Anti transferrin receptor clone h68

For IF, cells were plated onto collagen IV-coated glass coverslips (10µg/ml) and then fixed with 4% paraformaldehyde (PFA) in PBS (phosphate-buffered saline) for 15min, permeabilized with 0.2% Triton X-100 in PBS for 5 min, blocked with 5% goat serum in PBS for 1h, and incubated with Rab13 rabbit polyclonal antibody (Novus Biologicals, cat# NBP1-85799, 1/200 dilution) for 1h. Secondary antibodies were conjugated with Alexa 647 (Thermo Fisher Scientific).
For Western blot detection the following antibodies were used: anti-Rab13 rabbit polyclonal (Novus Biologicals, cat# NBP1-85799, 1/1,000 dilution), anti-GFP rabbit polyclonal (Invitrogen, cat# A11122, 1/2,000 dilution), anti-RhoGDI, anti-Transferrin receptor (clone H68.4, ThermoFisher Scientific, cat# 13-6800), anti-a-tubulin mouse monoclonal (Sigma-Aldrich, cat# T6199, 1/10,000 dilution), and anti-GAPDH rabbit monoclonal 14C10 (Cell Signaling Technology, cat# 2118, 1/2,000 dilution), anti-phospho-FAK(Y397) (Cell Signaling Technology, cat# 3283), anti-REP-1 (Abcam, cat# 134964), anti-RabGDI (Santa Cruz Biotechnology, cat# sc-374649), anti-RABIF mouse monoclonal antibody D-12 (Santa Cruz biotechnology, cat# sc-390759, 1/1,000 dilution).