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Ab269542

Manufactured by Abcam
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Sourced in United Kingdom

Ab269542 is a laboratory product from Abcam. It is a protein-based reagent, but further details about its core function are not available in an unbiased and factual manner without extrapolation.

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Lab products found in correlation

Corresponding assay kit, by Nanjing Jiancheng (1 mentions) Ab197742, by Abcam (1 mentions) Fgf19, by R&D Systems (1 mentions) Glp 1, by Abcam (1 mentions) Il 1β, by Abcam (1 mentions) 7180 clinical analyzer, by Hitachi (1 mentions) Insulin, by Crystal Chem (1 mentions) C peptide, by Crystal Chem (1 mentions)

7 protocols using ab269542

1

Measuring Inflammatory Cytokines and LBP

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The LPS binding protein (LBP) concentration was measured using an ELISA kit (ab269542, Abcam, Cambridge, UK), and a mouse Cytokine 23-Plex Assay (Kit Bio-Rad, Hercules, CA, USA) was used to measure the concentration of serum inflammatory factors. All procedures were performed according to the kit manuals.
Shen J., Wang S., Huang Y., Wu Z., Han S., Xia H., Chen H, & Li L. (2023). Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice. Nutrients, 15(19), 4256.
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2

Quantifying Mouse Serum LBP Levels

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A mouse LBP enzyme-linked immunosorbent assay (ELISA) kit (LPS binding protein) (Abcam, ab269542) was used for quantification of serum LBP concentration according to the manufacturer’s protocol.
Wang K., Wu W., Jiang X., Xia J., Lv L., Li S., Zhuge A., Wu Z., Wang Q., Wang S, & Li L. (2022). Multi-Omics Analysis Reveals the Protection of Gasdermin D in Concanavalin A-Induced Autoimmune Hepatitis. Microbiology Spectrum, 10(5), e01717-22.
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3

Cytokine Profiling in Murine Bone Marrow Transplant

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Serum samples were collected from whole blood without anticoagulants and centrifuged at 4℃/1000 g for 10 min to collect the serum supernatant for analysis. Then, the level of murine TNF‐α, IL‐1β, IFN‐γ, MCP‐1, IL‐6, IL‐18 (CUSABIO) and LBP (Abcam, ab269542) were assayed quantitatively via ELISA kits (CUSABIO) between day 4 and 6 after bone marrow transplantation (BMT), following the manufacture protocols. Next, we analysed microwell strips by using a microwell reader (Molecular Devices), weighed the extracted colon tissues, homogenized tissues in the tissue extraction reagent for 3 min on ice and acquired the supernatants after 4℃/10,000 g centrifugation for 5 min, then, stored at −20℃. Finally, we detected the levels of IL‐1β, TNF‐α and IL‐6 in colon homogenates through ELISA assay.
Zhao Y., Huang J., Li T., Zhang S., Wen C, & Wang L. (2022). Berberine ameliorates aGVHD by gut microbiota remodelling, TLR4 signalling suppression and colonic barrier repairment for NLRP3 inflammasome inhibition. Journal of Cellular and Molecular Medicine, 26(4), 1060-1070.
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4

Hepatic Metabolic Biomarkers Evaluation

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The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using Catalyst One Chemistry Analyzer. Total triglyceride (TG), total cholesterol (TC), free fatty acids (FFAs), superoxide dismutase (SOD), and malondialdehyde (MDA) in extracted hepatic samples were measured with corresponding assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China). Serum insulin and LPS-binding protein (LBP) were determined using ELISA kits (Crystalchem, 90080; Abcam, ab269542, respectively) according to the manufacturer's instructions.
Wang R.R., Zhang L.F., Chen L.P., Wang J.Y., Zhang L., Xu Y.S., Yang P.L., Ji G, & Liu B.C. (2021). Structural and Functional Modulation of Gut Microbiota by Jiangzhi Granules during the Amelioration of Nonalcoholic Fatty Liver Disease. Oxidative Medicine and Cellular Longevity, 2021, 2234695.
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5

Quantifying Mouse Serum LBP Levels

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A mouse LBP enzyme-linked immunosorbent assay (ELISA) kit (LPS binding protein) (Abcam, ab269542) was used for quantification of serum LBP concentration according to the manufacturer’s protocol.
Wang K., Wu W., Jiang X., Xia J., Lv L., Li S., Zhuge A., Wu Z., Wang Q., Wang S, & Li L. (2022). Multi-Omics Analysis Reveals the Protection of Gasdermin D in Concanavalin A-Induced Autoimmune Hepatitis. Microbiology Spectrum, 10(5), e01717-22.
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6

Measuring Inflammatory Cytokines and LBP

Check if the same lab product or an alternative is used in the 5 most similar protocols
The LPS binding protein (LBP) concentration was measured using an ELISA kit (ab269542, Abcam, Cambridge, UK), and a mouse Cytokine 23-Plex Assay (Kit Bio-Rad, Hercules, CA, USA) was used to measure the concentration of serum inflammatory factors. All procedures were performed according to the kit manuals.
Shen J., Wang S., Huang Y., Wu Z., Han S., Xia H., Chen H, & Li L. (2023). Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice. Nutrients, 15(19), 4256.
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7

Metabolic Biomarker Profiling in Diabetes

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In the cohort study, participants’ blood HbA1c, FBG, C-peptide, WBC, NEUT, LYMPH, TC, TG, HDL-C, and LDL-C levels were measured according to uniform national quality control protocols. Serum GLP-1 (Abcam, Cambridge, UK; ab184857), FGF19 (R&D Systems, Minneapolis, MN, USA; DF1900), LBP (Panchao Biotechnology, Shanghai, China; PCDBH0284), and IL-1β (Panchao Biotechnology, PCDBH0247) concentrations were measured using enzyme-linked immunosorbent assay kits, according to the manufacturer’s instructions.
In the animal experiments, HbA1c was determined by an enzymatic method using a Hitachi 7180 Clinical Analyzer (Hitachi, Tokyo, Japan). Serum insulin (Crystal Chem Inc., 90080), C-peptide (Crystal Chem Inc., 90050), IL-1β (Abcam, ab197742), and LBP (Abcam, ab269542) concentrations were measured using the mouse ELISA kits as per the manufacturer’s instructions.
Yuan X., Wang R., Han B., Sun C., Chen R., Wei H., Chen L., Du H., Li G., Yang Y., Chen X., Cui L., Xu Z., Fu J., Wu J., Gu W., Chen Z., Fang X., Yang H., Su Z., Wu J., Li Q., Zhang M., Zhou Y., Zhang L., Ji G, & Luo F. (2022). Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes. Nature Communications, 13, 6356.
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