V 730 bio

Manufactured by Jasco

Sourced in Japan

The V-730 BIO is a UV-Vis spectrophotometer designed for a variety of applications in biological research and analysis. It measures the absorbance or transmittance of light passing through a sample over a range of ultraviolet and visible wavelengths.

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Lab products found in correlation

Em 002b, by Topcon (4 mentions) Tecnai tf 20 feg tem, by Thermo Fisher Scientific (1 mentions) Quantax eds system, by Bruker (1 mentions) Spectrum one, by PerkinElmer (1 mentions) Uv 2600, by Shimadzu (1 mentions) Fa200, by JEOL (1 mentions) Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Rf 5300, by Shimadzu (1 mentions) Bioruptor ucd 250, by Cosmo Bio (1 mentions)

13 protocols using v 730 bio

Characterization of LM Nanocapsules

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The structure and morphology of the prepared LM nanocapsules were observed using
high-resolution TEM (EM-002B; Topcon, Tokyo, Japan) with an accelerating voltage
of 120 kV. A small droplet of sample placed on a grid or
10 μl of sample solution (LM concentration:
100 μg ml⁻¹) were
irradiated using a fibre-coupled CW laser at 785 nm for
3 min (spot diameter, ∼4 mm; maximum power:
1 W,
∼80 mW mm⁻²;
BRM-785-1.0-100-0.22-SMA; B&W Tek, Newark, DE, USA). The polymer shell
structure of nanocapsules and STEM/EDS mapping were performed by Nanoscience Co.
in Evans Analytical Group Company, Inc. (Tokyo, Japan). The samples were imaged
with a FEI Tecnai TF-20 FEG/TEM operated at 200 kV in bright-field
TEM mode, high-resolution (HR) TEM mode, and high-angle annular dark-field
(HAADF) STEM mode. The STEM probe size was 1–2 nm nominal
diameter. EDS mapping were acquired on Oxford INCA, Bruker Quantax EDS
system.
The hydrodynamic diameter of LM nanocapsules was examined by DLS (Photal
FPAR-1,000; Otsuka Electronics, Osaka, Japan). DLS diagram of laser-induced LM
nanocapsules was also measured. A 100 μl of sample solution
(LM concentration:
100 μg ml⁻¹) was
irradiated using a fibre-coupled CW laser (maximum power: 1 W,
∼80 mW mm⁻²) at
785 nm for 1 h before DLS measurements.
The concentration of LM and carmofur in nanocapsules was estimated with a
ultraviolet–visible–NIR spectrophotometer (V-730 BIO; Jasco,
Tokyo, Japan).

Chechetka S.A., Yu Y., Zhen X., Pramanik M., Pu K, & Miyako E. (2017). Light-driven liquid metal nanotransformers for biomedical theranostics. Nature Communications, 8, 15432.

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Spectroscopic Analysis of Bacterial ICG Uptake

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Absorption spectra of bacterial solutions were recorded at 20 °C using a UV–vis–NIR spectrophotometer (V‐730 BIO; Jasco, Tokyo, Japan). The fluorescence of bacterial suspensions was measured using a fluorescence spectrometer (FP‐8600 NIR Spectrofluorometer; Jasco, Tokyo, Japan). The amount of loaded and internalized ICG into bacteria was calculated from the collected supernatant of washed i‐PM after modification of ICG‒CRE by a UV–vis–NIR spectrophotometer.

Goto Y., Iwata S., Miyahara M, & Miyako E. (2023). Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics. Advanced Science, 10(20), 2301679.

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Characterization of Nanodiamonds with Camptothecin

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The structure and morphology of the prepared ND-SPs were visualized using a high-resolution transmission electron microscope (TEM) (EM-002B, Topcon, Tokyo, Japan) at an accelerating voltage of 120 kV.
The hydrodynamic diameter of the ND-SPs was determined via dynamic light scattering (DLS) (Photal FPAR-1000, Otsuka Electronics, Osaka, Japan).
A UV-Vis-NIR spectrophotometer (V-730 BIO, Jasco, Tokyo, Japan) was used to measure the spectral profiles and concentrations of the ND-SPs and CPT complexes.
Fourier transform infrared (FTIR) spectroscopy (Spectrum One, PerkinElmer, Yokohama, Japan) analysis was carried out to identify the presence of alkyl chains on the ND surface.

Yu Y., Yang X., Liu M., Nishikawa M., Tei T, & Miyako E. (2019). Anticancer drug delivery to cancer cells using alkyl amine-functionalized nanodiamond supraparticles. Nanoscale Advances, 1(9), 3406-3412.

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Photoautotrophic Growth of Synechocystis

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Synechocystis cells were cultured in 1,000 ml Erlenmeyer flasks containing 500 ml of the BG11 medium (Stanier et al., 1971 (link)) for photoautotrophic growth at 30°C under continuous light (15 μmol photons m^–2 s^–1) with rotary shaking at 100 rpm. For the N-starved growth, cells were first grown in BG11 medium for 7 days and collected by centrifugation (1,840 × g, 10 min). After a wash of the cell precipitates with N-free medium (BG11₀: BG11 without nitrate), cells were resuspended in BG11₀ to set OD₇₃₀ = 0.4. For the recovery growth from N starvation, cells grown in BG11₀ for 7 days were harvested (1,840 × g, 10 min) and resuspended in the BG11 medium to set OD₇₃₀ = 0.6. The cell density was measured using a spectrophotometer (UV-2600, Shimadzu, Japan or V730BIO, JASCO, Japan). For PG supplementation, 40 mM dioleoyl-PG (Sigma-Aldrich) dissolved in 100% ethanol was added to growth media at a final concentration of 20 μM PG and 0.05% (v/v) ethanol. As the negative control of PG supplementation, 100% ethanol was added to growth media at a final concentration of 0.05% (v/v).

Kobayashi K., Osawa Y., Yoshihara A., Shimojima M, & Awai K. (2020). Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803. Frontiers in Plant Science, 11, 432.

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Quantification of Reactive Oxygen Species

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·OH and ·O₂⁻ radicals were captured using DMPO as a spin‐trapping agent and measured by ESR. In a typical procedure for ·OH detection, samples (5 mg) were dispersed in Ar‐saturated H₂O (2.5 mL). Then aqueous DMPO solution (30 µL, 2.0 m) was added to the sample suspension, followed by vigorous shaking for 10 s. The mixture was irradiated either by light, ultrasound, or the combined light and ultrasound for 30 s and then analyzed by ESR (JEOL FA200). In a typical procedure for ·O₂⁻ detection, samples (5 mg) were dispersed in oxygen‐saturated methanol (2.5 mL). Then methanolic DMPO solution (50 µL, 0.2 m) was added to the sample suspension, followed by vigorous shaking for 10 s. The mixture was irradiated either by light, ultrasound, or the combined light and ultrasound for 30 s and then analyzed by ESR (JEOL FA200). The ·O₂⁻ production was also monitored by NBT degradation. In a typical procedure, samples (2 mg) were dispersed in water (5 mL), followed by the addition of an aqueous NBT solution (125 µL, 1 × 10⁻³m). The resulting mixture was stirred in the dark for 10 min. Subsequently, a xenon lamp was used as the light source to irradiate the mixture. After a certain time (10 min), 1 mL of the mixture was withdrawn and filtered using a 0.2 µm PTFE filter. The absorption of the filtrate at 259 nm was monitored by a UV–Vis spectrophotometer (Jasco V‐730 BIO).

Jiang W., Zhu H., Yang J., Low B.Q., Wu W., Chen M., Ma J., Long R., Low J., Zhu H., Heng J.Z., Tang K.Y., Chai C.H., Lin M., Zhu Q., Zhang Y., Chi D., Li Z., Loh X.J., Xiong Y, & Ye E. (2023). Integration of Single‐Atom Catalyst with Z‐Scheme Heterojunction for Cascade Charge Transfer Enabling Highly Efficient Piezo‐Photocatalysis. Advanced Science, 10(28), 2303448.

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Characterization of Nanocomplex Formulations

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The spectral profiles and concentrations of loaded i-Extract in nanocomplexes were measured with a UV-Vis-NIR spectrophotometer (V-730 BIO; Jasco, Tokyo, Japan). The structure and morphology of prepared FRi-ExNCs were visualized by high-resolution transmission electron microscope (TEM) (EM-002B; Topcon, Tokyo, Japan), an established and suitable method for the investigation of structural aspects of nanomaterials, at an accelerating voltage of 120 kV. Before the TEM experiments, a nanocomplex solution was deposited on a carbon-coated support and negatively stained using a 1% phosphotungstic acid solution. The size distribution was analyzed using Image-J from TEM images (≥200 particles were measured).

Yu Y., Wang J., Kaul S.C., Wadhwa R, & Miyako E. (2019). Folic Acid Receptor-Mediated Targeting Enhances the Cytotoxicity, Efficacy, and Selectivity of Withania somnifera Leaf Extract: In vitro and in vivo Evidence. Frontiers in Oncology, 9, 602.

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Characterizing TRPV2-PCNH Complexes

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Absorption spectra of TRPV2–PCNH solutions were recorded at room temperature using a UV–Vis–NIR spectrophotometer (V-730 BIO; Jasco, Tokyo, Japan). Hydrodynamic diameters of the PCNH and TRPV2–PCNH complexes were determined using DLS (Photal FPAR-1000; Otsuka Electronics, Osaka, Japan). Negative staining was used to observe morphology and structure of TRPV2–PCNH molecules with a high-resolution TEM (EM-002B; Topcon, Tokyo, Japan) at an acceleration voltage of 80 kV.

Yu Y., Yang X., Reghu S., Kaul S.C., Wadhwa R, & Miyako E. (2020). Photothermogenetic inhibition of cancer stemness by near-infrared-light-activatable nanocomplexes. Nature Communications, 11, 4117.

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Monitoring Protein-Ligand Interactions

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Several techniques have been developed in an effort to try and better understand protein–protein and protein–ligand interaction, as the basis of protein interaction opens the doorway to understanding vital reactions in organisms [14 (link)]. The study of proteins binding to ligands requires that we study and understand how proteins interact with drugs in terms of their conformation, binding affinities, kinetics and thermodynamics, in an effort to develop specific drugs that target proteins that have a vital role in the diseases and illness [7 (link),14 (link)]. UV-Vis spectrophotometry can be used to monitor protein secondary structure changes through three aromatic amino acids: Phe, Tyr and Trp. The conformational changes induced by the inhibitors were monitored by UV–Vis–NIR spectrophotometer (V-730 BIO; Jasco, Tokyo, Japan). Vials containing 20 µg/mL of PfHGXPRT were, respectively, exposed to 0, 5 and 10 mM concentrations of iso-mukaadial acetate and ursolic acid acetate for 15 min. PfHGXPRT was similarly exposed to the commercial standard inhibitor, Triethyl-2-phosphonobutyrate (Tri). The PfHGXPRT spectra were collected at room temperature before and after the addition of all inhibitors.

Opoku F., Govender P.P., Pooe O.J, & Simelane M.B. (2019). Evaluating Iso-Mukaadial Acetate and Ursolic Acid Acetate as Plasmodium falciparum Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase Inhibitors. Biomolecules, 9(12), 861.

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Spectrophotometric Determination of Enzyme Kinetics

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Enzyme activities were determined by spectrophotometry (V-730 BIO, JASCO, Tokyo, Japan) at 303 K in 20 mM HEPES buffer (pH 7.0) with 50 μM ZnSO₄. The wavelengths and extinction coefficients used in this study were the same as those reported by Laraki et al. (26 (link)). Protein concentrations were determined using a BCA Protein Assay Kit (PIERCE, Illinois, USA) and bovine serum albumin as the standard. The enzyme was diluted with the assay buffer containing 20 μg mL⁻¹ bovine serum albumin (BSA) to prevent denaturation. The values of the kinetic parameters (K_m and k_cat) were obtained by a double-reciprocal (Lineweaver-Burk) plot of initial steady-state velocities at different substrate concentrations (38 ).

Yamamoto K., Tanaka H., Kurisu G., Nakano R., Yano H, & Sakai H. (2022). Structural insights into the substrate specificity of IMP-6 and IMP-1 metallo-β-lactamases. Journal of Biochemistry, 173(1), 21-30.

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Indigoidine Extraction and Quantification

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Fresh fungal mycelia (50 mg) were ground with liquid nitrogen and then subjected to extraction of indigoidine by adding 1 mL of dimethyl sulfoxide (DMSO) with sonication, as previously described [18 ]. For the extracellular indigoidine extraction, the cell-free broth was diluted with 100% (v/v) DMSO solution. The prepared samples were subjected to quantification of indigoidine using a spectrophotometer (Jasco V-730BIO) at a wavelength of 612 nm. A calibration curve for calculating pigment concentration was generated using an indigoidine standard with known concentrations (ViabLife, PRC).
To analyze the indigoidine pigment, 10 μL of the sample dissolved in DMSO was subjected to reversed-phase high-performance liquid chromatography (RP-HPLC) column (Ultimate 3000, Thermo, USA) equipped with a diode-array detector (DAD) and C-18 Acclaim^™120 column (4.6× 250 mm, 5-mm particle size, Bio-Rad Laboratories, USA). The gradient system was set up according to a previous report [32 (link)], with a slight modification to methanol concentration gradient. The pigment was monitored at 600 nm with a running time of 20 min and then compared with the standard.

Panchanawaporn S., Chutrakul C., Jeennor S., Anantayanon J., Rattanaphan N, & Laoteng K. (2022). Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity. PLoS ONE, 17(6), e0270359.

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