibody (10 citations) Anti-CD28 (37.51, (3 citations) CD28 (37.51) (2 citations)

2 protocols using anti cd28 antibody 37.51

T Cell Stimulation Assay

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Cells were placed in culture plates pre-coated with anti-CD3ε a (145-2C11; eBioscience) at concentrations of 0.1 to 10 μg/ml in the presence or absence of anti-CD28 antibody (37.51; eBioscience) at 10 μg/ml. Where indicated, peptide-MHC tetramers (MBL) were added at concentrations of 0.1–5 nM.

Takada K., Van Laethem F., Xing Y., Akane K., Suzuki H., Murata S., Tanaka K., Jameson S.C., Singer A, & Takahama Y. (2015). TCR affinity for thymoproteasome-dependent positively selecting peptides conditions antigen responsiveness in CD8+ T cells. Nature immunology, 16(10), 1069-1076.

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T Cell Proliferation Assay with CFSE

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The splenocytes were isolated, mashing spleen with a plunger end of syringe into a 40-µm cell strainer. The erythrocytes were lysed using Easylyse (Dako). The cells were then labeled with 0.2 μM carboxyfluorescein succinimidyl ester (CFSE; Intercom). A 96-well round-bottom plate was precoated with an anti-CD3e (145-2C11, 5 µg/mL, eBioscience) for 2 h at 37 °C. The T cells (10⁵ cells/well) were added and incubated for 72 h with an anti-CD28 antibody (37.51, 2.5 µg/mL, eBioscience) or complete media for control well in a humidified environment with 5% CO₂ at 37 °C. After 3 d of culture, the cells were harvested and labeled with CD4 (GK1.5), CD8 (53-6.7) antibodies (BD Biosciences), and FVS780 (BD Biosciences) for cell viability. CFSE dilution was assessed by flow cytometry on a Fortessa X-20, and results were analyzed with ModFit LT software.

Reizine F., Grégoire M., Lesouhaitier M., Coirier V., Gauthier J., Delaloy C., Dessauge E., Creusat F., Uhel F., Gacouin A., Dessauge F., Le Naoures C., Moreau C., Bendavid C., Daniel Y., Petitjean K., Bordeau V., Lamaison C., Piau C., Cattoir V., Roussel M., Fromenty B., Michelet C., Le Tulzo Y., Zmijewski J., Thibault R., Cogné M., Tarte K, & Tadié J.M. (2022). Beneficial effects of citrulline enteral administration on sepsis-induced T cell mitochondrial dysfunction. Proceedings of the National Academy of Sciences of the United States of America, 119(8), e2115139119.

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