Anti rab40c

The following antibodies were used in this study: anti-FLAG (clone M2, WB 1:1,000; Sigma-Aldrich), anti-GAPDH (WB 1:5,000; UBPBio), FAK S910 (WB 1:1,000, 44-596G; Invitrogen), total FAK (WB 1:1,000, 610087; BD Biosciences), and paxillin (IF 1:500; Transduction Labs). Anti-HA (WB 1:500, IP 2 μg/1 mg cell lysate, SC F-7), anti–α-tubulin (WB 1:5,000, 23948), anti-Rab40c (WB 1:500, H-8 sc514826), cul-5 (WB 1:500, H-300), and mouse ANKRD28 (WB 1:500) were purchased from Santa Cruz Biotechnology. MOB1(E1N9D) and p-MOB1(D2F10) were purchased from Cell Signaling Technology. Rabbit anti-SAPS1/2/3, ANKRD28/52, and PP6c were purchased from Bethyl Laboratories. Specificity of anti-Rab40c antibody was confirmed by immunoblotting lysates derived from cells expressing FLAG-Rab40a, FLAG-Rab40b, or FLAG-Rab40c constructs (Fig S2A).

The following antibodies were used in this study: anti-FLAG (WB 1:1,000, F3165; Sigma), anti-EPLIN (WB 1:1,000, IF 1:100, 50311; Cell Signaling Technology), anti-EPLIN (WB 1:1,000, IF 1:100, sc-136399; Santa Cruz Biotechnology), anti-EPLIN (WB 1:1,000, immunoprecipitation [IP] 1 μg/1 mg lysate, immunohistochemistry [IHC] 1:200, 16639–1-AP; Proteintech), anti-HA (WB 1:500, IP 2 µg/1 mg cell lysate, SC F-7; Santa Cruz Biotechnology), Alexa Fluor 568–phalloidin (IF 1:1,000, A1238; Life Technologies), SiR-actin (1:10,000, CY-SC001; Cytoskeleton), anti–β-tubulin (WB 1:2,500, 926–42211; LI-COR), anti–α-tubulin (WB 1:2,500, 23948; Santa Cruz Biotechnology), pFAK Y397 (WB 1:1,000, ab8129; Abcam), FAK S910 (WB 1:1,000, 44-596G; Invitrogen), anti–E-Cadherin (WB 1:1,000, IHC 1:200, 3195; Cell Signaling Technology), total FAK (WB 1:1,000, 610087; BD Biosciences), anti-zyxin (IF 1:1,000, ab50391; Abcam), anti-Rab40b (WB 1:500, LS-C353287; LSBio), anti-Rab40c (WB 1:500, H-8 sc-514826; Santa Cruz Biotechnology), and anti-CD63 (IF 1:100; gift from Andrew Peden, University of Shefield, Shefield, UK).