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Nsmase2a sc 166637

Manufactured by Santa Cruz Biotechnology
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NSmase2a (sc-166637) is a recombinant protein product offered by Santa Cruz Biotechnology. It is an enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide and phosphocholine.

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Lab products found in correlation

Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (2 mentions) Vinculin, by Merck Group (2 mentions) Hrp conjugated actin, by Abcam (2 mentions) Cd63 sc 5275, by Santa Cruz Biotechnology (2 mentions) Cgas sab2100310, by Merck Group (2 mentions) Sting, by R&D Systems (2 mentions) Calnexin, by Abcam (2 mentions)

2 protocols using nsmase2a sc 166637

1

Comprehensive Western Blot Analysis of Exosome Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using RIPA buffer in the presence of protease and phosphatase inhibitors. The centrifuged supernatants were subjected to SDS-PAGE (Criterion™ TGX™) and immunoblotting. Transfer to PVDF membranes was carried out using Trans-Blot Turbo™ Transfer System®. The blots were blocked with 2.5% BSA in PBS containing 0.1% Tween20 before incubating with the following primary antibodies: CD81 (sc-9158, Santa Cruz), CD63 (sc-5275, Santa Cruz), calnexin (ab-22595, Abcam), nSmase2a (sc-166637, Santa Cruz), cGAS (SAB2100310, Sigma-Aldrich), STING (AF6516, R&D), vinculin (V9131, Sigma), HRP-conjugated actin (ab-49906, Abcam). Corresponding peroxidase-conjugated secondary antibodies were used (Jackson ImmunoResearch). Membranes were developed using Super Signal West Dura extended duration substrate (Thermo Scientific, 34076). Original blots are shown in Supplementary Figure 7.
Nandakumar R., Tschismarov R., Meissner F., Prabakaran T., Krissanaprasit A., Farahani E., Zhang B.C., Assil S., Martin A., Bertrams W., Holm C.K., Ablasser A., Klause T., Thomsen M.K., Schmeck B., Howard K.A., Henry T., Gothelf K.V., Decker T, & Paludan S.R. (2019). Intracellular bacteria engage a STING-TBK1-MVB12b pathway to enable paracrine cGAS-STING signaling. Nature microbiology, 4(4), 701-713.
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2

Comprehensive Western Blot Analysis of Exosome Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using RIPA buffer in the presence of protease and phosphatase inhibitors. The centrifuged supernatants were subjected to SDS-PAGE (Criterion™ TGX™) and immunoblotting. Transfer to PVDF membranes was carried out using Trans-Blot Turbo™ Transfer System®. The blots were blocked with 2.5% BSA in PBS containing 0.1% Tween20 before incubating with the following primary antibodies: CD81 (sc-9158, Santa Cruz), CD63 (sc-5275, Santa Cruz), calnexin (ab-22595, Abcam), nSmase2a (sc-166637, Santa Cruz), cGAS (SAB2100310, Sigma-Aldrich), STING (AF6516, R&D), vinculin (V9131, Sigma), HRP-conjugated actin (ab-49906, Abcam). Corresponding peroxidase-conjugated secondary antibodies were used (Jackson ImmunoResearch). Membranes were developed using Super Signal West Dura extended duration substrate (Thermo Scientific, 34076). Original blots are shown in Supplementary Figure 7.
Nandakumar R., Tschismarov R., Meissner F., Prabakaran T., Krissanaprasit A., Farahani E., Zhang B.C., Assil S., Martin A., Bertrams W., Holm C.K., Ablasser A., Klause T., Thomsen M.K., Schmeck B., Howard K.A., Henry T., Gothelf K.V., Decker T, & Paludan S.R. (2019). Intracellular bacteria engage a STING-TBK1-MVB12b pathway to enable paracrine cGAS-STING signaling. Nature microbiology, 4(4), 701-713.
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