Macoy s 5a medium

Manufactured by Thermo Fisher Scientific

Sourced in United States

Macoy's 5A medium is a well-defined culture medium used for the cultivation of bacteria. It provides the necessary nutrients and growth factors to support the growth of various bacterial species. The medium composition is standardized to ensure consistent and reliable results in microbiological applications.

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Macoy' 5A medium (2 citations)

6 protocols using macoy s 5a medium

Quantitative Analysis of Gene Expression

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Macoy's 5A medium and fetal bovine serum (FBS) were from Gibco (Rockford, MD, U.S.A.). PrimeScrip™ RT Master Mix, RNAiso Plus and SYBR Green Premix Ex Taq™ II were from TaKaRa (Dalian, China). MiRNeasy Mini Kit, miScript II RT Kit and miScript SYBR Green PCR Kit were from Qiagen (Duesseldorf, Germany). Matrigel was from BD (New Jersey, U.S.A.). CCK-8 assay kit was from Dojindo Corp (Kyushu, Japan). SuperSignal West Dura Extended Duration Substrate was from Thermo Fisher (IL, U.S.A.). RIPA lysis buffer was from Beyotime (Shanghai, China). Antibodies against ZEB1, MDM2 and GAPDH were bought from Santa Cruz Biotechnology, Inc. (Dallas, U.S.A.).

Zhang F., Xu Y., Ye W., Jiang J, & Wu C. (2020). Circular RNA S-7 promotes ovarian cancer EMT via sponging miR-641 to up-regulate ZEB1 and MDM2. Bioscience Reports, 40(7), BSR20200825.

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Cell Culture Protocols for Cancer Research

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Colorectal cancer SW620, HT29, and LoVo cells; gastric cancer SGC7901 cells; and 293 T cells were purchased from The Cell Bank of Chinese Academy of Sciences at the Shanghai Institute of Cell Biology. Colorectal cancer cells which were derived from primary colorectal cancer tissue were cultured in DMEM/F12 medium (Gibco, Gaithersburg, MD, USA) [28 (link)]; SW620 cells were cultured in L-15 medium (Gibco); HT29 cells were cultured in Macoy’s 5A medium (Gibco); Lovo cells were cultured in F-12 medium (Gibco); SGC7901 and P1 cells were cultured in RPMI-1640 medium (Cellgro, Manassas, USA); and 293 T cells were cultured in DMEM high-glucose medium (Gibco). All media were supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovine serum (Gibco). The cells were cultured at 37 °C in a humidified atmosphere containing 5% CO₂.

Wen Z., Pan T., Yang S., Liu J., Tao H., Zhao Y., Xu D., Shao W., Wu J., Liu X., Wang Y., Mao J, & Zhu Y. (2017). Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting RORβ. Molecular Cancer, 16, 20.

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Cell Proliferation and Apoptosis Assays

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Macoy’s 5A medium and fetal bovine serum (FBS) were from Gibco (Rockford, MD, USA). Dual-Luciferase Reporter Assay System was from Promega (Madison, WI, USA). miRNeasy Mini Kit, miScript II RT Kit and miScript SYBR Green PCR Kit were from Qiagen (Duesseldorf, Germany). PrimeScrip™ RT Master Mix, RNAiso Plus and SYBR Green Premix Ex Taq™ II were from TaKaRa (Dalian, China). Matrigel was from BD (New Jersey, USA). CCK-8 assay kit was from Dojindo Corp (Kyushu, Japan). RIPA lysis buffer was from Beyotime (Shanghai, China). Lipofectamine 3000 and SuperSignal West Dura Extended Duration Substrate were from Invitrogen/Thermo Fisher Scientific (Waltham, USA). APC-Annexin V and propidium iodide were from Sigma (St. Louis, USA).

Qian W., Huang T, & Feng W. (2020). Circular RNA HIPK3 Promotes EMT of Cervical Cancer Through Sponging miR-338-3p to Up-Regulate HIF-1α. Cancer Management and Research, 12, 177-187.

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Apoptosis Pathway Evaluation Protocol

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Propidium iodide, APC-Annexin V and protease inhibitor cocktail were provided by Sigma (St. Louis, MO, USA); CCK-8 test kit was provided by Dojindo Corp (Kyushu, Japan); lipofectamine 3000 and Trizol were from Thermo Fisher Scientific (Waltham, MA, USA); Apoptosis Detection Kit was from Beyotime (Nanjing, China); Dual-Luciferase Reporter Assay System was provided by Promega (Madison, WI, USA); matrigel was from BD (New Jersey, USA); Macoy’s 5A medium was from Gibco (Rockford, MD, USA); SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit was from Fisher BioReagents^® (Fair Lawn, NJ, USA). Antibodies against the Bcl-2 (# 3498), GAPDH (#2118) and Cleaved caspase3 (# 9661S) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA); antibody against the DACT1 was from Novus Biologicals, LLC (Centennial, CO, USA).

Shi X., Huo J., Gao X., Cai H, & Zhu W. (2020). A newly identified lncRNA H1FX-AS1 targets DACT1 to inhibit cervical cancer via sponging miR-324-3p. Cancer Cell International, 20, 358.

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Culturing Cisplatin-Sensitive and Resistant Ovarian Cancer Cells

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Cisplatin-sensitive ovarian cancer cell line (OV2008), and its resistant variant (C13*) were gifts from Professor Benjamin K. Tsang in the Ottawa Health Research Institute, Ottawa, Canada, and the cells were cultured in Macoy’s 5A medium (Gibco, USA) supplemented with 10% (v/v) fetal bovine serum (Gibco) at 37°C in a humidified atmosphere of 5% CO₂.

Lu H., Sun C., Zhou T., Zhou B., Guo E., Shan W., Xia M., Li K., Weng D., Meng L., Xu X., Hu J., Ma D, & Chen G. (2016). HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells. Oncology Research, 23(3), 119-128.

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Cell Culture Protocols for Cervical Cancer

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The cervical cancer cells SiHa, ME‐180, C‐33A, HeLa, HaCat, and 293T were obtained from the American Type Cancer Culture (ATCC). SiHa, C‐33A, HeLa, and 293T were inoculated with DMEM (HyClone), HaCat was inoculated with MEM (Gibco, Thermo Fisher Scientific), and ME‐180 was inoculated with Macoy's 5A medium (Gibco, Thermo Fisher Scientific) containing 10% fetal bovine serum (Gibco, Thermo Fisher Scientific), 100 units/mL penicillin, and 100 mg/mL streptomycin (HyClone) and cultured in an incubator at 37°C with 5% CO₂ and saturated humidity. After cells had grown along the dish wall, the medium was changed every 1‐2 days and 0.25% trypsin (Sigma‐Company) was used for digestion and subculture. All cells were tested for mycoplasma by a PCR‐based method as well as DAPI staining to ensure the absence of contamination.

Liu H., Zhu C., Xu Z., Wang J., Qian L., Zhou Q., Shen Z., Zhao W., Xiao W., Chen L, & Zhou Y. (2020). lncRNA PART1 and MIR17HG as ΔNp63α direct targets regulate tumor progression of cervical squamous cell carcinoma. Cancer Science, 111(11), 4129-4141.

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