Cd135 pe

Manufactured by BioLegend

CD135-PE is a fluorescently labeled monoclonal antibody that binds to the CD135 (also known as FLT3) antigen. CD135 is a receptor tyrosine kinase that plays a role in the regulation of hematopoietic stem cell development and differentiation.

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Lab products found in correlation

Cd34 bv421, by BioLegend (4 mentions) Clinimacs, by Miltenyi Biotec (3 mentions) Cd45ra bv711, by BioLegend (3 mentions) Cd38 pecf594, by BD (3 mentions) Lineage cocktail bv510, by BD (3 mentions) Cd36 percpcy5, by BD (3 mentions) Cd110 apc, by BD (3 mentions) Cd41a apch7, by BD (3 mentions) Cd3 apc, by BioLegend (2 mentions) Cd150 pe cy7, by BioLegend (2 mentions) Cd34 fitc, by BioLegend (1 mentions) Cd19 bv605, by BioLegend (1 mentions) Cd48 fitc, by BioLegend (1 mentions) Cd43 pe cy7, by BioLegend (1 mentions) Mouse hematopoietic lineage biotin panel, by Thermo Fisher Scientific (1 mentions) Sca1 apc, by BioLegend (1 mentions) Easysep human progenitor cell enrichment kit, by STEMCELL (1 mentions) Cd34 fitc, by Thermo Fisher Scientific (1 mentions) Cd3 fitc, by BD (1 mentions) Cd19 fitc, by BD (1 mentions)

Spelling variants of this product

CD135 (3 citations)

6 protocols using cd135 pe

Multiparametric Immunophenotyping of Hematopoietic Cells

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Anti- mouse Ter119-BV421, Sca-1-APC, CD34-FITC, CD135-PE, IL-7Ra-BV605, CD48-FITC, CD150-PE Cy7, CD45.2-Alexa Fluor 700/APC Cy7, CD11b-BV605, CD3-APC, NK1.1-APC Cy7, CD19-BV605, IgM-Alexa Fluor 647, CD43-PE Cy7, CD16/32-PerCP Cy5.5, CD16/32; anti-human CD34-APC Cy7 (clone: 581), CD38-PE Cy7 (HB-7), CD45RA-BV 605 (HI100), CD90-APC (5E10), CD49f-PerCP Cy5.5 (GoH3), CD45-PE/Dazzle 594 (HI30), and CD33-APC (P67.6) antibodies; and Legendplex™ Mouse Inflammation Panel (13-plex) and Mouse IL-6 ELISA were purchased from Biolegend (San Diego, CA). IL6 depleting AB (clone MP5–20F3) from BioXCell (West Lebanon, NH).
Anti-mouse c-Kit-PE Cy7, Mouse Hematopoietic Lineage Biotin Panel, and anti-human CD19-Alexa Fluor 700 (HIB19) from eBioscience (San Diego, CA). Anti-B220-PE CF594 from BD biosciences (San Jose, CA). EasySep Human Progenitor Cell Enrichment Kit from Stem Cell Technologies Inc. (Vancouver, BC). Anti-human EPCR-FITC (RCR-49) from Santa Cruz Biotechnology®, inc. (Dallas, TX). Anti-human CD141-PE (AD5–14H12) from Miltenyi Biotec Inc. (Auburn, CA).

Basu S., Liang H.P., Hernandez I., Zogg M., Fields B., May J., Ogoti Y., Wyseure T., Mosnier L.O., Burns R.T., Carlson K, & Weiler H. (2019). Role of Thrombomodulin expression on hematopoietic stem cells. Journal of thrombosis and haemostasis : JTH, 18(1), 123-135.

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Isolation and Characterization of Hematopoietic Progenitor Cells

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Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34+ enriched (CliniMACS; Miltenyi), stained with Lineage cocktail-BV510 (BD), CD34-BV421 (Biolegend), CD38-PECF594(BD), CD45Ra-BV711, CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD) and CD41a-APCH7(BD) antibodies. Human MEP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻-CD41a⁻), ErP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^highCD110⁻), MkP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻CD41a⁺) and CMP (Lin⁻CD34⁺CD45Ra⁻CD135⁺) were sorted on a FACSAria, as previously described (Sanada et al., 2016 (link)).

Xavier-Ferrucio J., Ricon L., Vieira K., Longhini A.L., Lazarini M., Bigarella C.L., Franchi G J.r., Krause D.S, & Saad S.T. (2017). Hematopoietic defects in response to reduced Arhgap21. Stem cell research, 26, 17-27.

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Isolation and Characterization of Hematopoietic Progenitor Cells

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Multiparametric Flow Cytometry Profiling of Hematopoietic Lineages

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Blood lymphoid. CD4-Fitc (BioLegend), CD8-PE-Cy7 (BD), B220–Pacific blue (BD), CD11b-PerCpCy5,5 (BD), CD3-APC (BioLegend); Blood myeloid and erythroid: CD3-Fitc (BD), CD19-Fitc (BD), B220-Fitc (BD), Gr1-PE (BD), F4/80-Pacific blue (BioLegend), CD11b-APC (BD);
BM lymphoid. IgD-Fitc (BD), CD25-PE (BioLegend), CD11b-PerCpCy5,5 (BD), IgM-PECy7 (Southern Biotech), B220-Pacific blue (BD), cKit-APC (BD); BM myeloid: Gr1-PE (BD), CD11b-PerCpCy5,5 (BD), Ter119-PECy7 (BD), F4/80–Pacific blue (BioLegend), CD19-APC (BioLegend); BM megakaryocyte: CD3-Fitc (BD), CD41-PE (BioLegend), CD11b-PerCpCy5,5 (BD), B220–Pacific blue (BD), CD19-APC (BioLegend); HSPC and CLP: Strep-APC/CY7 (Southern Biotech), cKit-APC (BD), SCA1-PacBlue (BioLegend), CD34-FITC (eBioscience), CD135-PE (BioLegend), CD150-PECy7 (BioLegend), CD127-PErCpCy5,5 (BioLegend); Hematopoietic progenitors: Strep-APC/CY7 (Southern Biotech), cKit-APC (BD), SCA1-PacBlue (BioLegend), CD34-FITC (eBioscience), CD16/32-PerCpCy5,5 (eBioscience), CD127-PE (eBioscience).

Alendar A., Lambooij J.P., Bhaskaran R., Lancini C., Song J.Y., van Vugt H., Snoek M, & Berns A. (2020). Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development. PLoS ONE, 15(5), e0233394.

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Isolation of Hematopoietic Progenitor Subsets

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Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34⁺ enriched (CliniMACS; Miltenyi) and stained with Lineage cocktail-BV510 (BD Biosciences [BD]), CD34-BV421 (Biolegend), CD38⁻ PECF594 (BD), CD45Ra-BV711 (Biolegend), CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD), and CD41a-APCH7 (BD) antibodies. Human MEPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁻), MkPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁺), and ErPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38hiCD110⁻) were sorted on a FACSAria, as previously described^{3 (link)}.

Scanlon V.M., Thompson E.N., Lawton B.R., Kochugaeva M., Ta K., Mayday M.Y., Xavier-Ferrucio J., Kang E., Eskow N.M., Lu Y.C., Kwon N., Laumas A., Cenci M., Lawrence K., Barden K., Larsuel S.T., Reed F.E., Peña-Carmona G., Ubbelohde A., Lee J.P., Boobalan S., Oppong Y., Anderson R., Maynard C., Sahirul K., Lajeune C., Ivathraya V., Addy T., Sanchez P., Holbrook C., Van Ho A.T., Duncan J.S., Blau H.M., Levchenko A, & Krause D.S. (2022). Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation. Scientific Reports, 12, 16218.

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Comprehensive Bone Marrow Cell Immunophenotyping

Cited 1 time

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The detailed protocol for whole BM staining has been previously reported (Basso-Ricci et al., 2017 (link)). In brief, Precision Count beads (BioLegend) were added to 100 μl of P1 BM sample to allow absolute quantification of hematopoietic cell subsets, and red blood cell lysis was performed. The lysed sample was labeled with fluorescent antibodies for CD3-BV605, CD56-PE-Cy5, CD14-BV510, CD61/41-PE-Cy7, CD135-PE, CD34-BV421, CD45RA-APC-Cy7 (BioLegend), CD33-BB515, CD66b-BB515, CD38-BUV737, CD45-BUV395, CD90-APC, CD10-BV786, CD11c-BV650, CD19-APCR700, CD7-PE-Cy5.5, and CD71-BV711 (BD Biosciences). Titration assays were performed to assess the best antibody concentration. After surface marking, the cells were incubated with PI (BioLegend) to stain dead cells. All samples were acquired using a BD LSR Fortessa (BD Bioscience) flow cytometer after calibration with SPHERO rainbow calibration particles (Spherotech), and raw data were collected using BD FACSDIVA software. The data were subsequently analyzed with FlowJo software, and the graphical output was automatically generated using GraphPad Prism.

Lam M.T., Coppola S., Krumbach O.H., Prencipe G., Insalaco A., Cifaldi C., Brigida I., Zara E., Scala S., Di Cesare S., Martinelli S., Di Rocco M., Pascarella A., Niceta M., Pantaleoni F., Ciolfi A., Netter P., Carisey A.F., Diehl M., Akbarzadeh M., Conti F., Merli P., Pastore A., Levi Mortera S., Camerini S., Farina L., Buchholzer M., Pannone L., Cao T.N., Coban-Akdemir Z.H., Jhangiani S.N., Muzny D.M., Gibbs R.A., Basso-Ricci L., Chiriaco M., Dvorsky R., Putignani L., Carsetti R., Janning P., Stray-Pedersen A., Erichsen H.C., Horne A., Bryceson Y.T., Torralba-Raga L., Ramme K., Rosti V., Bracaglia C., Messia V., Palma P., Finocchi A., Locatelli F., Chinn I.K., Lupski J.R., Mace E.M., Cancrini C., Aiuti A., Ahmadian M.R., Orange J.S., De Benedetti F, & Tartaglia M. (2019). A novel disorder involving dyshematopoiesis, inflammation, and HLH due to aberrant CDC42 function. The Journal of Experimental Medicine, 216(12), 2778-2799.

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