HPLC-fluorescence detection was performed on a PerkinElmer® Flexar® HPLC system (PerkinElmer Flexar®, Waltham, USA) with a quaternary analytical pump, variable wavelength detector, auto-injector sampler, and Chromera® Chromatography Data Systems (CDS) software. The emission and excitation wavelengths of the fluorescence detector were λex = 426 nm and λem = 539 nm for the determination of curcuminoid concentration in the adsorption capacity study. All analyses were carried out on Brownlee Analytical C18 column (150 mm × 6 mm, 5 µm; PerkinElmer) operated at a temperature of 30 °C. The mobile phase was an isocratic elution consisting of acetonitrile and 0.1% TCA (40:60, v/v). The flow rate was maintained at 1.5 mL/min, and the sample injection volume was set to 5 μL. A Soxhlet apparatus was used for template removal from the synthesized MIPs. For spectroscopic characterization, a Thermo Scientific (Nicolet IS5) FT-IR spectroscopy was employed. The surface morphology was analyzed using scanning electron microscopy (SEM) with an electron microprobe JXA 840 A (Jeol, Japan) combined with the LINK analytical system.
Brownlee analytical c18 column
Manufactured by PerkinElmer
Sourced in United States
The Brownlee Analytical C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a variety of chemical compounds. The column features a stationary phase of chemically bonded octadecylsilane (C18) particles, which provide excellent retention and selectivity for a wide range of organic molecules. The column dimensions and packing material are optimized for analytical-scale HPLC applications.
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Lab products found in correlation
Flexar hplc system, by PerkinElmer (1 mentions)
Jxa 840a, by JEOL (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Gadph, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Series 200 hplc system, by PerkinElmer (1 mentions)
3 protocols using brownlee analytical c18 column
1
Curcuminoid Quantification Using HPLC-Fluorescence
2
Cultivation and Characterization of Morel Mushroom
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Fruiting bodies of Morchella importuna were obtained from fields owned by the Sichuan Academy of Agricultural Sciences (SAAS). Molecular sequences generated from the fruiting bodies (no. Cyl-158) were deposited at GenBank (http://www.ncbi.nlm.nih.gov ) under accession numbers MG121861–MG121865. The fruiting bodies were dried in a 37°C oven. The Sephadex 30 increase column was obtained from GE Healthcare (Uppsala, Sweden). The Brownlee analytical C18 column was bought from Perkin Elmer (Shelton, USA).
The human cervical cancer HeLa cell line was acquired from the West China Medical College, Sichuan University. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and trypsin-EDTA were from Gibco (Grand Island, NY, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO). Kits utilized to determine caspase activity (caspase-3 and -9) were obtained from Beyotime (Shanghai, China). Antibodies for Bax, Bcl-2, caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, and GADPH were from Cell Signaling Technology (Danvers, MA, USA). Other chemical reagents involved in this research were of analytical grade.
The human cervical cancer HeLa cell line was acquired from the West China Medical College, Sichuan University. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and trypsin-EDTA were from Gibco (Grand Island, NY, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO). Kits utilized to determine caspase activity (caspase-3 and -9) were obtained from Beyotime (Shanghai, China). Antibodies for Bax, Bcl-2, caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, and GADPH were from Cell Signaling Technology (Danvers, MA, USA). Other chemical reagents involved in this research were of analytical grade.
3
HPLC Profiling of Foliar Phenols
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Phenolic compositions were obtained from a gradient method previously described (Campos and Markham, 2007) (link), with a Perkin Elmer Series 200 HPLC system and a Perkin Elmer Brownlee Analytical C18 column (4.6 × 250 mm, 5 µm). Water adjusted to pH 2.5 with orthophosphoric acid was solvent A and acetonitrile was solvent B. Solvents were mixed according to the following gradient: starting with 100% A, decreasing to 91% over the next 12 min, to 87% over the next 8 min, and to 67% over the next 12 min, and to 57% until the end of the 60 min analysis. Chromatograms were registered at 260 nm. Fifty microliters of samples were injected. The flow rate used was 0.8 mL min -1 . The analyses were carried out at 25°C. Spectral data for all peaks were accumulated in the range of 200 to 400 nm using diode-array detection (Perkin Elmer Series 200). Structural information was obtained by comparisons of retention times (RT) and UV spectra of resolved compounds with those of reference compounds (apigenin: RT: 59.62, λ max : 269, 335; chlorogenic acid: RT: 28.16, λ max : 243sh, 293sh, 325), as well as with the principles of the UV theory developed by Campos and Markham (2007) (link) for flavonoids and phenolic acids. The foliar phenol profile of each species was made up of all compounds present in the respective HPLC-DAD chromatogram, treating each compound as a single chemical character.
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