Water samples (500 mL) destined for MPP analysis were fixed with Lugol’s iodine solution to a final dilution of 1.0%, stored at 4 °C, and processed within four weeks. Identification and counting were carried out under an inverted microscope (Labovert FS Leitz equipped with phase contrast), equipped with an AXIOCAM Icc 5 digital camera (Carl Zeiss, Oberkochen, Germany), following the Utermöhl method [38 ]. According to the observed MPP abundances, a variable volume of the sample (50–100 mL) was settled in an Utermöhl chamber. The minimum value of the counted cells was 200 cells per sample for a confidence limit of 14% [39 ]. Microalgal cell sizes were measured using the AXIOCAM Icc 5 digital camera (Carl Zeiss, Oberkochen, Germany). The biovolume was calculated by assigning to each cell one geometrical body, or, in some cases, a combination of more geometrical bodies, and applying standard formulae according to Hillebrand et al. [40 (link)]. The obtained biovolumes were converted to carbon content using the conversion factors introduced by Menden-Deuer and Lessard [41 (link)].
Axiocam icc 5 digital camera
Manufactured by Zeiss
Sourced in Germany
The AXIOCAM Icc 5 is a digital camera designed for microscopy applications. It features a high-resolution sensor and can capture detailed images of specimens under observation.
Automatically generated - may contain errors
Lab products found in correlation
Axiovert 200m, by Zeiss (2 mentions)
Lumar v12 stereomicroscope, by Zeiss (2 mentions)
Eos 5d mark 2, by Canon (1 mentions)
Axio lab a1 microscope, by Zeiss (1 mentions)
Ascorbic acid, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Alexa fluor 647, by Zeiss (1 mentions)
Alexa fluor 568, by Zeiss (1 mentions)
Alexa fluor 488, by Zeiss (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Axio vert a1 microscope, by Zeiss (1 mentions)
Permount mounting medium, by Thermo Fisher Scientific (1 mentions)
Digital camera, by Nikon (1 mentions)
Imager d2 microscope, by Zeiss (1 mentions)
7 protocols using axiocam icc 5 digital camera
1
Microplankton Biovolume and Carbon Content
2
Imaging Analysis of Rice Reproductive Development
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Whole plants and panicles following seed maturation of the wild type and osmads58 were photographed with a Nikon digital camera. Florets were photographed using a ZEISS Lumar V12 stereomicroscope (Carl Zeiss, Oberkochen, Germany). Mature pollen grains were stained with I2-KI solution for a few minutes and then visualized using an Imager D2 microscope and a ZEISS AxioCam ICc5 digital camera (Carl Zeiss, Oberkochen, Germany).
For semi-thin sectioning, panicles were fixed in FAA fixative (50% ethanol, 10% formaldehyde, 5% acetic acid) overnight at 4 °C, and then the materials were dehydrated under a gradient series of ethanol (50%, 70%, 80%, 95%, and 100%). The panicles were transferred to 1:1 ethanol:LR white resin (v/v) overnight and then transferred to 100% LR white resin for 24 h. The samples polymerized for 24 h at 65 °C. The embedded samples were sectioned (4 μm thick) before staining with toluidine blue O (Urchem, Shanghai, China) and imaged using an Imager.D2 microscope with a ZEISS AxioCam ICc5 digital camera (Carl Zeiss, Oberkochen, Germany).
For semi-thin sectioning, panicles were fixed in FAA fixative (50% ethanol, 10% formaldehyde, 5% acetic acid) overnight at 4 °C, and then the materials were dehydrated under a gradient series of ethanol (50%, 70%, 80%, 95%, and 100%). The panicles were transferred to 1:1 ethanol:LR white resin (v/v) overnight and then transferred to 100% LR white resin for 24 h. The samples polymerized for 24 h at 65 °C. The embedded samples were sectioned (4 μm thick) before staining with toluidine blue O (Urchem, Shanghai, China) and imaged using an Imager.D2 microscope with a ZEISS AxioCam ICc5 digital camera (Carl Zeiss, Oberkochen, Germany).
3
Documentation of Osmads58 Floral Morphology
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Whole plants and panicles following seed maturation of the wild type and osmads58 were photographed with a Nikon digital camera. Florets were photographed using a ZEISS Lumar V12 stereomicroscope (Carl Zeiss). Mature pollen grains were stained with I 2 -KI solution for a few minutes and then visualized using an Imager D2 microscope and a ZEISS AxioCam ICc5 digital camera (Carl Zeiss).
For semi-thin sectioning, panicles were fixed in FAA fixative (50% ethanol, 10% formaldehyde, 5% acetic acid) overnight at 4℃, and then the materials were dehydrated under a gradient series of ethanol (50%, 70%, 80%, 95%, and 100%). The panicles were transferred to 1:1 ethanol:LR white resin (v/v) overnight and then transferred to 100% LR white resin for 24 h. The samples polymerized for 24 h at 65°C. The embedded samples were sectioned (4 μm thick) before staining with toluidine blue O (Urchem) and imaged using an Imager.D2 microscope with a ZEISS AxioCam ICc5 digital camera (Carl Zeiss).
For semi-thin sectioning, panicles were fixed in FAA fixative (50% ethanol, 10% formaldehyde, 5% acetic acid) overnight at 4℃, and then the materials were dehydrated under a gradient series of ethanol (50%, 70%, 80%, 95%, and 100%). The panicles were transferred to 1:1 ethanol:LR white resin (v/v) overnight and then transferred to 100% LR white resin for 24 h. The samples polymerized for 24 h at 65°C. The embedded samples were sectioned (4 μm thick) before staining with toluidine blue O (Urchem) and imaged using an Imager.D2 microscope with a ZEISS AxioCam ICc5 digital camera (Carl Zeiss).
4
Isolation and Identification of Endophytic Fungus from Kalmia angustifolia
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The endophytic fungus SWUKD3.1410 used in this study was isolated from fresh, healthy branches of K. angustifolia collected in November (dry season) 2012 at Xichou (N23°26′18.10″, E104°40′19.92″) of Yunnan province of China, lying approximately 55.8 km apart (Huang et al., 2015 (link)). The strain was preliminarily identified based on morphological characterization after 7 days in a PDA (potato 200 g, glucose 20 g, agar 18 g, and water 1000 mL) medium. To stimulate sporulation, the strain was cultured in the water-agar plates and placed in darkness at 28°C for up to 20 days. For morphological studies, slide cultures were prepared and stained with lactophenol cotton blue dye. Using a Canon EOS 5D Mark II digital camera, morphology of colonies (texture, color, and presence of pigmentation) grown on PDA media was photographed as described in Simoes et al. (2013) (link). The microscopic characteristics (morphology of vegetative spores’ structures) were observed with an Axio Lab. A1 microscope (Carl Zeiss, Germany) equipped with an Axiocam ICc 5 digital camera (Carl Zeiss Vision, Germany). Standard taxonomic manuals were used to identify the fungal genus and species (Mahoney et al., 2004 (link); Wang et al., 2013 (link)).
5
MC3T3-E1 Cells Transduction and Protein Analysis
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MC3T3‐E1 sc4 (referred to as MC3T3 or MC3T3‐E1) cells were maintained in αMEM with no ascorbic acid (Gibco/Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco/Thermo Fisher Scientific). Cells were seeded in 12‐well plates at a density of 10,000 cells/cm2 and allowed to adhere overnight. The next day, cultures were transduced with Ad‐GFP‐Cre in the presence of vehicle (DMSO) and several +JQ1 (Cayman Chemical, Ann Arbor, MI, USA) concentrations. Four days later, GFP fluorescent images were captured on an Axio Vert.A1 microscope using an AxioCam ICc 5 digital camera (Zeiss, Thornwood, NY, USA) and cells were then lysed and protein levels assessed by Western blotting analysis.
6
Fluorescence Microscopy of Stressed Cells
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Standard procedure Cells were fixed and processed for fluorescence microscopy as described previously 81 . Briefly, cells were grown on glass coverslips, stressed as indicated, fixed using 3.7 % formaldehyde (Sigma) in PBS for 10 min, followed by 5 min post-fixation/permeabilization in ice-cold methanol. Cells were blocked for 1 h in 5% horse serum/PBS, and primary and secondary incubations performed in blocking buffer for 1 h. All secondary antibodies were raised in donkey against either mouse, rabbit or goat and tagged with either Alexa Fluor 488, 568 or 647 (Thermo Fisher). Following washes with PBS, cells were mounted in polyvinyl mounting media and viewed using a Zeiss Axiovert 200M with a 63X Plan Apochromat objective lens (NA 1.4) and illuminated with a mercury lamp and standard filters for DAPI (Filter Set 49: 335-383 / 395 / 420-470), Alexa Fluor 488 (Filter Set 10: 450-490 / 510 / 515-565), Alexa Fluor 568 (Filter Set 20: 540-552 / 560 / 575-640), Alexa Fluor 647 (Filter Set 50: 640/30 / 660 / 690/50). Images were captured using an AxioCam ICc5 digital camera (Zeiss) with the manufacturer's software, and raw TIF files were imported into Adobe Photoshop. Identical adjustments in brightness and contrast were applied to all images in each experiment.
7
Picrosirius Red Staining for Collagen
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One FFPE tissue section from each patient was utilized for picrosirius red staining for collagen. Briefly, the slides were treated with xylenes, degrading alcohol dilutions (100, 95, 70 and 50%), deionized water, and then rehydrated with PBS. The Picrosirius Red solution was applied to completely cover the tissue sections for 60 min (Abcam, Cambridge, U.K., cat. # ab150681). Slides were then rinsed twice with acetic acid, and twice with ethanol. Tissue sections were then mounted with Permount mounting medium (Fisher Scientific, Waltham, MA cat. # SP15-100). After 24 h, all slides were imaged at 20X magnification on a Zeiss Axio Vert.A1 microscope with a Zeiss AxioCam ICc 5 digital camera (Zeiss, Oberkochen, Germany).
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