Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were fixed, dehydrated and paraffin embedded. Paraffin sections were dewaxed and rehydrated using a routine protocol [22 (link)]. The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4 °C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently confirmed the results.
Anti cd8 mouse antibody
Manufactured by Proteintech
Sourced in United States
The Anti-CD8 mouse antibody is a laboratory reagent used for the detection and analysis of CD8-positive cells. It is a monoclonal antibody that specifically binds to the CD8 surface marker, which is expressed on certain T lymphocytes and other immune cells. This antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and quantify CD8-positive cells in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using anti cd8 mouse antibody
1
Immunohistochemical Profiling of Primary GC Cells
2
Immunohistochemical Analysis of Immune Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissues or collected cells were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol. The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST), anti-Foxp3 rabbit mAb antibody (1:100, CST), anti-cytokeratin mouse mAb antibody (1:100, Abcam) and anti-CD8 mouse antibody (1:100, Proteintech group) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
3
Multiplex Immunohistochemistry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissues or collected cells were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated according to a routine protocol, and then antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST), anti-Foxp3 rabbit mAb antibody (1:100, CST) , anti-Cytokeratin mouse mAb antibody (1:100, Abcam) and anti-CD8 mouse antibody (1:100, Proteintech group) respectively at 4°C overnight, incubation with a secondary antibody, DAB color development, counterstaining, neutral gum sealing and observation were carried out step by step according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned by Panoramic MIDI. Image J was used to count positive staining cells. The results were con rmed independently by two senior pathologist.
4
Immunohistochemical Analysis of GC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol [22] . The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
5
Immunohistochemical Analysis of GC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh tissues for phenotypic assays or collected primary GC cells for functional assays to test separation purity were xed, dehydrated and para n embedded. Para n sections were dewaxed and rehydrated using a routine protocol [22] . The cells underwent antigen repair, neutralization of endogenous catalases, serum blocking, incubation with anti-CD137 rabbit mAb antibody (1:100, CST, USA), anti-Foxp3 rabbit mAb antibody (1:100, CST, USA), anti-cytokeratin mouse mAb antibody (1:100, Abcam, USA) and anti-CD8 mouse antibody (1:100, Proteintech group, China) at 4°C overnight. Cells were incubated with a secondary antibody, and DAB was used for color development. Cells were counterstained, neutral gum sealed and observed according to a standard immunohistochemical operation procedure. PBS was used as a negative control. The stained sections were scanned using Panoramic MIDI. Image J was used to count positively stained cells. Two senior pathologists independently con rmed the results.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!