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P 2200

Manufactured by Jasco
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Sourced in Japan

The P-2200 is a laboratory centrifuge designed for general-purpose applications. It features a rotor capacity of up to 4 x 50 mL tubes and can achieve a maximum speed of 6,000 rpm. The P-2200 is a compact and durable instrument suitable for a variety of laboratory tasks that require sample separation and concentration.

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Lab products found in correlation

Jmn ecs400, by JEOL (6 mentions) Jmn eca600, by JEOL (5 mentions) C18 cartridges, by Biotage (4 mentions) Rp 18 f254, by Merck Group (3 mentions) Nicolet is5 ft tr spectrometer, by Thermo Fisher Scientific (3 mentions) J 820 spectrometer, by Jasco (3 mentions) Rp 18f254 plates, by Merck Group (3 mentions) Ftir 8700, by Shimadzu (3 mentions) Combiflash rf, by Teledyne (2 mentions) Silica gel 60n, by Kanto Chemical (2 mentions) Jms t100 gcv, by JEOL (1 mentions) Amx 500, by Bruker (1 mentions) Drx 500, by Bruker (1 mentions) S1000 thermal cycler, by Bio-Rad (1 mentions) R axis rapid 2, by Rigaku (1 mentions) Jms t100td, by JEOL (1 mentions) Precoated silica gel 60 f254 plates, by Merck Group (1 mentions) Spark 10m, by Tecan (1 mentions) Jnm ecs400, by JEOL (1 mentions) Irsprit, by Shimadzu (1 mentions)

Close spelling variants of this product

P-2200 polarimeter (26 citations) P-2200 digital polarimeter (3 citations) P-2200 instrument (2 citations)

15 protocols using p 2200

1

Purification and Characterization of Organic Compounds

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All commercial reagents were used as received. Column chromatography was carried out on 60 N silica gel (Kanto Chemicals). Optical rotations were recorded on JASCO P-2200 digital polarimeter. 1H- and 13C-NMR spectra were recorded on Bruker DRX-500 or Bruker AMX-500 spectrometer (500 MHz for 1H-NMR and 125 MHz for 13C-NMR). NMR spectra were recorded in C6D6 (99.5 atom% enriched, Kanto). 1H chemical shifts were reported in δ value based on residual benzene (7.15 ppm) as a reference. 13C chemical shifts were reported in δ value based on benzene (128.0 ppm) as a reference. Data are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad), coupling constant (Hz), and integration. GC-MS analyses were conducted with MS-2010 (Shimadzu). Mass spectra were obtained with a JEOL JMS-T100GCV (EI mode).
Oligonucleotides for polymerase chain reactions (PCRs) were purchased from Hokkaido System Science Co., Ltd. PCRs were performed with a BioRad S1000 thermal cycler.
Sato H., Narita K., Minami A., Yamazaki M., Wang C., Suemune H., Nagano S., Tomita T., Oikawa H, & Uchiyama M. (2018). Theoretical Study of Sesterfisherol Biosynthesis: Computational Prediction of Key Amino Acid Residue in Terpene Synthase. Scientific Reports, 8, 2473.
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2

Spectroscopic Characterization of Compounds

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Optical rotations were measured on a JASCO P-2200 digital polarimeter. CD spectra were recorded on JASCO J-820 spectrometer. Infrared spectra (IR) were obtained with a Thermo Fisher Scientific NICOLET iS5 FT-TR spectrometer from samples in CH2Cl2. NMR spectra were measured on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard, and chemical shifts are stated as δ values. HRMS data were recorded on a JMS-700 MStation quadrupole (FAB) or JMS-T100TD TOF (DART) mass spectrometer. A crystal of the compound was measured on a R-AXIS RAPID II (Rigaku). Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed on Combiflash Rf (Teledyne Isco) with silica gel and C18 cartridges (Biotage, Uppsala Sweden). Preparative HPLC was operated on a GL Science recycling system using an InertSustain C18 column (5 μm, 20 × 250 mm).
Aimaiti S., Suzuki A., Saito Y., Fukuyoshi S., Goto M., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2018). Corymbulosins I−W, Cytotoxic Clerodane Diterpenes from the Bark of Laetia corymbulosa. The Journal of organic chemistry, 83(2), 951-963.
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3

Characterization of Organic Compounds

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Optical rotations were measured on a JASCO P-2200 digital polarimeter. ECD spectra were recorded on a JASCO J-820 spectrometer. Infrared spectra (IR) were recorded on a Thermo Fisher Scientific NICOLET iS5 FT-TR spectrometer with samples in CH2Cl2. NMR spectra were obtained on JEOL JMN-ECA600 and JMN-ECS400 NMR spectrometers with tetramethylsilane as an internal standard, and chemical shifts are indicated as δ values. HRMS data were recorded on a JEOL JMS-700 (FAB) mass spectrometer. MPLC was performed with C18 cartridges (ODS-25, YMC-DispoPack). Preparative HPLC was conducted on a GL Science recycling system using an InertSustain C18 column (5 μm, 20 × 250 mm) and a CHIRAL ART Cellulose-SC column (5 μm, 10 × 250 mm, YMC).
Tsurumi F., Miura Y., Nakano M., Saito Y., Fukuyoshi S., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2019). Spiro[3.5]nonenyl Meroterpenoid Lactones, Cryptolaevilactones G–L, an Ionone Derivative, and Total Synthesis of Cryptolaevilactone M from Cryptocarya laevigata. Journal of natural products, 82(9), 2368-2378.
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4

Analytical Methods for Compound Characterization

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Optical rotations were determined on a JASCO P-2200 digital polarimeter. ECD spectra were measured on a JASCO J-820 spectrometer. Infrared spectra (IR) were recorded with a Thermo Fisher Scientific NICOLET iS5 FT-TR spectrometer with samples in CH2Cl2. NMR spectra were obtained on JEOL JMN-ECA600 and JMN-ECS400 NMR spectrometers with tetramethylsilane as an internal standard, and chemical shifts are stated as δ values. HRMS data were recorded on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were performed on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed on a Combiflash Rf (Teledyne Isco) instrument with silica gel and C18 cartridges (ODS-25, YMC-DispoPack). Preparative HPLC was conducted on a GL Science recycling system using an InertSustain C18 column (5 µM, 20 × 250 mm).
Suzuki A., Saito Y., Fukuyoshi S., Goto M., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2017). Corymbulosins D–H, 2-Hydroxy- and 2-Oxo-clerodane Diterpenes from the Bark of Laetia corymbulosa. Journal of natural products, 80(4), 1065-1072.
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5

Spectroscopic Characterization of Organic Compounds

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Optical rotations were measured on a JASCO P-2200 digital polarimeter. NMR spectra were recorded on JEOL JMN-ECS400 and JMN-ECA600 spectrometers with tetramethylsilane as an internal standard. Chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 1.0 mm thickness; Merck).
Suzuki Y., Saito Y., Goto M., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2017). (−)-Neocaryachine, an Antiproliferative Pavine Alkaloid from Cryptocarya laevigata, Induces DNA Double-Strand Breaks. Journal of natural products, 80(1), 220-224.
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6

Spectroscopic Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were taken on a JEOL JMN-ECA600 spectrometer and JMN-ECS400 spectrometer with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. High-resolution mass spectrometric data were obtained on a JEOL JMS-700 (FAB) or a JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala, Sweden).
Miyake K., Morita C., Suzuki A., Matsushita N., Saito Y., Goto M., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2019). Prenylated Acetophloroglucinol Dimers from Acronychia trifoliolata: Structure Elucidation and Total Synthesis. Journal of natural products, 82(10), 2852-2858.
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7

Characterization of Synthetic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were acquired on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Microwave irradiation experiments were carried out in a dedicated Biotage Initiator 2.5 microwave apparatus. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden). Compounds 9–1316 ,17 (link),20 and 1921 (link) were obtained previously.
Morita C., Kobayashi Y., Saito Y., Miyake K., Tokuda H., Suzuki N., Ichiishi E., Lee K.H, & Nakagawa-Goto K. (2016). Total Synthesis and in Vitro Anti-Tumor-Promoting Activities of Racemic Acetophenone Monomers from Acronychia trifoliolata. Journal of natural products, 79(11), 2890-2897.
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8

Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were recorded on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden).
Miyake K., Suzuki A., Morita C., Goto M., Newman D.J., O’Keefe B.R., Morris-Natschke S.L., Lee K.H, & Nakagawa-Goto K. (2016). Acetophenone Monomers from Acronychia trifoliolata. Journal of natural products, 79(11), 2883-2889.
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9

Spectroscopic Analysis of Chemical Compounds

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Optical rotations were measured on a JASCO P-2200 digital polarimeter in CHCl3. NMR spectra were recorded on JEOL JNM-ECS400 and JNM-ECA600 NMR spectrometers with tetramethylsilane as an internal standard, and chemical shifts are stated as δ values. HRMS data were measured on a JMS-700 (FAB) or JMS-T100TD (DART) mass spectrometer. UV spectra were recorded on a Tecan SPARK 10M in CH3CN–H2O (1:1) except for compound 2 in CH3CN. IR spectra were measured neat on a Shimadzu IRSprit. Preparative HPLC was performed on a GL Science recycling system using InertSustain C18 (5 μm, 20 × 250 mm) and chiral ART Cellulose-SC (5 μm, 250 × 10.0 mm) columns. Analytical and preparative TLC were carried out on precoated silica gel 60 F254 plates (0.25 or 1 mm thickness; Merck) and an NH2 silica gel 60 F254 plate (0.5 mm; Wako). Column chromatography was conducted with silica gel 60 N (Spherical, 63–210 μm, neutral, Kanto Chemical). Analytical and reversed-phase preparative TLC were performed on silica gel 60 RP-18 F254S (0.25 mm, Merck).
Rahim A., Saito Y., Fukuyoshi S., Miyake K., Goto M., Chen C.H., Alam G., Lee K.H, & Nakagawa-Goto K. (2020). Paliasanines A–E, 3,4-Methylenedioxyquinoline Alkaloids Fused with a Phenyl-14-oxabicyclo[3.2.1]octane Unit from Melochia umbellata var. deglabrata. Journal of natural products, 83(10), 2931-2939.
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10

Optical Rotation of CMA Isomers

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R- and S-CMA were dissolved in 1 M HCl to prepare 0.46 and 0.48 w/v% solutions, respectively. The optical rotation of each solution was measured with a P-2200 digital polarimeter (Jasco Corporation, Tokyo, Japan) using a 100-mm quartz cell.
Umino M., Onozato M., Sakamoto T., Koishi M, & Fukushima T. (2023). Analyzing Citramalic Acid Enantiomers in Apples and Commercial Fruit Juice by Liquid Chromatography–Tandem Mass Spectrometry with Pre-Column Derivatization. Molecules, 28(4), 1556.
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