The anti-CTLA-4 Nb (Nb36) wasdeveloped in our previous study [17 (link)]. Anti-CTLA-4 mAb (Rabbit monoclonal, UC10-4F10–11), anti-CD3 mAb (Rabbit monoclonal, SP7), anti-CD56 mAb (Rabbit monoclonal, EPR21827), anti-CD80 mAb (Rabbit monoclonal, EPR1157(2)), anti-CD83 mAb (Rabbit monoclonal, EPR23809–19) and anti-MHC II mAb (Rabbit monoclonal, 6C6) were all purchased from Abcam (Cambridge, UK).
Anti ctla 4 mab
Manufactured by Abcam
Sourced in United Kingdom
Anti-CTLA-4 mAb is a monoclonal antibody that binds to the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) receptor. CTLA-4 is a negative regulator of T-cell activation and is involved in the regulation of the immune response.
Automatically generated - may contain errors
3 protocols using anti ctla 4 mab
1
Antibody Toolkit for Cellular Analysis
2
Fluorescence-based Evaluation of Nb36 Binding to CD8+ T Cells
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Since Nb36 was prepared with a His-tag, an anti His-tag monoclonal antibody (Abcam, UK, Cat No. ab213204) could be utilized to provide a fluorescent signal. After the co-incubation of LPS-Nb36 with activated CD8+ T cells, flow cytometry was implemented to test the His-tag expression in the CD8+ T cells that had been incubated with anti His-tag mAb. The above steps were then repeated, and the samples were stained with 4′, 6-diamidino-2-phenylindole (DAPI, Roche, Almere, The Netherlands). Images were acquired using a fluorescence microscopy.
In another part, these CD8+ T cells incubated with LPS-Nb36 were reincubated with anti-CTLA-4 mAb (Abcam, UK, Cat No. ab210384), and CTLA-4 expression was examined to reflect the specific binding of LPS-Nb36 to CD8+ T cells. Images were acquired using fluorescence microscope.
In another part, these CD8+ T cells incubated with LPS-Nb36 were reincubated with anti-CTLA-4 mAb (Abcam, UK, Cat No. ab210384), and CTLA-4 expression was examined to reflect the specific binding of LPS-Nb36 to CD8+ T cells. Images were acquired using fluorescence microscope.
3
Antibody Panel for Immune Profiling
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The anti-CTLA-4 Nb (Nb36) and anti-CD105 Nb were developed in our laboratory.
Anti-CTLA-4 mAb, anti-CD3 mAb, anti-CD56 mAb, anti-CD80 mAb, anti-CD83 mAb and anti-MHC II mAb were purchased from Abcam (Cambridge, UK).
Anti-CTLA-4 mAb, anti-CD3 mAb, anti-CD56 mAb, anti-CD80 mAb, anti-CD83 mAb and anti-MHC II mAb were purchased from Abcam (Cambridge, UK).
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