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Rabbit monoclonal anti activated caspase 3

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Rabbit monoclonal anti-activated caspase-3 is an antibody that specifically binds to the cleaved, active form of caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway.

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Lab products found in correlation

Rabbit anti actin, by Merck Group (5 mentions) Rabbit monoclonal anti cytochrome c, by Cell Signaling Technology (4 mentions) Rabbit monoclonal anti parp, by Cell Signaling Technology (4 mentions) Rabbit monoclonal anti psmad1 5, by Cell Signaling Technology (4 mentions) Rabbit polyclonal survivin, by Novus Biologicals (3 mentions) Rabbit monoclonal anti c iap1, by Cell Signaling Technology (3 mentions) Rabbit monoclonal anti aif, by Cell Signaling Technology (3 mentions) Anti gapdh, by Merck Group (3 mentions) Mouse monoclonal anti spectrin, by Merck Group (2 mentions) Rabbit monoclonal anti smac diablo, by Cell Signaling Technology (2 mentions) Polyclonal rabbit anti gapdh, by Merck Group (2 mentions) Rabbit monoclonal anti ptak1, by Cell Signaling Technology (2 mentions) Rabbit monoclonal anti smac, by Cell Signaling Technology (2 mentions) Anti spectrin, by Merck Group (2 mentions) Gapdh, by Merck Group (2 mentions) Enhanced chemiluminescence system, by Cytiva (1 mentions) Rabbit monoclonal xiap, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Rabbit anti-activated caspase 3 Rabbit monoclonal anti-caspase-3 Anti-activated caspase-3 Activated caspase-3 Monoclonal anti-caspase-3 Rabbit anti-caspase-3 Anti-caspase-3 Caspase-3 Monoclonal rabbit Caspases

5 protocols using rabbit monoclonal anti activated caspase 3

1

Western Blot Analysis of Apoptosis Markers

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Western blot analysis was performed as previously reported36 (link). In brief, total cellular protein concentration was determined using BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were , rabbit monoclonal anti-Smac/Diablo, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit anti-actin, an affinity isolated antigen specific antibody (Sigma, Saint Louis, MO), and rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA). Rabbit monoclonal anti-pAMPKα(T172), rabbit monoclonal anti-AMPK, rabbit monoclonal-pACC(Ser79), rabbit monoclonal-anti-BMPR2 and rabbit monoclonal COX1/MT C01 were purchased from Cell signaling Technology, USA.
Mondal A., Jia D., Bhatt V., Akel M., Roberge J., Guo J.Y, & Langenfeld J. (2022). Ym155 localizes to the mitochondria leading to mitochondria dysfunction and activation of AMPK that inhibits BMP signaling in lung cancer cells. Scientific Reports, 12, 13135.
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2

Protein Extraction and Western Blot Analysis

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Total cellular protein was prepared using RIPA buffer containing a protease inhibitor cocktail and protein concentration was measured using the BCA assay as described [4 (link)]. In brief, protein was analyzed by SDS-PAGE, transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). After blocking, the blots were incubated overnight at 4 °C with the appropriate primary antibody in Tris-buffered saline with 1 % Tween (TBST) and 5 % BSA. Secondary antibodies were applied for 1 h at room temperature. Specific proteins were detected using the enhanced chemiluminescence system (Amersham, Arlington Heights, IL). The primary antibodies that were used were rabbit monoclonal anti-pSmad 1/5, rabbit monoclonal anti-pSmad2, rabbit monoclonal anti-Smad2, rabbit monoclonal anti-pTAK1, rabbit monoclonal XIAP, rabbit anti-monoclonal p-p65, rabbit monoclonal anti-activated caspase-3, rabbit polyclonal anti-BMPRII, and rabbit monoclonal anti-EGR-1 (Cell signaling Technology, Danvers MA), Rabbit monoclonal anti-TAK1 (Invitrogen, Grand Island NY), rabbit anti-actin, an affinity isolated antigen specific antibody (Sigma, Saint Louis, MO), rabbit monoclonal anti-Id1 and rabbit monoclonal anti-Id3 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-GAPDH (Sigma, St. Louis, MO) and rabbit polyclonal pMEK-1/2 (Cell Signaling, Danvers MA).
Augeri D.J., Langenfeld E., Castle M., Gilleran J.A, & Langenfeld J. (2016). Inhibition of BMP and of TGFβ receptors downregulates expression of XIAP and TAK1 leading to lung cancer cell death. Molecular Cancer, 15, 27.
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3

Comprehensive Western Blot Analysis of Apoptosis Markers

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Western blot analysis was performed as previously reported [3] . In brief, total cellular protein concentration was determined using the BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/DIABLO, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit anti-actin, an a nity isolated antigen speci c antibody (Sigma, Saint Louis, MO), rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).
Blots suggesting regulation of loading controls actin or GAPDH by Ym155 and/or JL5 were then probed with the spectrin, which is also a cytoskeletal protein.
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce Apoptosis-Inducing Factor (AIF) caspase independent cell death.
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4

Comprehensive Western Blot Analysis of Apoptosis Markers

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Western blot analysis was performed as previously reported [3] . In brief, total cellular protein concentration was determined using the BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/DIABLO, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit anti-actin, an a nity isolated antigen speci c antibody (Sigma, Saint Louis, MO), rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).
Blots suggesting regulation of loading controls actin or GAPDH by Ym155 and/or JL5 were then probed with the spectrin, which is also a cytoskeletal protein.
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce Apoptosis-Inducing Factor (AIF) caspase independent cell death.
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5

Western Blot Analysis of Cell Signaling

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Western blot analysis was performed as previously reported [3] . In brief, total cellular protein concentration was determined using BCA method then separated by SDS-PAGE and transferred to nitrocellulose (Schleicher and Schuell, Keene, NH). The primary antibodies used were rabbit monoclonal anti-XIAP, rabbit monoclonal anti-Smac/Diablo, rabbit monoclonal anti-cytochrome c, rabbit monoclonal anti-c-IAP1, rabbit monoclonal anti-pTAK1, rabbit monoclonal anti-activated caspase-3, rabbit monoclonal anti-PARP, rabbit monoclonal anti-AIF, rabbit monoclonal anti-pSmad1/5, (Cell signaling Technology, MA, USA), rabbit monoclonal anti-Id1 (Calbioreagents, San Mateo, CA), rabbit polyclonal anti-Smad 1/5 (Upstate Biotechnology, NY, USA), rabbit polyclonal survivin (Novus Biologicals, CO, USA), rabbit antiactin, an a nity isolated antigen speci c antibody (Sigma, Saint Louis, MO), and rabbit polyclonal anti-GAPDH (Sigma) and mouse monoclonal anti-Spectrin (EMD Millipore, CA, USA).
Mondal A., NeMoyer R., Langenfeld E., Glover D., Scott M.J., Lairson L., Zloza A., Augeri D.J., Gilleran J., Peng Y., Roberge J.Y., & Langenfeld J. (2020). Bone morphogenetic protein (BMP) receptor inhibitor JL5 synergizes with Ym155 to induce AIF-caspase independent cell death.
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