Texas red conjugate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Texas Red conjugate is a fluorescent label used for various biological applications. It has an excitation maximum at 596 nm and an emission maximum at 615 nm, making it suitable for detection in the orange-red region of the visible spectrum. The conjugate can be used to label proteins, nucleic acids, and other biomolecules for various imaging and detection techniques.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Biotinylated wfa, by Vector Laboratories (1 mentions) Dab peroxidase substrate, by Merck Group (1 mentions) Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions) Anti neun antibody, by Abcam (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Mitotracker red, by Thermo Fisher Scientific (1 mentions) Lysotrackerr red dnd 99, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Zen software, by Zeiss (1 mentions) Nocodazole, by Merck Group (1 mentions) Ab6061, by Abcam (1 mentions) Yl1 2, by Abcam (1 mentions) Lifeact rfp, by Thermo Fisher Scientific (1 mentions) Cytochalasin d, by Merck Group (1 mentions) A1plus rsi scanning confocal microscope, by Nikon (1 mentions) Alexa fluor 647 conjugate, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate dextran, by Merck Group (1 mentions)

Close spelling variants of this product

Phalloidin-conjugate to Texas red ConA-Texas Red conjugate Texas Red™-X Conjugate WGA Texas Red-X Conjugate Texas Red

7 protocols using texas red conjugate

Multifunctional Biomolecular Labeling Protocol

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Agarose, low electroendosmosis was purchased from Alfa Aesar (Ward Hill, MA, USA). Av, FITC conjugate, Av, Texas Red conjugate, NeutrAvidin (Nu), FITC conjugate, Nu, Texas Red conjugate, Calcein AM, and Ethidium Homodimer-1 were purchased from Invitrogen (Eugene, OR, USA). Protease Inhibitor Mini Tablets, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride), Sulfo-NHS (N-hydroxysulfosuccinimide), Quant-iT PicoGreen dsDNA Assay Kit, and Dimethyl Sulfoxide (DMSO) were purchased from Thermo Scientific Pierce (Rockford, IL, USA). Trypsin–EDTA phenol red, high glucose DMEM, and Pen-Strep Antibiotic–Antimycotic were purchased from Gibco (Carlsbad, CA, USA). Proteinase-K was purchased from Roche Diagnostics (Risch-Rotkreuz, Switzerland). Nonfat-dried bovine milk, Fluorescein isothiocyanate-Dextran (FITC-Dextran), Fluorescein isothiocyanate isomer 1, Bovine Serum Albumin, 4-(Dimethylamino)pyridine (DMAP), Pyridine, Succinic Anhydride (SA), 2-(N-Morpholino)ethanesulfonic acid (MES), Dimethylmethylene Blue (DMMB), and other salts and reagents were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Atlanta Biologics. Nitric oxide (NO) Griess Reagent System was purchased from Promega (Madison, WI, USA).

Wagner E.K., Vedadghavami A., Jacobsen T.D., Goel S.A., Chahine N.O, & Bajpayee A.G. (2020). Avidin grafted dextran nanostructure enables a month-long intra-discal retention. Scientific Reports, 10, 12017.

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Immunohistochemical Analysis of Brain Sections

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In Experiment 4 (Figure 4), subjects were anesthetized with ketamine-domitor 4hrs or 24 hours after fear conditioning and transcardially perfused with 0.1M PBS followed by 4% PFA. Brains were dissected out and stored in 4% PFA (24 hours) followed by 30% sucrose in 0.1M PBS (72 hours). Brains were sectioned on a Leica microtome at 50um thickness and sections were stored in 0.1MPBS. Every eighth section was processed for WFA immunoreactivity. After extensive washing, sections were incubated overnight at 4 °C in biotiny lated WFA (1:500; Vector labs) or anti-NeuN antibody (1:500, abcam) or anti-Parvalbumin antibody (1:500, Sigma) in PBS and 0.1% Triton X-100. After three washes in PBS, tissue sections were either visualized using VectaStain ABC kit (Vector Laboratories) and developed in DAB peroxidase substrate (Sigma) or exposed to fluorescent secondary antibodies; streptavidin, Texas Red conjugate or Alexa Fluor 488 conjugate (Life technologies). Sections were mounted on Fisherbrand electrostatic slides and coverslipped.

Banerjee S.B., Gutzeit V.A., Baman J., Aoued H.S., Doshi N.K., Liu R.C, & Ressler K.J. (2017). Perineuronal nets in the adult sensory cortex are necessary for fear learning. Neuron, 95(1), 169-179.e3.

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Tracking Cellular Localization of Coumarin 6-Labeled SPIONs

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In this study, to track SPION instead of Dtxl, Coumarin 6, which serves as green fluorescent marker, was encapsulated. To determine the cellular localization of Coumarin 6 labeled SPION (C6-SPION), C4-2 and PC-3 cells were seeded at a density of 2×10⁴ on 8-well chamber glass slides (Nalgene Nunc Intl., Rochester, NY). Cells were then treated with 2 μg of Coumarin 6 equivalent C6-SPION for 3 hrs. After 2 hrs of incubation, cells were fixed using 2% paraformaldehyde for 10 min, permeabilized with 0.1% TritonX-100 in 1X PBS for 10 min, and washed twice with an additional 1X PBS, followed by a blocking step with 2% goat serum in PBS for 1 hr. Then cells were incubated with 30 nM Mito Tracker Red or 50 nM Transferrin from Human Serum, Texas Red^® Conjugate or 75nM LysoTrackerR Red DND-99 (Life Technologies) to stain as a marker for mitochondria, endosome, and lysosome, respectively. Finally, nuclei were counter stained with DAPI (4′,6-diamidino-2-phenylindole, Life Technologies) and mounted in Vectashield Mounting Medium (Vector Labs, Burlingame, CA). Finally, cells were visualized under a laser confocal microscope (Carl Zeiss LSM 710, Oberkochen, Germany) under 400X magnification using oil immersion objective.

Nagesh P.K., Johnson N.R., Boya V.K., Chowdhury P., Othman S.F., Khalilzad-Sharghi V., Hafeez B.B., Ganju A., Khan S., Behrman S.W., Zafar N., Chauhan S.C., Jaggi M, & Yallapu M.M. (2016). PSMA targeted docetaxel-loaded superparamagnetic iron oxide nanoparticles for prostate cancer. Colloids and surfaces. B, Biointerfaces, 144, 8-20.

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Visualizing Ubiquitin Localization in Live and Fixed Cells

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HeLa and RCC4 cells were cultured in DMEM supplemented with 10% fetal bovine serum in a glass bottom dish. Cells were treated with 10 μM ubiB for 6 hrs and washed three times with PBS buffer. The plasma membrane was visualized by treating cells with a 1:500 dilution of wheat germ agglutinin, Texas Red^® conjugate (Life Technologies) for 15 min. A Zeiss LSM700 inverted confocal microscope was used to image live cells in Z-stack configuration at 1 μM increments with a 63× oil immersion lens. Images were acquired at 512×512 pixels with the 405 nm laser for excitation of ubiB and the 555 nm laser to excite the red plasma membrane probe. All images were processed using Zen software (Zeiss) and analyzed with the Fiji distribution of ImageJ.
HeLa cells grown in media containing 10 μM ubiB for 6 hrs on a glass coverslip were used for immunofluorescence. Following treatment, cells were fixed in ice cold 100% (v/v) methanol for 5 min at room temperature, permeabilized in PBS, 15% (v/v) Triton X-100, and blocked overnight at 4°C in PBST buffer containing 1% (w/v) BSA, 22.52 mg/mL glycine, and 0.1% Tween 20. The same four Ub antibodies (see above) were used with an anti-rabbit-Cy5 as the secondary antibody. Throughout this procedure ubiB remained within the cells. Cover slips were mounted to microscope slide and sealed. Images were acquired and analyzed as above in the live cell assay.

Nakasone M.A., Lewis T.A., Walker O., Thakur A., Mansour W., Castañeda C.A., Goeckeler-Fried J.L., Parlati F., Chou T.F., Hayat O., Zhang D., Camara C.M., Bonn S.M., Nowicka U.K., Krueger S., Glickman M.H., Brodsky J.L., Deshaies R.J, & Fushman D. (2017). Structural basis for the inhibitory effects of ubistatins in the ubiquitin-proteasome pathway. Structure (London, England : 1993), 25(12), 1839-1855.e11.

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Integrin Activation Modulation and Visualization

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Antibodies to human β1 integrin used as ligands were 12G10 (ref. 33 (link); Serotec, MCA 2028, 10 μg ml⁻¹), which stabilizes the active integrin conformation, and 4B4 (Beckman Coulter, 6603113, 10 μg ml⁻¹), which stabilizes the inactive integrin conformation. Both 12G10 and 4B4 are the same isotype (IgG1). Other antibodies used were directed against α-tubulin (YL1/2; Abcam, ab6061, 1:1,000 for IF), ACF7 (CU119; provided by R. K. Liem, 1:2,000 for WB), β1 integrin (JB1A; provided by J. A. Wilkins, 1:1,000 for WB), CKAP5 (E-17; Santa Cruz Biotechnology, sc240235, 1:200 for WB), EB1 (Santa Cruz Biotechnology; H-70, sc-15347, 5 μg ml⁻¹ for IF or 1A11, sc-47704, 1:1,000 for WB), talin (C-20; Santa Cruz Biotechnology, sc-7534, 1:2,000 for WB), vinculin (hVIN-1–FITC conjugate; Sigma-Aldrich, F-7053, 1:500 for IF) and FN (39B6; 5 μg ml⁻¹)54 (link). Actin was visualized with phalloidin–Texas Red conjugate (Life Technologies, T7471, 1:500 for IF) or Lifeact–RFP (provided by R. Wedlich-Söldner). Tubulin was visualized with GFP–ensconsin (provided by S. Woolner). Species-specific fluorescent dye-conjugated secondary antibodies were obtained for IF from Jackson Immunoresearch (1:400), and FN, PDL, cytochalasin D and nocodazole were from Sigma-Aldrich.

Byron A., Askari J.A., Humphries J.D., Jacquemet G., Koper E.J., Warwood S., Choi C.K., Stroud M.J., Chen C.S., Knight D, & Humphries M.J. (2015). A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting. Nature Communications, 6, 6135.

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Transferrin Trafficking in U2OS Cells

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U2OS cells were seeded at 20,000 cells per well in 24 well round-bottom plates on glass coverslips and transfected with siRNA pools against non-targeting control, PIK3C2A, ATG9A/B, or PIK3C3 as described above. After 48 hours, cells were treated with rapamycin (50 nM in DMSO) for 6 hours. Cells were then treated with 25 μg/mL transferrin from human serum, Texas Red conjugate (Thermo Fisher, T2875) at 4°C for 5 minutes, washed with fresh media, and returned to 37°C and 5% CO₂ for 0–45 minutes. Cells were fixed and stained as described above. Coverslips were imaged on an A1plus-RSi scanning confocal microscope (Nikon) using 403, 488, and 561 excitation lasers and a 60× oil immersion lens. Images were analyzed using NIS-Elements Software. Individual cells were manually outlined and regions of interest were defined generating a binary ROI of transferrin localization. Overlap between endogenous antibodies and Texas Red-transferrin was calculated by quantifying the overlap of the green channel with the ROI. Results were analyzed using GraphPad Prism. Significance was determined using an unpaired t test using control and experimental siRNA knockdown.

Merrill N.M., Schipper J.L., Karnes J.B., Kauffman A.L., Martin K.R, & MacKeigan J.P. (2017). PI3K-C2α knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE, 12(9), e0184909.

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Multicolor Protein and Particle Labeling

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Albumin from bovine serum (BSA), FITC conjugate (Catalog # A23015, ThermoFisher); Transferrin from human serum, Texas Red® Conjugate (Catalog # T2875, ThermoFisher); Fibrinogen from Human plasma, Alexa Fluor® 647 Conjugate (Catalog # F35200, ThermoFisher); Insulin-FITC conjugate labeled human recombinant (Catalog # I3661, Sigma Aldrich); Hen egg lysozyme, HEL-FITC (LS1-FC-1, Nanocs); Staphyloccocus aureus (SA particles) Alexa-Fluor 647; 0.2 and 2 um beads labeled Alexa-Fluor 647 (Molecular Probes).

Clement C.C., Wang W., Dzieciatkowska M., Cortese M., Hansen K.C., Becerra A., Thangaswamy S., Nizamutdinova I., Moon J.Y., Stern L.J., Gashev A.A., Zawieja D, & Santambrogio L. (2018). Quantitative Profiling of the Lymph Node Clearance Capacity. Scientific Reports, 8, 11253.

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