Cytokine and chemokine levels were measured in the sera collected at PND 25 – 26 and at PND 42. Sera collected at PND 25 – 26 was considered as the baseline timepoint when determining TMEV related responses and for comparing with levels observed at the end of the study (PND 42; 14 dpi). Immune response proteins were measured with Bio-Plex Pro™ Mouse Cytokine 23-plex Assay kit (Bio-Rad, Hercules, CA, USA) to determine concentration levels of 23 cytokines and chemokines (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 [p40], IL-12 [p70], IL-13, IL-17α, IFN-γ, CCL11 [Eotaxin], G-CSF, GM-CSF, CXCL1 [KC keratinocyte-derived chemokine], CCL2 [MCP-1 Monocyte Chemotactic Peptide 1]), CCL3 [MIP-1α Macrophage Inflammatory Protein1α], CCL4 [MIP-1β], CCL5 [RANTES], and TNF-α). Data was processed using the Bio-Plex Manager software program (Bio-Rad version 4.1.1, Hercules, CA, USA).
Bio plex manager software program
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Manager software program is a data analysis tool designed for use with Bio-Rad's Bio-Plex suspension array system. The software provides features for data acquisition, analysis, and reporting of multiplex immunoassay results.
Automatically generated - may contain errors
3 protocols using bio plex manager software program
1
Cytokine and Chemokine Profiling in TMEV Infection
2
Chronic Cytokine and Chemokine Profiling in TMEV
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To evaluate long-term effects of TMEV, we measured cytokine and chemokine levels at the chronic stage of infection, using serum collected 90 dpi from genetically diverse mice. Bio-Plex Pro™ Mouse Cytokine 23-plex Assay kits (Bio-Rad, Hercules, CA) were used to determine the concentrations of 23 cytokines and chemokines in serum (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17 α, IFN-γ, CCL11 [Eotaxin], G-CSF, GM-CSF, CXCL1 [KC {keratinocyte-derived chemokine}], CCL2 [MCP-1 {monocyte chemotactic peptide 1}]), CCL3 [MIP-1α {macrophage inflammatory protein 1α}], CCL4 [MIP-1β], CCL5 [RANTES {regulated upon activation, normal T-cell expressed and secreted}], and TNF-α). Serum was thawed at room temperature before following the Bio-Plex Pro kit protocol. Instrument calibration was performed for each procedure and all samples were assayed in triplicate using Bio-Plex (Luminex) 200 system (Bio-Rad, Hercules, CA). Data was collected with the Bio-Plex Manager software program (Bio-Rad version 4.1.1).
3
Evaluating TMEV-Induced Cytokine Responses
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We used the serum collected before (baseline) and after injection (at 4 d.p.i. and 14 d.p.i.) to evaluate TMEV-induced immune responses. Immune response proteins were measured with Bio-Plex ProTM Mouse Cytokine 23-plex Assay kit (Bio-Rad, Hercules, CA, USA) to determine concentration levels of 23 cytokines and chemokines (IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17α, IFN-γ, CCL11 [Eotaxin], G-CSF, GM-CSF, CXCL1 [KC {keratinocyte-derived chemokine}], CCL2 [MCP-1 {Monocyte Chemotactic Peptide 1}]), CCL3 [MIP-1α {Macrophage Inflammatory Protein 1α}], CCL4 [MIP-1β], CCL5 [RANTES], and TNF-α). Data were processed and analyzed using the Bio-Plex Manager software program (Bio-Rad version 4.1.1, Hercules, CA, USA).
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