Lipopolysaccharides (lps)

Manufactured by Immunotools

Sourced in Germany

LPS (Lipopolysaccharide) is a complex molecule that is a major component of the outer membrane of Gram-negative bacteria. It plays a crucial role in the structural integrity and function of these bacteria. LPS is a commonly used laboratory reagent for various research applications.

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Lab products found in correlation

Immukin, by Boehringer Ingelheim (1 mentions) Bio plex, by Bio-Rad (1 mentions) Gm csf, by Immunotools (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cxcl12, by Thermo Fisher Scientific (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Deoxyribonuclease 1, by Merck Group (1 mentions) Hematoxylin, by Merck Group (1 mentions) Rt pcr system, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline tablet, by Merck Group (1 mentions) Bovine serum albumine (bsa), by Merck Group (1 mentions) Nkp46 m20, by Santa Cruz Biotechnology (1 mentions) Secondary abs, by Agilent Technologies (1 mentions) Ifn γ, by Immunological Sciences (1 mentions) Penicillin g, by Thermo Fisher Scientific (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Percoll, by Merck Group (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Transwell insert, by BD (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

4 protocols using lipopolysaccharides (lps)

Neutralizing Antibodies to GM-CSF

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GM-CSF–mediated upregulation of LPS-induced production of IL-6 by U937 cells was used to test for the neutralizing activity of sera containing high-titer blocking antibodies to GM-CSF.
Anti–GM-CSF–positive sera, negative control sera, or FBS were preincubated for 30 minutes in a 96-well plate without stimulus or in the presence of GM-CSF (100 ng/mL; ImmunoTools, Friesoythe, Germany), IFN-γ (2 × 10⁴ IU/mL; Immukin; Boehringer Ingelheim, Ingelheim am Rhein, Germany), or LPS (1 µg/mL, BioLabs) alone or in combination as indicated at a dilution of 1:5 in complete Dulbecco modified Eagle medium (Gibco, Carlsbad, Calif). U937 cells (ATCC CRL1593.2) were added at 1 × 10⁵ cells per well and incubated for 24 hours (37°C in a 5% CO₂ atmosphere).
Supernatants were obtained, and IL-6 levels were measured with a Luminex analyzer (R&D Systems Fluorokinemap and Bio-Plex, Bio-Rad Laboratories), according to the manufacturer’s recommendations.

Rosenberg J.M., Price J.V., Barcenas-Morales G., Ceron-Gutierrez L., Davies S., Kumararatne D.S., Döffinger R, & Utz P.J. (2015). Protein microarrays identify disease-specific anti-cytokine autoantibody profiles in the landscape of immunodeficiency. The Journal of allergy and clinical immunology, 137(1), 204-213.e3.

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Isolation and Analysis of Microglia

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All Culture media, fetal bovine serum (FBS), goat serum, penicillin G, streptomycin, glutamine, Na pyruvate, recombinant human EGF, Thermo Script RT‐PCR System, anti‐Acetyl‐alpha Tubulin (6‐11B‐1), anti‐Acetyl‐alpha Tubulin lys40 Abs and Hoechst (#33342, RRID:AB_10626776) were from GIBCO Invitrogen (Carlsbad, CA). Glucose, hematoxylin, eosin, Percoll, Papain (#P3125), phosphate buffered‐saline (PBS) tablet (#P4417), Bovine Serum Albumine (BSA) and deoxyribonuclease I were from Sigma‐Aldrich (Milan, Italy). Transwell inserts were from BD Labware (Franklin Lakes, NJ). Ki67 (#12202, RRID:AB 2620142) Ab were from Cell Signaling (Danvers, MA). NKp46 (M20) (#sc‐18,161, RRID: AB_2149152) antibody (Ab) was from Santa Cruz biotechnology (Santa Cruz, CA). Anti‐alpha tubulin (ab52866) and anti‐TMEM119 Abs were from Abcam (Cambridge, UK). Secondary Abs were from DAKO (Milan, Italy). CXCL12 was from Peprotech. LPS is from Immunotools (Friesoythe, Germany). IFN‐γ were from Immunological Sciences (Rome, Italy). Microbeads CD11b⁺ were from Miltenyi Biotec (Bologna, Italy). CD45, CD69, CD107a, NK1.1 Abs were from eBioscience Inc. (San Diego, CA). Rabbit anti‐Iba1 from Wako (VA).

Mormino A., Cocozza G., Fontemaggi G., Valente S., Esposito V., Santoro A., Bernardini G., Santoni A., Fazi F., Mai A., Limatola C, & Garofalo S. (2021). Histone‐deacetylase 8 drives the immune response and the growth of glioma. Glia, 69(11), 2682-2698.

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Trophoblastic Cell Responses to Inflammatory Stimuli

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Trophoblastic cell lines (4 × 10⁵ HTR-8/SVneo and JEG-3 per well) were seeded in 6-well culture plates and allowed to attach overnight. Cells were stimulated with 100 ng/mL LPS, 25 μg/mL poly I:C (both from Sigma-Aldrich) or medium alone as control for 6, 12 and 24 h. Based on the results from cell lines, PTC were incubated for 12 h with the same concentrations of LPS and poly I:C.
For the assessment of angiogenic factors and miRNAs, 2 × 10⁵ HTR-8/SVneo, PTC or HUVEC cells were cultured in 12-well plates and then treated for 24 h with 20 or 50 ng/mL of IL-36 (α, β, or γ; ImmunoTools GmbH, Friesoythe, Germany).
After stimulation, cells were harvested for RNA isolation or Western blotting.

Murrieta-Coxca J.M., Gutiérrez-Samudio R.N., El-Shorafa H.M., Groten T., Rodríguez-Martínez S., Cancino-Diaz M.E., Cancino-Diaz J.C., Favaro R.R., Markert U.R, & Morales-Prieto D.M. (2020). Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells. International Journal of Molecular Sciences, 22(1), 285.

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Molecular Regulatory Mechanisms in Cell Signaling

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General laboratory chemicals were obtained from Sigma-Aldrich or Merck Millipore (Darmstadt, Germany). TNF-α and TRAIL were purchased from PeproTech Inc (Rocky Hill, NJ, USA). Pam3CSK4, LPS and R848 were purchased from ImmunoTools (Friesoythe, Germany). Anti-β-ACTIN antibody was bought from Sigma-Aldrich. Anti-p53 and anti-COP1 antibodies were obtained from Santa Cruz Biotechnology Ltd (Dallas, Texas, USA). Anti-SHH, anti-GLI1, anti-PTCH1, anti-NUMB, anti-Ser9 phospho GSK-3β and anti-UbK48 were purchased from Cell Signaling Technology (Danvers, MA, USA). Annexin V- fluorescein isothiocyanate (FITC) was from Miltenyi Biotech (Bergisch Gladbach, Germany). HRP conjugated anti-rabbit IgG and anti-mouse IgG were obtained from Jackson ImmunoResearch (West Grove, PA, USA).

Holla S., Ghorpade D.S., Singh V., Bansal K, & Balaji K.N. (2014). Mycobacterium bovis BCG promotes tumor cell survival from tumor necrosis factor-α-induced apoptosis. Molecular Cancer, 13, 210.

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