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Mir 149 5p inhibitor

Manufactured by GenePharma
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Sourced in China

The MiR-149-5p inhibitor is a lab equipment product designed to inhibit the expression of the miR-149-5p microRNA. It is a synthetic oligonucleotide that binds to and blocks the function of the miR-149-5p, a small non-coding RNA molecule involved in various cellular processes.

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Lab products found in correlation

Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Mir 149 5p mimic, by GenePharma (2 mentions) Mirna nc, by GenePharma (1 mentions) Pcdna3.1 empty vector, by Thermo Fisher Scientific (1 mentions) Si nc, by GenePharma (1 mentions) Mir nc, by GenePharma (1 mentions) Pcdna3.1 nc pcdna nc, by GenePharma (1 mentions) Si blacat1 1, by GenePharma (1 mentions) Si blacat1 2, by GenePharma (1 mentions) Inhibitor control, by GenePharma (1 mentions) C5orf66 as1 sirna, by Thermo Fisher Scientific (1 mentions) Control sirna, by Thermo Fisher Scientific (1 mentions)

4 protocols using mir 149 5p inhibitor

1

YWHAZ Overexpression Vector Construction

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For the YWHAZ overexpression vector, the full sequence of YWHAZ was introduced into pcDNA3.1 empty vector (pc, Invitrogen, Waltham, MA, USA), named as pc-YWHAZ, and pcDNA3.1 empty vector acted as a negative control (pc-NC). CircNHSL1 small interfering RNA (si-circNHSL1) and its negative control (si-NC), miR-149-5p mimic, miR-149-5p inhibitor and their negative controls (miRNA NC and inhibitor NC) were obtained from GenePharma (Shanghai, China). According to the manufacturer’s instructions, Lipofectamine 3000 reagent (Invitrogen) was applied for transfection.
Hui C., Tian L, & He X. (2020). Circular RNA circNHSL1 Contributes to Gastric Cancer Progression Through the miR-149-5p/YWHAZ Axis. Cancer Management and Research, 12, 7117-7130.
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2

Investigating miR-149-5p Regulation

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B3GNT3 siRNA or shRNA vectors and relative negative controls, miR-149-5p mimics or miR-149-5p inhibitor was purchased from GenePharma (Shanghai, China). Lipofectamine 3000 (Thermo fisher Scientific, CA USA) was utilized for cell transfection. The sequences of synthetic oligonucleotides about miR-149-5p mimics, inhibitor and relative negative controls in Supplementary Table 2.
Sun Y., Liu T., Xian L., Liu W., Liu J, & Zhou H. (2020). B3GNT3, a Direct Target of miR-149-5p, Promotes Lung Cancer Development and Indicates Poor Prognosis of Lung Cancer. Cancer Management and Research, 12, 2381-2391.
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3

siRNA and miRNA Regulation of KIF2A

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The siRNA-negative control (si-NC), si-BLACAT1-1, si-BLACAT1-2, miR-NC, miR-149-5p mimics, miR-149-5p inhibitor, pcDNA3.1-NC (pcDNA-NC) and pcDNA3.1-KIF2A (pcDNA-KIF2A) were synthesized by Shanghai Genepharma (Shanghai, China). AGS and MKN45 cells grown to 80% confluence were transfected or co-transfected with the above agents using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s protocol.
Wang Z., Liu X., Liu X, & Niu D. (2020). Long Non-Coding RNA BLACAT1 Promotes the Tumorigenesis of Gastric Cancer by Sponging microRNA-149-5p and Targeting KIF2A. Cancer Management and Research, 12, 6629-6640.
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4

Silencing C5orf66-AS1 and Inhibiting miR-149-5p in U2OS Cells

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The control-small interfering (si)RNA, C5orf66-AS1-siRNA, inhibitor control (5′-CAGUACUUUUGUGUAGUACAA-3′) and miR-149-5p inhibitor (5′-GGGAGUGAAGACACGGAGCCAGA-3′) were synthesized by Shanghai GenePharma Co., Ltd. U2OS cells were transfected with 1 µM control-siRNA, 1 µM C5orf66-AS1-siRNA, 100 nM inhibitor control, 100 nM miR-149-5p inhibitor, 1 µM C5orf66-AS1-siRNA + 100 nM inhibitor control, or 1 µM C5orf66-AS1-siRNA + 100 nM miR-149-5p inhibitor at 37°C for 48 h using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. At 48 h following transfection, transfection efficiency was determined using RT-qPCR analysis.
Zhang H, & Song J. (2021). Knockdown of lncRNA C5orf66-AS1 inhibits osteosarcoma cell proliferation and invasion via miR-149-5p upregulation. Oncology Letters, 22(5), 757.
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