Panc 1

We purchased the human pancreatic cancer cell lines (PK-1, PK-8, KLM-1, Panc-1 and MIAPaca2) from RIKEN BioResouce Research Center (RIKEN BRC, Ibaraki, Japan) and another human pancreatic cancer cell line (BxPC-3) and Plat-A amphotropic retrovirus packaging cells from American Type Culture Collection (ATCC, Manassas, VA, USA). We maintained PK-1, PK-8, KLM-1, Panc-1 and BxPC-3 in RPMI-1640 (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin (Life Technologies, Carlsbad, CA, USA) and 100 μg/ml streptomycin (Life Technologies) at 37°C in a humidified 5% CO₂ incubator. We maintained MIAPaca2 and Plat-A in DMEM (Nacalai Tesque) supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. In Plat-A culture, 1 μg/ml of puromycin (Nacalai Tesque) and 10 μg/ml of blasticidin (Funakoshi, Tokyo, Japan) were added.

We purchased human PDAC cell lines (PK-1, PK-8, KLM-1, Panc-1 and MIAPaca2) from RIKEN BioResource Research Center (RIKEN BRC, Ibaraki, Japan) and another human PDAC cell line (BxPC-3) from American Type Culture Collection (ATCC, Manassas, VA, USA). We maintained PK-1, PK-8, KLM-1, Panc-1 and BxPC-3 cells in RPMI-1640 (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (FBS) (Merck KGaA, Darmstadt, Germany, or Life Technologies, Carlsbad, CA, USA), 100 U/ml penicillin (Life Technologies) and 100 µg/ml streptomycin (Life Technologies). We maintained MIAPaca2 cells in DMEM (Nacalai Tesque) supplemented with 10% FBS, 50 U/ml penicillin and 50 µg/ml streptomycin. We maintained all cell lines at 37 °C in a humidified atmosphere containing 5% CO₂.

The human OSCC cell line (HSC-3) and the human gastric cancer cell lines (MKN45 and NUGC-4) were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). The human pancreatic cancer cell line (PANC-1) was obtained from the Cell Resource Center for Biomedical Research Institute of Development, Aging, and Cancer at Tohoku University (Sendai, Japan). Chinese hamster ovary (CHO)-K1 and P3X63Ag8U.1 (P3U1; a mouse multiple myeloma) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). HSC-3 was cultured in DMEM medium (Nacalai Tesque, Inc., Kyoto, Japan), supplemented with 100 μg/mL streptomycin, 100 U/mL penicillin, and 0.25 μg/mL amphotericin B (Nacalai Tesque, Inc.), and 10% (v/v) heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The cell lines (MKN45, NUGC-4, PANC-1, CHO-K1, and P3U1) were cultured in RPMI-1640 medium (Nacalai Tesque, Inc.), supplemented as indicated above. All cells were cultured using a humidified incubator at 37 °C, in an atmosphere of 5% CO₂ and 95% air.