Jurkat cells were plated at 1 × 104 cells/well in 96-well plates in 200 μL medium with DAPTA (10 μM). After 2 hr, Jurkat cells were stimulated by con A (4 μg/mL) in 200 μL medium for 12 h. Detection of BrdU incorporation was performed by ELISA (BrdU Cell Proliferation Assay Kit, Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer's instructions.
Elisa brdu cell proliferation assay kit
Manufactured by Cell Signaling Technology
Sourced in United States
The ELISA (BrdU Cell Proliferation Assay Kit) is a laboratory tool designed to measure cell proliferation. It utilizes the incorporation of the synthetic thymidine analog BrdU during DNA synthesis as an indicator of cell division. The kit provides the necessary reagents and materials to perform this measurement.
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4 protocols using elisa brdu cell proliferation assay kit
1
Jurkat Cell Proliferation Assay
2
BrdU Proliferation Assay of SUM-149 Cells
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For 5-bromo-2'-deoxyuridine (BrdU) incorporation detection (ELISA BrdU Cell Proliferation Assay Kit, Cell Signaling Technology, Danvers, MA), 5x104 SUM-149 cells/well were seeded and incubated overnight. After 24h, cells were treated with Erlotinib and/or GLE for 72h, then 10 µM-BrdU was added and after a 22h incubation the cells were fixed, DNA was denatured and fixed. BrdU mouse mAb was added to detect BrdU incorporation via colorimetric detection (450nm). Cell proliferation was calculated as percent of proliferating cells after treatment relative to vehicle wells.
3
CXCL13 Driven RPE Cell Proliferation
4
Comprehensive Cell Proliferation and Migration Assay
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Cell proliferation was measured using BrdU Cell Proliferation ELISA Assay Kit (Cell Signaling). Cell apoptosis was assessed by measuring cytosolic oligonucleosome‐bound DNA using a Cell Death ELISA kit (Roche). The absorbance at 450 nm was measured on a microplate reader (SpectraMax). Migration was assessed using Boyden Chamber assay (Cell Biolabs). In brief, cells were seeded on the topside of the insert in serum‐free media and a medium with 10% serum was placed in the well below. After 6–8 h incubation in a cell culture incubator, migratory cells moved through the pores toward the serum below. These were then were fixed with 10% formalin, stained with Giemsa, and counted under a light microscope.
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