Anti human cd8 monoclonal antibody

Manufactured by BD

Sourced in Japan, United States

The Anti-human CD8 monoclonal antibody is a laboratory reagent used to identify and quantify CD8-positive cells in biological samples. It is a specific and sensitive tool for the detection and analysis of T-lymphocytes expressing the CD8 surface marker.

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Lab products found in correlation

Macsquant device, by Miltenyi Biotec (1 mentions)

2 protocols using anti human cd8 monoclonal antibody

Identifying Antigen-Specific CTLs using HLA Tetramers

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Cells were incubated with a phycoerythrin (PE)-labelled HLA-A*02:01 PSF1_79–87 peptide-binding HLA tetramer (PSF1 tetramer) (Medical & Biological Laboratories, Nagoya, Japan) and then stained with an allophycocyanin (APC)-labelled anti-human CD8 monoclonal antibody (BD Biosciences). The cells were analysed by a MACSQuant device (Miltenyi Biotec). HLA-A*02:01-restricted PSF1_79–87 CTLs were defined as CD8⁺/PSF1-tetramer⁺ T cells^{48 (link)}.

Yoshida M., Ishioka Y., Ozawa T., Okuyama H., Iguchi M., Ota T., Ito T., Nagira M., Morita A., Tanaka H., Naito H., Kidoya H, & Takakura N. (2017). Soluble HLA-associated peptide from PSF1 has a cancer vaccine potency. Scientific Reports, 7, 11137.

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Immunohistochemical Staining of PD-L1 and CD8+ T-Cells

Cited 1 time

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Immunohistochemical (IHC) staining was performed as described previously.18 (link) Briefly, following deparaffinization, rehydrating and antigen retrieval, primary antibodies were applied to slides, incubated at 4°C overnight, followed by incubation with secondary antibody (Dako Denmark A/S, Glostrup, Denmark) at 37°C for 30 minutes. A rabbit anti-human PD-L1 monoclonal antibody (1:50 dilution, SP142; Spring Bioscience, Inc., CA, USA) and antihuman CD8 monoclonal antibody (1:50 dilution; BD Bioscience, Franklin Lakes, NJ USA) were used as specific markers for PD-L1 and CD8⁺ T-cells, respectively. Staining was carried out with DAB and counterstaining was performed with hematoxylin.

Zhu Y., Wang X.Y., Zhang Y., Xu D., Dong J., Zhang Z., Yi C.H., Jia H.L, & Yang X. (2018). Programmed death ligand 1 expression in human intrahepatic cholangiocarcinoma and its association with prognosis and CD8+ T-cell immune responses. Cancer Management and Research, 10, 4113-4123.

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