Mouse albumin elisa kit

The human pancreatic cancer cell lines Hs766T and Capan1 were obtained from the American Type Culture Collection (ATCC, USA). Hs766T was cultured with Dulbecco’s modified Eagle medium (DMEM; obtained from Hyclone, USA) containing 10% fetal bovine serum (FBS; Hyclone) and 1% penicillin-streptomycin (Thermo Fisher Scientific, USA). Capan1 was cultured with Iscove’s modified Dulbecco medium (IMDM; obtained from Gibco, USA) containing 20% FBS and 1% penicillin-streptomycin. Recombinant human soluble DLL1 was obtained from Enzo Life Sciences (USA). Mouse albumin ELISA kit and mouse alanine aminotransferase ELISA kit were obtained from Abcam (UK), mouse total bilirubin ELISA kit was obtained from Fusheng Industrial Co., Ltd. (China). Antibodies against NICD1 (Cat No. 3608) and NICD3 (Cat No. 2889) were purchased from Cell Signaling Technology (USA). Antibodies against β-actin (Cat No. sc-47778) was obtained from Santa Cruz Biotechnology (USA).

The following reagents were used: transfection was performed with Lipofectamine RNAiMAX Reagent (Invitrogen Life Technologies, 13, 778-030) according to the manufacturer’s protocol. The following antibodies were used: anti-β-actin antibody (Proteintech, 66009-1-Ig); anti-m6A antibody (Synaptic Systems, 202,003); anti-METTL3 antibody (Abcam, Ab195352, ZEN-BIOSCIENCE [382,974]); anti-METTL14 antibody (Cell Signaling Technology, 51, 104); anti-WTAP antibody (Proteintech, 10200-1-AP); anti-KIAA1429 antibody (SAB, 29, 774); anti-TIMP2 antibody (Abcam, ab180630); anti-NF-κB p65 antibody (Cell Signaling Technology, 8242); anti-phospho-NF-κB p65 antibody (Cell Signaling Technology, 3033); anti-cleaved caspase3 antibody (Abcam, Ab2302); anti-NOTCH3 antibody (Proteintech, 55114-1-AP); anti-NOTCH4 antibody (Affinity, DF13597); anti-WT-1 antibody (Abcam, ab267377); anti-nephrin antibody (Abcam, ab216341); anti-podocin antibody (Abcam, ab181143); anti-MMP2 antibody (Proteintech, 10373-2-AP); anti-MT1-MMP antibody (Proteintech, 14552-1-AP); anti-IGF2BP2 antibody (Proteintech, 11601-1-AP); anti-col-Ⅳ antibody (Abcam, ab6586); and anti-fibronectin antibody (Abcam, ab6586). STZ was purchased from Sigma Chemical Company (MO, USA). The PAS kits were obtained from Solarbio (Beijing, China). Mouse Albumin ELISA Kit was obtained from Abcam Biotechnology (Cambridge, UK).

Blood samples were collected from experimental mice after anesthesia. Urine samples were collected every 2 or 3 weeks. Serum samples were detected for albumin by a standard chemical method. Serum and urine creatinine level as well as serum creatinine and blood urea nitrogen (BUN) level were measured by enzymatic method with commercial kits. The mouse albumin ELISA kit (Abcam) was used to detect urine albumin. All measurements were performed in triplicate.