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Rs 12m

Manufactured by Bruker
Sourced in United States

The RS-12M is a laboratory instrument designed for the analysis of solid and liquid samples. It provides precise measurements of various physical and chemical properties of the samples. The core function of the RS-12M is to perform accurate and reliable sample analysis, without making any interpretations or extrapolations about its intended use.

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2 protocols using rs 12m

1

Characterizing Probe Tips for Nanomechanical Mapping

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Before a relocation system with a built-in characterizer could be prepared, the potential characterizers had to be characterized themselves. A detailed description is given in ESI (SI-1, step 1). Briefly, a sharp nitride lever tip (k = 0.12 N m−1, SNL-10, Bruker, USA) was characterized by scanning a titanium roughness sample (RS-12M, Bruker, USA, ESI-Fig. 1a) in Peak Force Quantitative Nanomechanical Mapping (PFQNM) mode in air with an atomic force microscope (AFM, Dimension Icon, Bruker Corporation, USA) and further used as built-in characterizer for the kaolinite modified probe. The titanium roughness sample allows the characterization of the very end of the AFM tip25 (link) which was needed to precisely define the dilation length of the ∼40 nm thick kaolinite sheets. As a characterizer for the bacterial modified tipless probe, the more elongated Tap150A probe (k = 5 N m−1, Bruker, USA) was characterized by tapping mode using a test grating TGT1 (NT-MDT Spectrum Instruments, USA). The TGT1 characterizes the overall tip shape at a sub-micron scale, which was essential for the morphology imaging of the bacteria in μm scale. We always used the frame down command, i.e., a horizontal fast scan direction. The resultant images were flattened by first order and subjected to blind tip reconstruction analysis using NanoScope Analysis software (version 8.15, Bruker).
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2

Calcite Surface Preparation and Characterization

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The calcite material analyzed was optical-quality
Iceland spar (purchased from Geocity AB, Stockholm, mined in Madagascar)
cleaved to provide a uniform fresh surface with stainless steel chisel
and hammer,23 (link) following the natural crystal
angles of the rhombohedron along the dominant {101̅4} cleavage
plane. This highly hygroscopic and wettable surface32 (link),33 (link) was immediately purged with pressurized nitrogen (industrial quality:
nitrogen ≥99.9 vol %, oxygen ≤20 ppm, water ≤10
ppm). At least three samples for each study were analyzed, each being
a few millimeters thick with an approximate surface area of 50–100
mm2, and with no evidence of microcracks or excessive surface
steps. Epoxy glue (Bostik, France) was used for sample attachment
to the magnetic disk used in the AFM studies. A sapphire calibration
sample (Bruker) was used for deflection sensitivity calibration of
the AFM probes, and a titanium roughness sample (RS-12M, Bruker) for
the determination of the probes’ tip end radii. Stearic acid
(C18) (Sigma-Aldrich, ≥97.0%) was used for modification
of the calcite surface.
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