Wp 250 sy spectrometer

All solvents were purified before use. N-bromosuccinimide, thiourea, 2-methylbenzyl chloride, 3-fluorobenzyl chloride, 2-chlorobenzyl chloride, 2,4-dichlorobenzyl chloride, 3,4-dichlorobenzyl chloride, 4-bromobenzyl chloride, 2-chloroacetamide, and ethyl chloroacetate were purchased from Acros Organics and used without purification. Compounds 2, 3, 4b, and 4h were prepared using methods described previously [10 ]. The reactions were monitored by thin-layer chromatography (TLC) using Fluka silica gel (60 F 254) plates (0.25 mm). Visualization was made with UV light. The melting points of the synthesized compounds were taken on a melting point tube. Infrared spectra were recorded on a Bruker Tensor 37 spectrometer (Germany). The ¹H-NMR spectra were recorded on a Bruker WP 250 SY spectrometer (250.13 MHz) (Germany). Chemical shifts are reported relative to chloroform (δ=7.25 ppm) for ¹H-NMR. The mass spectra were recorded on an Agilent LC/MSD SL 1100 instrument (USA).

${}^1$ H NMR spectra were recorded on a Bruker WP250 SY spectrometer (Bremen, Germany) and a Bruker Avance AV300 spectrometer (Bremen, Germany) using tetramethylsilane as an internal standard.
Size-exclusion chromatography (SEC) analysis was carried on a chromatographic system consisting of a Staier seriya 2 high-pressure pump (Akvilon, Podolsk, Russia), a Smartline RI 2300 refractometric detector, and a Jet stream 2 Plus thermostat (Knauer, Berlin, Germany). The temperature was $+40\pm 0.1{}^{\circ }\mathrm{C}$ , THF or toluene + 2% THF were used as eluents, and the flow rate was $1.0\mathrm{mL}{\min }^{-1}$ . The setup consisted of a column $300\times 7.8$ mm packed with a Phenogel sorbent (Phenomenex, Torrance, CA, USA) with a particle size of 5 mm and pore size in the range from ${10}^3$ to ${10}^5$ Å. Calibration with linear polystyrene standards was used to estimate the molecular weight.
Transmission electron microscopy (TEM) measurements were carried out on a LEO912 AB OMEGA microscope (LEO Elektronenmikroskopie GmbH, Oberkochen, Germany) operating at accelerating voltages of 60, 80, 100, 120 kV.