B6.129 tnfrsf1atm1mak j

All the experimental procedures were approved by and performed in accordance with the guidelines and practices established by the Tulane University Animal Care and Use Committee.
Male mice in which the TNF‐α receptors TNFR1 (B6.129‐Tnfrsf1atm1Mak/J) and TNFR2 (B6.129S2‐Tnfrsf1btm1Mwm/J) had been genetically knocked out and their background WT (WT; C57BL/6) mice were used in these experiments (Jackson Laboratories). All these mice (n = 6 in each group; 9–10 week old with average body weights, WT, 25.7 ± 0.7 g; TNFR1KO, 24.2 ± 0.8 g; TNFR2KO, 24.9 ± 0.6 g) were purchased from Jackson Laboratories. Male mice were only used in this study as we have conducted similar acute experiments in our earlier studies which give us the advantage to compare these results with findings from other similar studies. (Castillo et al., 2012 (link); Shahid et al., 2008 (link)). These mice were housed in a temperature‐ and light‐controlled room in the Tulane Vivarium and allowed free access to standard diet (Ralston‐Purina) and tap water for ≥3 days before acute renal clearance studies were performed under anesthesia.

All animal studies were conducted according to an appropriate license under the Animals (Scientific Procedures) Act of 1986. HOIP‐floxed (Hoip^flox) mice were created from the C57BL/6 embryonic stem cell clone EPD0161_5_A05 from KOMP Repository (http://www.komp.org) and generated by the Wellcome Trust Sanger Institute (Hinxton, UK).20Hoip^flox mice were subsequently crossed to albumin promoter–driven Cre recombinase (Alb‐Cre) mice (B6.Cg‐Tg[Alb‐cre]21Mgn/J) purchased from Jackson Laboratories (Bar Harbor, ME) to delete HOIP in LPCs. TNFR1‐deficient mice (B6.129‐Tnfrsf1atm1Mak/J) and cluster of differentiation 95 (CD95) death domain (DD)–floxed mice (C57BL/6‐Fastm1Cgn/J) were purchased from Jackson Laboratories. Mice were maintained at Charles River (Margate, UK) and UCL Biological Service Unit (London, UK) under specific pathogen‐free conditions and a 12‐hour dark/light cycle and fed ad libitum. Both males and females were used in the experiments.

C57BL/6 (H-2^b), TNFR1^−/− (B6.129-Tnfrsf1a^tm1Mak/J) and TNFR2^−/− (B6.129S2-Tn frsf1b^tm1Mwm/J) mice were purchased from the Jackson Laboratory. Mice were housed in specific pathogen-free conditions at the First Hospital Animal Center of Jilin University. Mice at 6–8 weeks of age were used in experiments. All animal experiments were approved by the Animal Ethical Committee of First Hospital of Jilin University.
B16 and B16-OVA melanoma cells were purchased from ATCC (Rockville, MD). Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), 100 U/mL penicillin and 100 mg/mL streptomycin (both from Invitrogen).