Cd45 bv605

Manufactured by BioLegend

Sourced in United States

CD45-BV605 is a fluorochrome-conjugated antibody that binds to the CD45 surface antigen, which is expressed on all nucleated hematopoietic cells. It can be used in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd4 fitc, by BioLegend (4 mentions) Cd11b apc, by BioLegend (4 mentions) Epcam af488, by Cell Signaling Technology (3 mentions) Her2 af488, by BioLegend (3 mentions) Egfr fitc, by GeneTex (3 mentions) Diva software, by BD (2 mentions) Cd19 pe cf594, by BD (2 mentions) Cd3 pe cf594, by BD (2 mentions) Cd49b pe cf594, by BD (2 mentions) Cd11c bv650, by BioLegend (2 mentions) F4 80 alexafluor 700, by BioLegend (2 mentions) Cd3 bv650, by BioLegend (2 mentions) Cd8 apc fire, by BioLegend (2 mentions) Propidium iodide, by BioLegend (2 mentions) Cd31 pe, by Thermo Fisher Scientific (2 mentions) Prom1 biotin, by Thermo Fisher Scientific (2 mentions) Egf alexafluor 647, by Thermo Fisher Scientific (2 mentions) Cd24 pacblue, by Thermo Fisher Scientific (2 mentions) Strep pecy7, by Thermo Fisher Scientific (2 mentions) O4 pe, by Miltenyi Biotec (2 mentions)

Close spelling variants of this product

Anti-CD45 BV605 (8 citations) CD45-BV605 (30-F11), (5 citations) CD45.1–BV605 (3 citations) CD45 – BV605 (HI30; (2 citations) BV605-conjugated CD45 (2 citations) Anti-mouse CD45-BV605 (2 citations) Bv605-CD45 (clone 30-F11, (2 citations)

25 protocols using cd45 bv605

Comprehensive Tumor Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor cells isolated from mice were thawed and stained with LIVE/DEAD Fixable Violet Dead Staining Kit (ThermoFisher). Subsequently, the cells were divided and stained with cocktails of fluorochrome-conjugated monoclonal antibodies: CD3 PE-CF594, CD19 PE-CF594, CD49b PE-CF594 (all from BD Biosciences), CD45 BV605, CD11b PerCP-Cy5.5, CD11c BV650, F4/80 AlexaFluor 700, Ly6C PE, Ly6G APC-Cy7, MHC II FITC, CD80 PE-Cy7 (all from Biolegend) for myeloid cell identification and CD45 BV605, CD3 BV650, CD4 FITC, CD8 APC-Fire, CD25 PE, CD44 PE-Cy7, CD62L PerCP-Cy5.5 (all from BioLegend) for lymphocytes identification. Then, the cells were fixed using FoxP3 Fixation Permeabilization Staining Kit (eBioscience). Tumor cells stained with myeloid or lymphocyte cocktail were additionally incubated with anti-CD206 APC (BioLegend) or FoxP3 APC (eBioscience) antibodies, respectively. The analysis was performed using FACSFortessa flow cytometer with Diva software (Becton Dickinson).

Rossowska J., Anger N., Szczygieł A., Mierzejewska J, & Pajtasz-Piasecka E. (2018). Reprogramming the murine colon cancer microenvironment using lentivectors encoding shRNA against IL-10 as a component of a potent DC-based chemoimmunotherapy. Journal of Experimental & Clinical Cancer Research : CR, 37, 126.

+ Open protocol

+ Expand

Tumor-Targeted IL-10 Silencing in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight to ten-week old female C57BL/6 mice were subcutaneously inoculated in the right flank with MC38/0 cells (1.1 × 10⁶/0.2 ml/mouse). On the 14th, 15th and 17th day of the experiment, mice were injected i.t. with LVs encoding shRNA against IL-10 (shIL10–3, 2x10⁶TU/50 μl/mouse) or reference LVs encoding scrambled shRNA against human GAPDH (shN). Two days after the third injection, the mice were sacrificed and their tumor nodules were dissected and homogenized. Efficacy of transduction in tumors was measured by flow cytometry as the fluorescence intensity of EGFP among cells isolated from tumors. Concentration of IL-10 was estimated by ELISA in supernatants collected from 24 h culture of 5 mg tumor tissue/ml. Myeloid and lymphocyte populations in tumors were analyzed using LSR Fortessa with Diva software (Becton Dickinson) after staining with fluorochrome-conjugated antibodies: CD45 V500, CD3 PE-CF594, CD19 PE-CF594, CD49b PE-CF594 (all from BD Biosciences), CD11b PerCP-Cy5.5, CD11c BV650, F4/80 AlexaFluor 700, Ly6C BV510, Ly6G BV605, MHC II APC-Cy7, for myeloid cell identification (all from Biolegend) and CD45 BV605, CD3 BV650, CD4 FITC, CD8 APC-Fire, (all from BioLegend) for lymphocyte identification.

+ Open protocol

+ Expand

Phenotypic analysis of aNSCs/NPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sub-ventricular zone was isolated and dissociated as described above for “Single cell RNA-seq using the 10x Genomics Chromium single cell technology”. In some cases, mice were given a 1mg/mouse dose of 5-ethynyl-2’-deoxyuridine (EdU) via intraperitoneal injection 4 hours prior to euthanasia. Antibody staining was carried out in FACS buffer at the following dilutions: PROM1-Biotin (eBioscience Cat.#13–1331-80 [1:300]), EGF-AlexaFluor 647 (Life Technologies Cat. #E35351 [1:300]), CD24-PacBlue (eBioscience Cat.#48–0242-80 [1:400]), CD31-PE (eBioscience Cat.#12–0311-81 [1:50]), CD45-BV605 (Biolegend Cat.#103139 [1:100]), Strep-PECy7 (eBioscience Cat.#25–4517-82 [1:500]), O4-PE (Miltenyi Cat.#130–099-211 [1:50]), CD317-FITC (Biolegend Cat.#127002 Clone:927 [1:50]) for one hour at 4°C. Samples were washed with 5mL of FACS buffer. Secondary staining was performed using Strep-PECy7 (eBioscience Cat.#25–4517-82 [1:500]) in FACS buffer at 4°C. Samples were washed with 5mL of FACS buffer, and resuspended in media containing 1μg/mL propidium iodide (Biolegend). Fluorescent-minus-one controls were used to set positive gates in each experiment. Cell populations were defined as follows:
BST2-positive and BST2-negative aNSCs/NPCs were subjected to subsequent analyses including bulk RNA-sequencing, EdU cycle analysis, and STAT1 staining.

Dulken B.W., Buckley M.T., Negredo P.N., Saligrama N., Cayrol R., Leeman D.S., George B.M., Boutet S.C., Hebestreit K., Pluvinage J.V., Wyss-Coray T., Weissman I.L., Vogel H., Davis M.M, & Brunet A. (2019). Single cell analysis reveals T cell infiltration in old neurogenic niches. Nature, 571(7764), 205-210.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adherent cells or EBs were dissociated to form a single-cell suspension by TrypLE (Life Technologies) treatment, and washed with FACS buffer (1% FBS and 1 mM EDTA in PBS). The dissociated cells were resuspended in FACS buffer, and labeled with fluorochrome-conjugated anti-human CD34-APC (clone# AC136, Miltenyi Biotec), CD31-PE (clone# AC114.5, Miltenyi Biotec), CD144-Alexa Fluor 700 (clone# 16B1, eBioscience), CD45-BV605 (clone# HI30, BioLegend), CD41-APC/Cy7 (clone# HIP8, BioLegened), CD235a-PE (clone# HIR2, eBioscience), CD43-BV421 (clone# 1G10, BD Biosciences), CD73-PE/Cy7 (clone# AD2, BioLegend), CD117-PE/Cy7 (clone# 104D2, BioLegend), CD127-FITC (clone# eBioRDR5, eBioscience), CD14-FITC (clone# 61D3, eBioscience), or KDR-PerCP (clone# HKDR-1, BioLegend). Flow cytometry was performed on LSR II analyzer (BD Biosciences). Data analysis was performed using FlowJo software or FCS Express software.

BAI H., LIU Y., XIE Y., HOYLE D.L., BRODSKY R.A., CHENG L., CHENG T, & WANG Z.Z. (2015). Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells. Journal of cellular physiology, 231(5), 1065-1076.

+ Open protocol

+ Expand

Mitochondrial Potential in Circulating EPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Basically, ΔΨ_m of EPCs in the circulation of mice was estimated using flow cytometry and fluorescent probe 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolcarbocyanine iodide (JC‐1, no. 551302, BD, Faranklin Lakes, NZ). Samples were collected as previously described in FACS fraction and then incubated with antibodies for CD45‐BV605 (no. 103139; Biolegend), CD34‐BV421 (no. 119321; Biolegend), and VEGFR2‐APC (no. 136406; Biolegend) for 1 hour. The red blood cells were lysed. Samples were stained with 2.5 μM JC‐1 37°C for 15 minutes, washed, resuspended with the assay buffer, and ΔΨ_m of CD34+/CD45−/VEGFR2+ populations were analyzed by flow cytometry immediately.

Shao Y., Chen J., Freeman W., Dong L., Zhang Z., Xu M., Qiu F., Du Y., Liu J., Li X, & Ma J. (2019). Canonical Wnt Signaling Promotes Neovascularization Through Determination of Endothelial Progenitor Cell Fate via Metabolic Profile Regulation. Stem Cells (Dayton, Ohio), 37(10), 1331-1343.

+ Open protocol

+ Expand

Multicolor Flow Cytometry for Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometer was used to detected T cell cytotoxicity, T cell and neutrophil fraction in tissue or as cell counting. For culturing cells, cells were digested with Trypsin followed by antibody marking. For tissue sample, cells were pre-digested with collagenase till in single cell state, then followed by antibody marking. Antibody used in this manuscript include CD8-FITC (#140403, biolegend), CD45-BV605 (#013155, biolegend), CD3-PE-Cy7 (#100219, biolegend), Ly6g-FITC (#127605, biolegend), Gr1-Per/Cy5 (#108427, biolegend) and CD11b-PE (#101207, biolegend).
For T cell toxicity assay, T cells were stimulated with 1 μl of cell activation cocktail (#423303, biolegend) for 4.5 to 6 h at 37 °C. After stimulation, cells were fixed and permeabilized using fix/perm working solution (562574, BD) and intracellular staining permeabilization wash buffer (562574, BD) according to the manufacturer’s protocol. Intracellular staining was performed using aIFN-γ and aGZMB (biolegend) antibodies. Flow cytometry data acquisition was performed using a flow cytometer instrument (CytoFLEX). Flow cytometry data were analyzed using Flowjo (v10.8.1) software.

Shen X.T., Xie S.Z., Zheng X., Zou T.T., Hu B.Y., Xu J., Liu L., Xu Y.F., Wang X.F., Wang H., Wang S., Zhu L., Yu K.K., Zhu W.W., Lu L., Zhang J.B., Chen J.H., Dong Q.Z., Yang L.Y, & Qin L.X. (2024). Cirrhotic-extracellular matrix attenuates aPD-1 treatment response by initiating immunosuppressive neutrophil extracellular traps formation in hepatocellular carcinoma. Experimental Hematology & Oncology, 13, 20.

+ Open protocol

+ Expand

Isolation and Characterization of Tumor-Infiltrating Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor tissue was washed several times in PBS, finely chopped with a razor blade and digested in HBSS containing 1mg/ml Collagenase type IV (Sigma-Aldrich, C5138),100ug/ml DNase I (Sigma-Aldrich, D5319), and 5% fetal calf serum for 10min at 37 ℃ with gentle rotation in shaker (150 rpm). Leukocytes were isolated from the supernatant with Percoll (Solarbio, P8370) gradient separation method in which the cells were responded in 40% Percoll and underlayered with 80% Percoll followed by centrifugation at 2500 rpm for 20min. For surface marker staining, cells were washed with PBS containing 0.5% BSA and stained with CD45-BV605 (103,140, Biolegend), F4/80-APC-cy7 (123,117, Biolegend), CD11b-APC (17-0112-82, eBioscience), CD11c-PE (12-0114-81, eBioscience), CD206-BV421 (141,717, Biolegend), Ly6G-PE-cy7 (127,617, Biolegend), CD3e-PerCP (100,325, Biolegend), CD4-AF700 (100,430, Biolegend) and CD8-FITC (11-0081-82, eBioscience) for 30 min in the dark. Serum levels of Data acquisition were performed on an LSR Fortessa instrument (BD Biosciences) and analyzed by using FlowJo software (Treestar) and SPSS26.0. IL-18 and IFN-γ were detected by enzyme-linked immunosorbent assay (ELISA, MultiScience).

Fan X., Yan Z., Lin Y., Wang Q., Jiang L., Yao X., Dong L., Chen L., Zhao T., Zhao J., Hu H, & Wang H. (2024). Mechanism exploration of Zoledronic acid combined with PD-1 in the treatment of hepatocellular carcinoma. Cancer Immunology, Immunotherapy : CII, 73(4), 62.

+ Open protocol

+ Expand

Isolation and Co-culture of hiMicroglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

After hiMac generation, hiMacs were positively selected by fluorescence-activated cell sorting (FACS, MoFlo Astrios, Beckman Coulter, Villepinte, France) using CD45-BV605 (304042, Biolegend, Paris, France, 1:300), CD11b-BV421 (301324, Biolegend, 1:300), CD14-FITC (982502, Biolegend, 1:300), CD163-APC (333610, Biolegend, 1:300), and CX3CR1-PE (341604, Biolegend, 1:300). FACS-sorted cells were co-cultured with 70–80% confluent three-week-old (day 0 = induction of neural induction) hiNeurons for three weeks. After three weeks of co-culture, cells were used for imaging. To obtain a pure culture of hiMicros for downstream qPCR analysis, FACS-sorted hiMacs were co-cultured for three weeks on 24-well-plates in inserts (353495, Falcon, pore size 0.4 µm) with confluent three-week-old hiNeurons. During co-culture, 50 ng/mL recombinant human CSF-1 (216-MC-010, R & D Systems) and 50 ng/mL human IL-34 (5265-IL-010, R & D Systems) were added every third day to the culture medium.

Feige L., Kozaki T., Dias de Melo G., Guillemot V., Larrous F., Ginhoux F, & Bourhy H. (2022). Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses. Viruses, 15(1), 88.

+ Open protocol

+ Expand

Tumor-Infiltrating Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A systematic analysis of the in vivo induction of antitumor immune responses was performed by harvesting tumors from mice at appropriate time points and preparing a single-cell suspension by digesting these tumors for 2 h at 37 °C using DNAse and Collagenase IV in PBS. These cells were then stained using the Fixable Viability Kit-Zombie Red (Biolegend, Catalog: 423,110) and with the following fluorochrome-conjugated antibodies: CD45-BV605 (Biolegend, Catalog: 103,139), CD3-PerCP-Cy5.5 (Biolegend, Catalog: 100,218), CD4-FITC (Biolegend, Catalog: 100,509), CD8a-BV510 (Biolegend, Catalog: 100,751), CD25-APC (Biolegend, Catalog: 102,012) and Foxp3-PE (Biolegend, Catalog: 320,008). After labeling, cells were analyzed via flow cytometry.

Chen L., Xue W., Cao J., Zhang S., Zeng Y., Ma L., Qian X., Wen Q., Hong Y., Shi Z, & Xu Y. (2022). TiSe2-mediated sonodynamic and checkpoint blockade combined immunotherapy in hypoxic pancreatic cancer. Journal of Nanobiotechnology, 20, 453.

+ Open protocol

+ Expand

Isolation and Characterization of Corpus Callosum Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Focal demyelinated lesions of the corpus callosum of 8-12 week old
C57Bl6J male mice were dissected out and homogenized with a 2 ml dounce. A
Percoll (Sigma-Aldrich) gradient was used to isolate cells from myelin debris.
Samples were blocked with Fc-block [LEAF-purified anti-mouse CD16/32 (Biolegend,
101321)], then incubated with fluorochrome-conjugated antibodies CD11b-PeCy7
(eBioscience, 25-0112-82, 1:100) and CD45-BV605 (Biolegend, 103139, 1:100) for
30 minutes on ice, followed by incubation with ‘FITC Annexin-V apoptosis
detection kit with 7-AAD’ for 15 minutes at room temperature (Biolegend,
640922, 1:20). Following washes in buffer, samples were run on the BD LSR
Fortessa (6 laser) analyser, and analysed using FlowJo version 9/10 software
(FlowJo LLC).

Lloyd A.F., Davies C.L., Holloway R.K., Labrak Y., Ireland G., Carradori D., Dillenburg A., Borger E., Soong D., Richardson J.C., Kuhlmann T., Williams A., Pollard J.W., des Rieux A., Priller J, & Miron V.E. (2019). Central nervous system regeneration is driven by microglia necroptosis and repopulation. Nature neuroscience, 22(7), 1046-1052.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!