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The HT1080 6TG is a cell line derived from a human fibrosarcoma. It is commonly used in research as a model system for various cellular and molecular studies.

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Lab products found in correlation

Ku55933, by Bio-Techne (2 mentions) Dmem glutamax, by Thermo Fisher Scientific (2 mentions) Dmem glutamax, by American Type Culture Collection (2 mentions) Parpi, by Selleck Chemicals (2 mentions) Dna pkcsi, by Bio-Techne (2 mentions) Rad51i ri 1, by Selleck Chemicals (2 mentions) Olaparib, by Selleck Chemicals (2 mentions)

2 protocols using ht1080 6tg

1

Establishing Cell Cultures and Inhibitor Treatments

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HT1080, HT1080 6TG (ATCC) and their derivatives cells were grown in Glutamax-DMEM supplemented with 0.1 mM nonessential amino acids and 10% bovine growth serum. Human IMR-90 primary lung fibroblasts transformed with E6E7 1 (link) were grown in Glutamax-DMEM (Gibco) supplemented with 0.1 mM nonessential amino acids and 15% fetal bovine serum. All cells were grown at 7.5% CO2 and 3% O2. All cell lines were purchased from ATCC or have been commercially authenticated and tested free of mycoplasma. When indicated, cells were treated with the following inhibitors: ATMi (KU-55933, Tocris, 10µM), DNA-PKcsi (NU-7441, Tocris, 1µM), PARPi (Olaparib, Selleck Chemical, 10µM), RAD51i (RI-1, Selleck Chemical, 20µM).
Arnoult N., Correia A., Ma J., Merlo A., Garcia-Gomez S., Maric M., Tognetti M., Benner C.W., Boulton S.J., Saghatelian A, & Karlseder J. (2017). Regulation of DNA Repair pathway choice in S/G2 by the NHEJ inhibitor CYREN. Nature, 549(7673), 548-552.
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2

Establishing Cell Cultures and Inhibitor Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols
HT1080, HT1080 6TG (ATCC) and their derivatives cells were grown in Glutamax-DMEM supplemented with 0.1 mM nonessential amino acids and 10% bovine growth serum. Human IMR-90 primary lung fibroblasts transformed with E6E7 1 (link) were grown in Glutamax-DMEM (Gibco) supplemented with 0.1 mM nonessential amino acids and 15% fetal bovine serum. All cells were grown at 7.5% CO2 and 3% O2. All cell lines were purchased from ATCC or have been commercially authenticated and tested free of mycoplasma. When indicated, cells were treated with the following inhibitors: ATMi (KU-55933, Tocris, 10µM), DNA-PKcsi (NU-7441, Tocris, 1µM), PARPi (Olaparib, Selleck Chemical, 10µM), RAD51i (RI-1, Selleck Chemical, 20µM).
Arnoult N., Correia A., Ma J., Merlo A., Garcia-Gomez S., Maric M., Tognetti M., Benner C.W., Boulton S.J., Saghatelian A, & Karlseder J. (2017). Regulation of DNA Repair pathway choice in S/G2 by the NHEJ inhibitor CYREN. Nature, 549(7673), 548-552.
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