Captopril

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Captopril is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is a compound that can be used in various research applications, but a detailed description of its core function is not available.

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Lab products found in correlation

5 protocols using captopril

Peptide Synthesis and Digestion Assay

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The peptide AVFQHNCQE was synthesized by CASLO Aps. (Kongens Lyngby, Denmark) and its purity was 98.94%. Captopril was provided by Santa Cruz Biotechnology (Dallas, TX, United States). Pepsin, bile salts mixture, trypsin, α-chymotrypsin, porcine pancreatic lipase, colipase, trifluoroacetic acid (TFA), formic acid (FA) LC-MS grade, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (Madrid, Spain). Acetonitrile and methanol LC-MS grade were purchased from Merck (Darmstadt, Germany). The synthetic isotopically labeled peptide IFV*TGQDYNDK (V* = Val-13C5,15N) was obtained from Biomatik (Ontario, Canada) and used as internal standard (IS) for MS experiments. Chicken foot hydrolysate Hpp11 was obtained by our group following the procedure described by Bravo et al. [22 ,24 (link)]. All other chemical solvents used were of analytical grade.

Mas-Capdevila A., Iglesias-Carres L., Arola-Arnal A., Aragonès G., Muguerza B, & Bravo F.I. (2020). Implication of Opioid Receptors in the Antihypertensive Effect of a Novel Chicken Foot-Derived Peptide. Biomolecules, 10(7), 992.

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Enzymatic Hydrolysis and Antioxidant Evaluation

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Flavourzyme^® 1000 L (EC 3.4.11.1, 500 leucine amino peptidase units (LAPU)/g from Aspergillus oryzae) was kindly provided by Novozymes (Bagsværd, Denmark). ACE (EC 3.4.15.1), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), picrylsulfonic acid and acetonitrile for HPLC were purchased from Sigma-Aldrich (Madrid, Spain). Captopril (PubChem CID: 44093) and resveratrol were provided by Santa Cruz Biotechnology (Dallas, TX, USA) and Carl Roth (Karlsruhe, Germany), respectively. Folin–Ciocalteu reagent, coumaric acid, quercetin, gallic acid, catechin, and epicatechin were purchased from Fluka/Sigma-Aldrich. 4-Hydroxybenzoic acid, caffeic acid, procyanidin dimer B2, vanillic acid, and malvidin-3-O-glucoside were purchased from Extrasynthése (Lyon, France). Ferulic acid, cyanidin-3-O-rutinoside, and peonidin-3-O-rutinoside were purchased from PhytoLab (Vestenbergsgreuth, Germany). Analytical grade reagents were always used.

López-Fernández-Sobrino R., Margalef M., Torres-Fuentes C., Ávila-Román J., Aragonès G., Muguerza B, & Bravo F.I. (2021). Enzyme-Assisted Extraction to Obtain Phenolic-Enriched Wine Lees with Enhanced Bioactivity in Hypertensive Rats. Antioxidants, 10(4), 517.

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Transaortic Constriction and Pharmacological Interventions in Mice

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The authors declare that all supporting data are available within the article and its online supplementary files.
All experimental procedures were designed in accordance with the National Institutes of Health guidelines and approved by the Animal Welfare Committee and the Center for Laboratory Animal Medicine and Care in the University of Texas Health Science Center at Houston. Ten to eleven-week-old male C57BL/6J wild-type mice were purchased from Jackson Laboratory, Bar Harbor, ME, USA. At the age of 12 weeks, mice weighing 23-31grams (27.5±1.9g) were randomized to treatment with or without various drugs in the drinking water for three days, then underwent TAC or sham operation. Treatment was continued for two weeks post-operation. Losartan (sc-204796A, Santa Cruz Biotechnology, 0.6g/L)^{12 (link)}, captopril (sc-200566A, Santa Cruz Biotechnology, 75mg/L)¹¹, AT2R agonist C21 (a gift from Vicore Pharma, 300μg/kg/day),^{25 (link)} AT2R antagonist PD123319 (P186-10MG, Sigma-Aldrich, 3mg/kg/day)^{20 (link)} and MasR agonist AVE0991 (a gift from Sanofi-Aventis, Frankfurt/Main, 576μg/kg/day)^{26 (link)} were administrated 1 hour prior operation to two weeks post-operation via intraperitoneal injection. An illustration of the signaling pathways and the drug targets are presented in Supplemental figure 1A.

Zhou Z., Peters A.M., Wang S., Janda A., Chen J., Zhou P., Arthur E., Kwartler C.S, & Milewicz D.M. (2019). Reversal of Aortic Enlargement Due to Increased Biomechanical Forces Requires AT1R Inhibition in Conjunction with AT2R Activation. Arteriosclerosis, thrombosis, and vascular biology, 39(3), 459-466.

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Chicken Feet Protease Extraction and ACE Inhibition

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Chicken feet from Gallus gallus domesticus were provided by a local farm (Granja Gaià, La Riera de Gaià, Spain). Protamex^® (Novozymes, Bagsværd, Denmark) (EC 3.4.21.62 and 3.4.24.28, 1.5 AU/g from Bacillus licheniformis and Bacillus amyloliquefaciens), was kindly provided by Novozymes (Bagsværd, Denmark). ACE (angiotensin-converting enzyme, EC 3.4.15.1), N-Hippuryl-His-Leu (Hip-His-Leu), Captopril (PubChem CID: 44093) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA) and o-aminobenzoylglicil-p-nitrofenilalanilprolina (o-Abz-Gly-p-Phe(NO₂)-Pro-OH, PubChem CID: 128860) was provided by Bachem Feinchemikalien (Bubendorf, Switzerland). Acetylcholine (PubChem CID: 187), methoxamine hydrochloride (PubChem CID: 6081) and heparin heparin (PubChem CID: 772) were purchased from Sigma-Aldrich (Madrid, Spain). All other chemical solvents used were of analytical grade.

Mas-Capdevila A., Pons Z., Aleixandre A., Bravo F.I, & Muguerza B. (2018). Dose-Related Antihypertensive Properties and the Corresponding Mechanisms of a Chicken Foot Hydrolysate in Hypertensive Rats. Nutrients, 10(9), 1295.

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Functionalized PEG-Ang Peptide Conjugation

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Carboxylic‐acid and Boc‐amine‐ or methoxy‐functionalized PEG (2000 and 5000 Da) were purchased from JenKem Technology USA Inc. (Allen, TX, USA). Lysine N‐modified Ang‐I and Ang‐II (Lys‐Ang‐I and Lys‐Ang‐II) (purity >98%) were purchased from Genscript (Piscataway, NJ, USA). EXP3174 and captopril were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Fura‐2AM was purchased from Life Technologies (Carlsbad, CA, USA). DPBS, Lipofectamine 2000, TAMRA‐amine, Pierce BCA Assay kit, CellMask Deep Red Plasma Membrane Stain (CMDR), CellTracker Deep Red (CTDR), and Green (CTG) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Hoechst 33258 was obtained from Polysciences Inc. (Warrington, PA, USA). Integrin‐α8 goat antibody was purchased from R&D Systems (Minneapolis, MN, USA). Cy2‐anti‐goat secondary antibody was obtained from Jackson Immuno Research Labs (PA, USA). CXN2‐HA‐AT1R‐YFP was a gift from Yusuke Ohba (Addgene plasmid #101659; http://n2t.net/addgene:101659; RRID: Addgene_101659). All other materials and reagents in analytical grade were obtained from Sigma‐Aldrich (Taufkirchen, Germany).

Maslanka Figueroa S., Fleischmann D., Beck S., Tauber P., Witzgall R., Schweda F, & Goepferich A. (2020). Nanoparticles Mimicking Viral Cell Recognition Strategies Are Superior Transporters into Mesangial Cells. Advanced Science, 7(11), 1903204.

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