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Anti e2f1 antibody kh95

Manufactured by Santa Cruz Biotechnology
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The Anti-E2F1 antibody (KH95) is a laboratory reagent that can detect the E2F1 transcription factor. E2F1 is a key regulator of cell cycle progression and plays a role in cell proliferation. This antibody can be used in various immunoassays, such as Western blotting, to identify and quantify E2F1 protein levels in biological samples.

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Lab products found in correlation

Alexa fluor 633, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti actin 1 19, by Santa Cruz Biotechnology (1 mentions) Cellytic m lysis reagent, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-E2F1 (34 citations) Anti-E2F1 (KH95; (7 citations) Anti-E2F1 antibody (5 citations) E2F1 antibody (4 citations) E2F1 KH95 (2 citations)

2 protocols using anti e2f1 antibody kh95

1

Immunocytochemistry of EPC1 and E2F1

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Cells grown on coverslips were fixed in 4% paraformaldehyde, permeabilized, and stained with anti-EPC1 (Santa Cruz Biotechnology) or anti-E2F1 antibody (KH95, Santa Cruz Biotechnology). The secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 633 (Molecular Probes) were used for visualization with a LSM 780 confocal laser-scanning microscope (Carl Zeiss). Cell nuclei were stained with DAPI (5 mg/ml).
Wang Y., Alla V., Goody D., Gupta S.K., Spitschak A., Wolkenhauer O., Pützer B.M, & Engelmann D. (2015). Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures. Nucleic Acids Research, 44(1), 117-133.
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2

Western Blot Analysis of Epigenetic and Cell Cycle Regulators

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Total protein was extracted from the cells in CelLytic M™ lysis Reagent (Merck-Sigma-Aldrich, Darmstadt, Germany). Total protein (60 μg) was transferred to nitrocellulose membrane. The membrane was proved with anti-KDM5B antibody as described in IHC, anti-E2F1 antibody (KH95, Santa Cruz Biotechnology, Dallas, TX), anti-E2F2 antibody (L-20, Santa Cruz Biotechnology) and anti-RB antibody (IF8, Santa Cruz Biotechnology). Anti-Actin (I-19, Santa Cruz Biotechnology) was used as a loading control.
Shigekawa Y., Hayami S., Ueno M., Miyamoto A., Suzaki N., Kawai M., Hirono S., Okada K.I., Hamamoto R, & Yamaue H. (2018). Overexpression of KDM5B/JARID1B is associated with poor prognosis in hepatocellular carcinoma. Oncotarget, 9(76), 34320-34335.
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