Plv egfp cre

Manufactured by Addgene

The PLV-EGFP-Cre is a plasmid that expresses the Cre recombinase enzyme fused to enhanced green fluorescent protein (EGFP). The Cre recombinase is a site-specific DNA recombinase derived from bacteriophage P1 that catalyzes the excision of DNA sequences flanked by loxP sites.

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Lab products found in correlation

Pmdg 2, by Addgene (1 mentions) Pspax2, by Addgene (1 mentions)

2 protocols using plv egfp cre

Cre Recombinase Excision Verification

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Cells were transfected with pLV-EGFP-Cre (#86805; Addgene). 24 h later, cells were FACS-sorted based on the EGFP signal. Successful excision of antibiotic resistance genes was verified by PCR on gDNA and by culturing cells in selective medium.

Droubi A., Wallis C., Anderson K.E., Rahman S., de Sa A., Rahman T., Stephens L.R., Hawkins P.T, & Lowe M. (2022). The inositol 5-phosphatase INPP5B regulates B cell receptor clustering and signaling. The Journal of Cell Biology, 221(9), e202112018.

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Lentiviral Vector Production Protocol

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pLenti-Largen T antigen (Cat#18922), Lenti-pHIV-eGFP (Cat#21373), PSPAX2 (Cat#12260), PMD.G2 (Cat#12259), pLV-eGFP-Cre (Cat#86805) were purchased from Addgene. Total 13ug pasmids (object gene, PSPAX2, PMD.G2 ) was co-transfected into 75cm² flask with 293T cells in 10 ml DMEM Medium, 6-7ml fresh DMEM medium were changed after plasmids co-transfected 8-10 h. The medium was collected and centrifuged at 4°C in 500x g for 10 min after transfected 72 h. The medium with virus was aliquoted and stored at −80°C.

Yokota T., McCourt J., Ma F., Ren S., Li S., Kim T.H., Kurmangaliyev Y.Z., Nasiri R., Ahadian S., Nguyen T., Haw M., Zhou Y., Wu R., Rodriguez A., Cohn W., Wang Y., Whitelegge J., Ryazantsev S., Khademhosseini A., Teitell M.A., Chiou E., Birk D.E., Rowat A.C., Crosbie R.H., Pellegrini M., Seldin M., Lusis A.J, & Deb A. (2020). Type V collagen in scar tissue regulates the size of scar after heart injury. Cell, 182(3), 545-562.e23.

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