TFV reference standard was purchased from United States Pharmacopeia, (Rockville, MD). FTC, EVG and RPV reference standards were purchased from Sequoia, Pangbourne, United Kingdom. Labelled internal standards (TFV-d6, FTC-13C,15N2, EVG-d6 and RPV-d6) were purchased from Toronto Research Chemicals Inc., Canada. Milli-Q water was obtained from in-house Milli-Q water purification system, Millipore, USA. LC-MS grade methanol, acetonitrile, and formic acid were purchased from Fisher Scientific, USA. Whatman 903 protein saver DBS cards were purchased from GE Healthcare, Cardiff, UK.
Evg d6
Manufactured by LGC
Sourced in Canada
The EVG-d6 is a wafer bonding system designed for high-precision alignment and bonding of substrates. It features advanced alignment capabilities and supports a range of wafer sizes and bonding techniques.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Lc ms grade methanol, by Thermo Fisher Scientific (2 mentions)
Tfv d6, by LGC (2 mentions)
Milli q water, by Merck Group (2 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
3 protocols using evg d6
1
Quantification of HIV Antiretrovirals
2
Quantification of Antiretrovirals
3
Quantification of PrEP Drugs in Biological Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of EVG, TAF, and TFV were measured by HPLC—MS/MS as previously described [33]. Briefly, analytes were extracted from 0.1 mL of plasma, a single swab, or 0.25 mL of tissue homogenate by protein precipitation using 500 μL of methanol containing an internal standard [13C-labeled TFV, deuterium-labelled TAF (Moravek, Inc., Brea, CA), and deuterium-labelled EVG (EVG-d6) (Toronto Research Chemicals, Toronto, Canada)]. Drug concentrations measured in vaginal swabs were converted to ng/mL based on the net weight (assuming 1 μL = 1 mg). The lower limit of quantification (LLOQ) of the assays in plasma was 10 ng/mL for TFV and TAF and 5 ng/mL for EVG. The LLOQ of all 3 analytes in vaginal fluids was 10 ng/mL and 1 ng/mg in tissue homogenates. All calibration curves had r2 values greater than 0.99.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!