Borosilicate glass slides

Manufactured by Thermo Fisher Scientific

Sourced in United States

Borosilicate glass slides are a type of laboratory equipment used for various applications in scientific research and analysis. They are made of borosilicate glass, a durable and heat-resistant material that is commonly used in scientific applications. The slides provide a flat, smooth surface for mounting and analyzing samples under a microscope.

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Lab products found in correlation

3 4 ethylenedioxythiophene edot, by Merck Group (1 mentions) 3 glycidyloxypropyl trimethoxysilane gops, by Merck Group (1 mentions) Pedot, by Merck Group (1 mentions)

Close spelling variants of this product

Lab-Tek chambered borosilicate cover glass slides Borosilicate glass Glass slides

4 protocols using borosilicate glass slides

PEDOT:PSS Thin Film Fabrication

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PEDOT: PSS dispersion was purchased from Sigma-Aldrich (Product number: 483095, CAS number 155090-83-8, Sigma-Aldrich, MO, United States). The nominal solid content and the PEDOT to PSS ratio of the PEDOT: PSS dispersion were 1.3% and 1:1.6 by weight, respectively. (3-Glycidyloxypropyl) trimethoxysilane (GOPS, Sigma-Aldrich, MO, United States) was used as received without further purification. 3,4-Ethylenedioxythiophene (EDOT) and polysulfate sodium (NaPSS) were obtained from Sigma Aldrich (MO, United States) and used to obtain the solution (EDOT 0.01 M; NaPSS 0.8% w/w) for the electrochemical deposition of PEDOT: PSS. Borosilicate glass slides (thickness ∼1 mm, Thermo Scientific, MA, United States) were used as substrate for the deposition of the PEDOT: PSS films by spin coating after ultrasonic cleaning in a mixed solution of pure ethanol, isopropanol and milliQ water (1:1:1 by volume) and drying under sustained flux of pure nitrogen. Fluorine Tin Oxide (FTO) slabs (1.5 × 1 cm², thickness ∼1 mm) were used as conductive substrate for the electrochemical polymerisation of PEDOT:PSS films. FTO slabs were used after ultrasonic cleaning in a solution of pure ethanol and milliQ water (1:1), drying under sustained flux of pure nitrogen and a heating step at 450°C for 30 min in order to clean the surface from organic residues and after cooling overnight in the muffle.

Pisciotta A., Lunghi A., Bertani G., Di Tinco R., Bertoni L., Orlandi G., Biscarini F., Bianchi M, & Carnevale G. (2022). PEDOT: PSS promotes neurogenic commitment of neural crest-derived stem cells. Frontiers in Physiology, 13, 930804.

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PEDOT:Nafion Dispersion Coating Synthesis

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PEDOT:Nafion water dispersion was synthesized according to previously reported protocol [14 (link)]. The obtained water dispersion of PEDOT:Nafion (1 mL) was diluted with water (5 mL), and the mixture was sonicated at room temperature for 15 min. This treatment is necessary to reduce the aggregates in order to give an optimal deposition of PEDOT:Nafion. Subsequently, coatings were fabricated by drop casting 30 µL of the PEDOT:Nafion dispersion with a pipette on borosilicate glass slides (1 × 1 cm², thickness ~ 1 mm, average root mean square (RMS) roughness: (0.21 ± 0.02) nm, Thermo Fisher Scientific, Milan, Italy) previously cleaned with an isopropyl alcohol, ethanol and water (1:1:1 vol%) solution and dried with N₂. Subsequently, coatings were annealed at 120 °C for 40 min.

Guzzo S., Carli S., Pavan B., Lunghi A., Murgia M, & Bianchi M. (2021). Evaluation of the In Vitro Biocompatibility of PEDOT:Nafion Coatings. Nanomaterials, 11(8), 2022.

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Glass Surface Functionalization for Polymer Bonding

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To secure polymer bonding to glass, MAPTMS-treated glass slides were prepared according to a protocol adopted from Kapyla et al. [27 (link)]. Briefly, circular 12 mm diameter borosilicate glass slides (Thermo Fisher Scientific, Waltham, MA, USA) were washed in ethanol, then immersed in a mixture of MAPTMS/ethanol/acetic acid/water overnight and finally washed in ethanol in an ultrasonic bath (EMAG, Mörfelden-Walldorf, Germany).
The hybrid materials were then spin-coated @ 3000 rpm for 30 s per sample and left at room temperature in the dark overnight for the solvents to evaporate. The samples were then polymerised using a UV lamp (UV-C, G15W T8, Sylvania, London, UK) for at least 5 h at around 20 cm distance, corresponding to about 1.8 mW/cm². The samples were sterilised under UV for at least one hour on each side, subsequently washed in sterile PBS to remove residual initiator and low molecular weight components. They were then immersed in sterile growth medium prior to cell culture.

Balčiūnas E., Dreižė N., Grubliauskaitė M., Urnikytė S., Šimoliūnas E., Bukelskienė V., Valius M., Baldock S.J., Hardy J.G, & Baltriukienė D. (2019). Biocompatibility Investigation of Hybrid Organometallic Polymers for Sub-Micron 3D Printing via Laser Two-Photon Polymerisation. Materials, 12(23), 3932.

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Fabrication of Ultrathin Electrochemical Cells

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Borosilicate glass slides (Thermos Scientific) were cleaned by rinsing thoroughly with deionized water and isopropyl alcohol and dried under a flow of pure nitrogen. Chromium (chrome-plated tungsten rods, 99.999%, Kurt J. Lesker) and gold (Au pellets, 99.999%, Kurt J. Lesker) were deposited on glass using an in-house thermal evaporator. The rate of metal deposition and the film thickness were monitored by a quartz crystal microbalance. A 2 μm layer of photoresist (AZ 5214E) was spin-coated on the Au film and patterned using a simple photomask to open a small circular window (typical 20–30 μm in diameter) in the polymer. The exposed Au microdisk was first etched with gold etchant, and the Cr layer was further etched by applying a +1 V dc potential in a 50 mM phosphate buffer at pH 7.4. The etching processes were monitored under the optical microscope (Figure 1b-d) and terminated immediately when the desired UTLC size was reached. A drop of electrolyte solution was kept on the top of the UTLC to maintain the nanogap geometry before and during the CV tests (Figure 1a, after step v). The fabrication process is highly repeatable with a success rate greater than 90%. The cell–cell variation comes mainly from the depth in Au/Cr etching, which can only be monitored and manually controlled to a certain extent under an optical microscope.

Lu J, & Zhang B. (2017). Electrostatic Ion Enrichment in an Ultrathin-Layer Cell with a Critical Dimension between 5 and 20 nm. Analytical chemistry, 89(5), 2739-2746.

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