Thermo Fisher centrifuge (Thermo Fisher Science, USA); VORTEX-GENIE 2 vortex oscillator (SITM, USA); BX7200HP ultrasonic cleaner (Shanghai Xinmiao medical device manufacturing Co., Ltd.); AL104 balance with accuracy of 0.0001 (Mettler Toledo Shanghai Co., Ltd., Switzerland); CF16RN centrifuge (Hitachi, Japan); Ultrapure water meter (MilliPore, USA); and -80 °C ultra-low temperature refrigerator (Thermo Fisher science, USA).
80 c ultra low temperature refrigerator
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China
The −80°C ultra-low temperature refrigerator is a laboratory equipment designed to maintain a consistent temperature of -80°C. It is used for the storage and preservation of materials that require extremely low temperatures, such as biological samples, chemicals, and certain types of research specimens.
Automatically generated - may contain errors
Lab products found in correlation
Ultrapure water meter, by Merck Group (1 mentions)
Enzyme labeling instrument, by Agilent Technologies (1 mentions)
Restriction endonuclease, by Takara Bio (1 mentions)
Dna marker, by Takara Bio (1 mentions)
Micropipette, by Eppendorf (1 mentions)
Milli q ultrapure water machine, by Merck Group (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Moorldi2 hir, by Moor Instruments (1 mentions)
5 protocols using 80 c ultra low temperature refrigerator
1
Comprehensive Experimental Equipment Setup
2
Comprehensive Biological Sample Processing
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The DNA extraction kit and protease K were purchased from Tiangen Biochemistry Technology Co., Ltd., and the crystal violet and the phosphate buffer solution (PBS) buffer were purchased from Shanghai Shenggong Bioengineering Co., Ltd. The PCR polymerase, restriction endonuclease, and DNA marker were purchased from TaKaRa Company of Japan, and the MALDI-TOFMS mass spectrometer was purchased from Bruker Daltonik Company (Germany). The PCR instrument was purchased from the Applied Biosystems Company of the United States, and the enzyme labeling instrument was purchased from the Biotek Company of the United States. The pulsed gel electrophoresis system was purchased from Bio-Rad Company, the Ultra Micro Spectrophotometer (NanoDrop2000) was purchased from Thermo Company, and the Ultraviolet Gel Imager was purchased from Shanghai Tianneng Company. The −80°C ultra-low temperature refrigerator and incubator were purchased from Thermo Company, while the micro-pipette and centrifuge were purchased from Eppendorf Company (Germany).
3
Dried Radix of Panax chinensis Protocol
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Dried Radix of P. chinensis was purchased from a Chinese herbal medicine store in Suzhou and identified by Prof. Xiaoran Li of the School of pharmacy of Suzhou University. The voucher specimen (No. 08-02-15-18) has been deposited at Soochow University. The other materials used in the study were bought from different companies as follows; Salicylazosulfapyridine (SASP) from (Shanghai Xinyi Tianping Pharmaceutical Co., Ltd., gyzz H31020557); CMC-Na (Carboxymethylcellulose sodium) (Xilong Chemical Co., Ltd.), Dextran sulfate sodium (DSS) from (MP Biomedical, LLC); chloral hydrate, and paraformaldehyde from (Shanghai Aladdin Biochemical Technology Co., Ltd.); KQ-250DB numerical control ultrasonic cleaner from (Gongyi Yuhua Instrument Co., Ltd.); Milli-Q ultrapure water machine from (Millipore company); 4°C refrigerator from (Haier company); –80°C ultra-low temperature refrigerator from (Thermo Fisher company); KDM type temperature control electric heating set from (juancheng Hualu electric heating instrument Co., Ltd.); SY-2000 rotary evaporator from (Shanghai Yarong biochemical instrument factory); SHB-III circulating water multi-purpose vacuum pump from (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.); and Doppler infrared imager from (Model: MOORLDI2-HIR, Moor Instruments Ltd Millwey Rise, AXMINSTER DEVON, EX13 5HU UK).
4
Isolation and Culture of H. pylori
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H. pylori Sydney strain SS1 (donated by the Third Military Medical University) was retrieved from a −80°C ultra-low temperature refrigerator (Thermo). The strain was then resuscitated at room temperature and mixed using a pipette gun. Subsequently, 100 µL of the bacterial solution was inoculated onto a solid culture medium. The bacterial solution was allowed to dry on the surface of the medium before being inverted and placed in a CO2 incubator for incubation at 37°C with 5% O2, 10% CO2, and 85% N2, creating a microaerobic environment. After 48–72 h, pinpoint-sized transparent colonies were observed. The presence of H. pylori was confirmed through HE staining, rapid urease testing, and catalase testing. A sterile inoculation loop was used to scrape a small quantity of cultured H. pylori colonies, which were evenly mixed with a PBS buffer. Subsequently, 100 µL of the mixture was aspirated and inoculated onto the next solid medium; the growth of colonies was observed within 48–72 h. Prior to administration in mice via gavage, H. pylori colonies were selected and dissolved in a PBS buffer. The concentration of the resulting liquid was controlled using turbidimetric methods to achieve a density of 1 × 109 colony forming units per milliliter of H. pylori mixture.
5
Colon Tissue Collection and Preservation
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After 5 wk, the mice were killed by cervical dislocation. The entirety of the blood was collected through the inferior vena cava, blood was centrifuged at 900 × g at 4°C for 10 min, and serum was removed and stored in a -80°C ultra-low-temperature refrigerator (Thermo Fisher Scientific Co. Ltd., Shanghai, China) for later use. After blood collection, the mice were dissected and colons removed. The length of each colon was measured, approximately 0.5 cm of colon tissue was cut and fixed in a 10% formalin solution, and the remaining colon tissue was preserved in a -80°C refrigerator for later use.
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