Lgr5 dtr egfp mice

Mist1-CreERT2 mice [28 (link)], Wnt5a^flox/flox mice [29 (link)], and Wnt5a+/− [30 (link)] mice have been described previously and were purchased from Jackson lab. Lgr5-DTR-eGFP mice were provided by Genentech. Cxcr4-eGFP mice were kindly provided by Richard J. Miller (Northwestern University Medical School, USA). Cag-CreERT mice, R26-Tdtomato mice and C57/B6 wild-type (WT) mice were purchased from Jackson Lab. All animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) at Columbia University.
A detailed description of all methods used in this study can be found in the supplementary information.

All animals used in this study received humane care in compliance with the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH Publication, 1996 edition, and the protocol was approved by the Institutional Animal Care Committee of Universityof Washington. The C57BL/6 and SCID/Beige mice were purchased from Charles River (Wilmington, MA). Lgr5-DTR-eGFP mice were originally generated by Genentech (Tian et al., 2011 (link)) and were kindly provided by Dr. Noah Shroyer at the Baylor College of Medicine. Krt7-CreER^T2 was kindly provided by Dr. Jianwen Que at Columbia University. Krt8-CreER^T2 was made by our laboratory as described (Zhang et al., 2012 ). C57BL/6-Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J, B6.129P2-Lgr5^{tm1(cre/ERT2)Cle}/J, Col1a2-CreER^T2, Pdgfrβ-CreER^T2, NG2-CreER^T2, B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/J mice were from the Jackson Laboratory (Bar Harbor, ME). Male mice at the age of E15.5 to postnatal 27 weeks were used. Mice were genotyped by polymerase chain reaction using mouse genomic DNA from tail biopsy specimens. The sequences of genotyping primers and the expected band sizes for PCR are listed in Table S3. PCR products were separated electrophoretically on 1% agarose gels and visualized via ethidium bromide under UV light.