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Mes or sds running buffer

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MES or SDS running buffer is a laboratory solution used for electrophoresis, specifically for separating and analyzing proteins or nucleic acids. It provides a buffer environment that allows for the efficient and consistent separation of these biomolecules during electrophoresis experiments.

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Lab products found in correlation

Nupage novex bis tris 4 12 polyacrylamidegels, by Thermo Fisher Scientific (2 mentions) 0.22 micron pvdf, by Merck Group (2 mentions) Bca protein assay, by Thermo Fisher Scientific (2 mentions) Phosstop tablet, by Roche (2 mentions)

Spelling variants of this product

MES buffer (151 citations) MES running buffer (120 citations) MES SDS running buffer (114 citations) MES-SDS buffer (17 citations) Running Buffer (9 citations) SDS running buffer (8 citations) MES or MOPS SDS running buffer (3 citations)

2 protocols using mes or sds running buffer

1

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was extracted from cells and tissue specimens using
modified NP40 lysis buffer (50mM Tris, 150mM NaCl, 1% NP40, 0.1% SDS, 5%
Glycerol supplemented with cOmplete EDTA-free protease inhibitor and Phos-Stop
tablets (Roche)). Tissue specimens were mechanically processed at cryogenic
temperatures using a micro mortar and pestle. Lysates were then sonicated using
a probe sonicator to shear genomic DNA and release lipid-membrane-bound
proteins. The resulting specimen was centrifuged (>10,000 RPM) for 10
minutes at 4°C in a microfuge and supernatant was collected. These total
protein lysates were quantified (Thermo-Pierce BCA protein assay) for equal
loading. Lysates were run on NuPAGE Novex Bis-Tris 4–12% polyacrylamide
gels (Thermo Fisher) with MES or SDS running buffer (Thermo Fisher) and
transferred to 0.22 micron PVDF (Millipore). Blots were incubated overnight in
primary antibody in 5% w/v blotting-grade blocker dissolved in PBS-T and
developed using HRP-labelled secondary antibodies and Amersham ECL or Amersham
ECL prime chemiluminescent detection reagent. A list of antibodies used in this
manuscript is available in Supplementary Data 4 and includes links to manufacturer and external
antibody validation data.
Bader D.A., Hartig S.M., Putluri V., Foley C., Hamilton M.P., Smith E.A., Saha P.K., Panigrahi A., Walker C., Zong L., Martini-Stoica H., Chen R., Rajapakshe K., Coarfa C., Sreekumar A., Mitsiades N., Bankson J.A., Ittmann M.M., O’Malley B.W., Putluri N, & McGuire S.E. (2018). Mitochondrial pyruvate import is a metabolic vulnerability in androgen receptor-driven prostate cancer. Nature metabolism, 1(1), 70-85.
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2

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was extracted from cells and tissue specimens using
modified NP40 lysis buffer (50mM Tris, 150mM NaCl, 1% NP40, 0.1% SDS, 5%
Glycerol supplemented with cOmplete EDTA-free protease inhibitor and Phos-Stop
tablets (Roche)). Tissue specimens were mechanically processed at cryogenic
temperatures using a micro mortar and pestle. Lysates were then sonicated using
a probe sonicator to shear genomic DNA and release lipid-membrane-bound
proteins. The resulting specimen was centrifuged (>10,000 RPM) for 10
minutes at 4°C in a microfuge and supernatant was collected. These total
protein lysates were quantified (Thermo-Pierce BCA protein assay) for equal
loading. Lysates were run on NuPAGE Novex Bis-Tris 4–12% polyacrylamide
gels (Thermo Fisher) with MES or SDS running buffer (Thermo Fisher) and
transferred to 0.22 micron PVDF (Millipore). Blots were incubated overnight in
primary antibody in 5% w/v blotting-grade blocker dissolved in PBS-T and
developed using HRP-labelled secondary antibodies and Amersham ECL or Amersham
ECL prime chemiluminescent detection reagent. A list of antibodies used in this
manuscript is available in Supplementary Data 4 and includes links to manufacturer and external
antibody validation data.
Bader D.A., Hartig S.M., Putluri V., Foley C., Hamilton M.P., Smith E.A., Saha P.K., Panigrahi A., Walker C., Zong L., Martini-Stoica H., Chen R., Rajapakshe K., Coarfa C., Sreekumar A., Mitsiades N., Bankson J.A., Ittmann M.M., O’Malley B.W., Putluri N, & McGuire S.E. (2018). Mitochondrial pyruvate import is a metabolic vulnerability in androgen receptor-driven prostate cancer. Nature metabolism, 1(1), 70-85.
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