Log In Sign up
The largest database of trusted experimental protocols

Aria 1

Manufactured by Agilent Technologies
Visit Supplier
Sourced in United States

Aria 1.6 software is a data analysis and visualization tool developed by Agilent Technologies. The software is designed to process and analyze complex data sets generated by Agilent's analytical instruments, enabling users to streamline their data management and reporting workflows.

Automatically generated - may contain errors

Lab products found in correlation

Monarch total rna extraction kit, by New England Biolabs (2 mentions) Maximah rt, by New England Biolabs (2 mentions) Ariamx qpcr machine, by Agilent Technologies (2 mentions) Fitcanti hla dq, by BioLegend (2 mentions) Taqman master mix, by Thermo Fisher Scientific (2 mentions) Specific taqman probes, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Merck Group (1 mentions) Cfx 96 rt system c1000 thermal cycler, by Bio-Rad (1 mentions) Brilliant 3 ultra fast sybr green qpcr master mix, by Agilent Technologies (1 mentions) Primescript rt master mix, by Takara Bio (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Ariamx real time pcr system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Agilent Aria 1.6 software

5 protocols using aria 1

1

HLA-DQB1 Expression Analysis in Clones

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of HLA-DQB1 expression on HH WT (C/C) and
ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA
extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme
following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1
in 4 with HLA-DQB1 (Assay ID:Hs00409790) and
actinB (Assay ID:Hs01060665) probes and Taqman MasterMix
(Thermofisher). Samples were run on an ARIAmx qPCR machine (Agilent) and data
analyzed with Aria 1.5 (Agilent) software. Expression is represented as
2-delta(HLA-DQB1 Ct - ActinBCt). For analysis of protein expression, clones that survived after
3–4 months of culture were washed with PBS and stained with FITC
anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface
expression assessed by flow cytometry. Data was analyzed using Flowjo and
Graphpad PRISM.
Gutierrez-Arcelus M., Baglaenko Y., Arora J., Hannes S., Luo Y., Amariuta T., Teslovich N., Rao D.A., Ermann J., Jonsson A.H., Navarrete C., Rich S.S., Taylor K.D., Rotter J.I., Gregersen P.K., Esko T., Brenner M.B, & Raychaudhuri S. (2020). Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci. Nature genetics, 52(3), 247-253.
+ Open protocol
+ Expand
2

HLA-DQB1 Expression Analysis in Clones

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of HLA-DQB1 expression on HH WT (C/C) and
ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA
extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme
following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1
in 4 with HLA-DQB1 (Assay ID:Hs00409790) and
actinB (Assay ID:Hs01060665) probes and Taqman MasterMix
(Thermofisher). Samples were run on an ARIAmx qPCR machine (Agilent) and data
analyzed with Aria 1.5 (Agilent) software. Expression is represented as
2-delta(HLA-DQB1 Ct - ActinBCt). For analysis of protein expression, clones that survived after
3–4 months of culture were washed with PBS and stained with FITC
anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface
expression assessed by flow cytometry. Data was analyzed using Flowjo and
Graphpad PRISM.
Gutierrez-Arcelus M., Baglaenko Y., Arora J., Hannes S., Luo Y., Amariuta T., Teslovich N., Rao D.A., Ermann J., Jonsson A.H., Navarrete C., Rich S.S., Taylor K.D., Rotter J.I., Gregersen P.K., Esko T., Brenner M.B, & Raychaudhuri S. (2020). Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci. Nature genetics, 52(3), 247-253.
+ Open protocol
+ Expand
3

RNA Extraction and Real-Time PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from myometrial tissue using TRI-Reagent (Sigma Aldrich, Germany) following the standard protocol. The details of RNA extraction and preparation for further analyses were described previously [19 (link)]. RNA precipitates were used for analyses that were integral electrophoretically (separation in 1.5% agarose gel) and possessed an optical density ration A260/A280 in the range of 1.8–2.0 (spectrophotometer Tecan, Männedorf, Switzerland). The Real-Time PCR was performed according to the guidelines provided by Bustin et al. (2009) [58 (link)]. The amplification was conducted using 4 pg/μL aliquots of extracted RNA, TaqMan®RNA-to- 1-Step Kit (Applied Biosystems, Foster City, CA, USA) and specific TaqMan probes provided by Applied Biosystems (Foster City, CA, USA) listed in Table 4. Amplification was conducted in an AriaMX apparatus (Agilent Technologies, Santa Clara, CA, USA), with a standard thermal profile suggested by the producer. The Ct values were obtained with Aria 1.6 software (Agilent Technologies, Santa Clara, CA, USA) and used for cycle threshold (Ct) calculation. The Ct values of the tested genes were normalized with the geometrical mean of the reference genes and used for 2−∆∆Ct calculation [59 (link)].
Drzewiecka E.M., Kozlowska W., Zmijewska A., Wydorski P.J, & Franczak A. (2021). Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period. International Journal of Molecular Sciences, 22(6), 2920.
+ Open protocol
+ Expand
4

SARS-CoV-2 RNA Detection using Bio-T Kit

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Bio-T kit TriStar COVID-19 (Biosellal, France), which targets the envelope gene (E) of the Sarbecovirus, and RNA-dependent RNA polymerase (RdRp) in the Orf1ab gene, were used for SARS-CoV-2 RNA detection, according to the manufacturer’s instructions, using 5 μL of extracted RNA. The AriaMx Real-Time PCR Thermal Cycler System (Agilent Technologies, Santa Clara, CA, USA) was used for amplification. The conditions consisted of 1 cycle of 20 min at 50 °C, 1 min at 95 °C, followed by 40 cycles of 10 s at 95 °C, and 45 s at 60 °C. The results were analyzed using Agilent Aria 1.6 software, in which a cycle threshold value (Ct-value)  <  40 for all target genes was defined as a positive result.
Positive samples were confirmed in the virology unit of the University Hospital of Caen using SARS-CoV-2-standardized real-time multiplex one-step reverse-transcription PCR protocol targeting Orf1, S and N genes. RNA extracts were submitted to the 3-AllplexTM 2019-nCoV assay (Seegene, Seoul, South Korea), using the manufacturer’s instructions for the CFX-96 RT System C1000 Thermal Cycler (Biorad, Hercules, CA, USA), and the SARS-CoV-2 Viewer for Real-Time, instrument version 3.19.003.010 (Seegene, Seoul, Korea).
Krafft E., Denolly S., Boson B., Angelloz-Pessey S., Levaltier S., Nesi N., Corbet S., Leterrier B., Fritz M., Leroy E.M., Gouilh M.A., Cosset F.L., Kodjo A, & Legros V. (2021). Report of One-Year Prospective Surveillance of SARS-CoV-2 in Dogs and Cats in France with Various Exposure Risks: Confirmation of a Low Prevalence of Shedding, Detection and Complete Sequencing of an Alpha Variant in a Cat. Viruses, 13(9), 1759.
+ Open protocol
+ Expand
5

Real-Time qRT-PCR of ER Stress Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from the indicated cell lines using a Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA aliquots of 1 μg were then reverse transcribed using PrimeScript RT Master Mix (RR036A; Takara, Japan) according to standard protocols. For qRT-PCR, the reactions were performed using the AriaMx Real-Time PCR kit (Agilent Technologies, USA) following the manufacturer’s instructions. qRT-PCR was performed on cDNA samples using Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies), and the signal was detected by an AriaMx Real-time PCR System (Agilent Technologies). The fluorescence threshold value was calculated using Agilent Aria 1.6 software. The PCR primers used were as follows: EHMT1 (forward, 5’-CAGGACTTCCAAGGAGAGCA-3’ and reverse, 5’-ACTCAGGTCAGACTCGT CAC-3’), CHOP (forward, 5’-GGAAACAGAGTGGTCAT TCCC-3’ and reverse, 5’-CTGCTTGA GCCGTTCATTCTC-3’), GRP78 (forward, 5’-CA TCACGCCGTCCTATGTCG-3’ and reverse, 5’-CGTCAAAGACCGTGTTCTCG-3’), ATF4 (forward, 5’-ATGACCGAAATGAGCTT CCTG-3’ and reverse, 5’-GCTGGAGAA CCCATGAGGT-3’), and ACTB (forward, 5’-ACT CTTCCAGCCTTCCTTCC-3’ and reverse, 5’-CAATGCCAGGGTACATGGTG-3’).
Kim K., Ryu T.Y., Lee J., Son M.Y., Kim D.S., Kim S.K, & Cho H.S. (2022). Epigenetic Silencing of CHOP Expression by the Histone Methyltransferase EHMT1 Regulates Apoptosis in Colorectal Cancer Cells. Molecules and Cells, 45(9), 622-630.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!