Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 were purchased from Millipore (Bedford, MA, USA). Recombinant human IL-10 was purchased from R&D Systems (Minneapolis, MN, USA). Phenylarsine oxide (PAO), CD45 PTPase inhibitor and PTPase inhibitor XVIII were purchased from Merck Millipore (Bedford, MA, USA). Fluorescein isothiocyanate-conjugated anti-CD16, phycoerythrin-conjugated anti-CD163, anti-CD11c, -CD80, -CD1a and -CD14, and APC-conjugated anti-CD14 monoclonal antibodies (mAbs) were purchased from BD Pharmingen (San Diego, CA, USA).
Fluorescein isothiocyanate conjugated anti cd16
Manufactured by BD
Sourced in United States
Fluorescein isothiocyanate-conjugated anti-CD16 is a fluorescently labeled antibody that binds to the CD16 receptor on the surface of certain immune cells, such as natural killer cells and a subset of monocytes. It is used in flow cytometry applications to identify and analyze these cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human il 10, by R&D Systems (1 mentions)
Interleukin 4 (il 4), by Merck Group (1 mentions)
Anti cd11c, by BD (1 mentions)
3 amino 9 ethylcarbazole plus substrate chromogen, by Agilent Technologies (1 mentions)
Phycoerythrin conjugated anti cd16, by BD (1 mentions)
Peroxidase labelled secondary antibody, by Agilent Technologies (1 mentions)
Abc elite, by Vector Laboratories (1 mentions)
Isotype matched monoclonal antibody, by BD (1 mentions)
2 protocols using fluorescein isothiocyanate conjugated anti cd16
1
Monocyte Differentiation Induction
2
Immunohistochemical Analysis of CD16 and OX40
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Standard indirect immunoperoxidase procedures were used for immunohistochemistry
(IHC; ABC-Elite, Vector Laboratories, Burlingame, California). Slides were
dewaxed and rehydrated in distilled water. Endogenous peroxidase activity was
blocked using 0.5% H2O2. Sections were incubated with 10% normal goat serum
(DakoCytomation, Carpinteria, California) for 20 minutes and incubated with
primary antibody at room temperature (RT). Primary antibodies used were specific
for CD16 and OX40 (polyclonal anti-CD134/OX40, ab119904, Abcam, Cambridge,
United Kingdom; biotinylated anti-CD16 were purchased from DAKO (Glostrup,
Denmark) and Novocastra (Newcastle, United Kingdom). Fluorescein
isothiocyanate-conjugated anti-CD16, phycoerythrin-conjugated anti-CD16,
Cy5-conjugated anti-CD16, and isotype-matched monoclonal antibody were purchased
from BD Bioscience (San Jose, California), as previously published by our group.22 (link),23 (link) Subsequently, sections were incubated with peroxidase-labelled secondary
antibody (DakoCytomation) for 30 minutes at RT. For antigen visualization,
sections were immersed in 3-amino-9-ethylcarbazole plus substrate-chromogen
(DakoCytomation) for 30 minutes and counterstained with Gill’s hematoxylin.
(IHC; ABC-Elite, Vector Laboratories, Burlingame, California). Slides were
dewaxed and rehydrated in distilled water. Endogenous peroxidase activity was
blocked using 0.5% H2O2. Sections were incubated with 10% normal goat serum
(DakoCytomation, Carpinteria, California) for 20 minutes and incubated with
primary antibody at room temperature (RT). Primary antibodies used were specific
for CD16 and OX40 (polyclonal anti-CD134/OX40, ab119904, Abcam, Cambridge,
United Kingdom; biotinylated anti-CD16 were purchased from DAKO (Glostrup,
Denmark) and Novocastra (Newcastle, United Kingdom). Fluorescein
isothiocyanate-conjugated anti-CD16, phycoerythrin-conjugated anti-CD16,
Cy5-conjugated anti-CD16, and isotype-matched monoclonal antibody were purchased
from BD Bioscience (San Jose, California), as previously published by our group.22 (link),23 (link) Subsequently, sections were incubated with peroxidase-labelled secondary
antibody (DakoCytomation) for 30 minutes at RT. For antigen visualization,
sections were immersed in 3-amino-9-ethylcarbazole plus substrate-chromogen
(DakoCytomation) for 30 minutes and counterstained with Gill’s hematoxylin.
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