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Anti pmypt1 t696

Manufactured by Merck Group
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Anti-pMYPT1 (T696) is a laboratory reagent used in research applications. It is an antibody that specifically binds to the phosphorylated form of the MYPT1 protein at the threonine 696 residue. This antibody can be used to detect and quantify the phosphorylation status of MYPT1 in biological samples.

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Lab products found in correlation

Anti tubulin, by Cell Signaling Technology (2 mentions) Peroxidase conjugated anti mouse igg, by Cell Signaling Technology (2 mentions) Anti pmlc2 s18t19, by Cell Signaling Technology (2 mentions) Recombinant mypt1, by Merck Group (1 mentions)

3 protocols using anti pmypt1 t696

1

Immunoblot Analysis of Myosin Phosphorylation

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Cells were seeded onto CellStar tissue culture plates and grown to confluence in Ham’s F-12 media supplemented with L-glutamine and 10% FBS at 37°C in 5% CO2. Cells were starved in serum-free media for 72 hours at 37°C in 5% CO2, then treated with vehicle, Af extract, or purified Alp 1 diluted in serum-free medium for 24 hours. Lysates were prepared in RIPA buffer and immunoblotted using the following primary antibodies: anti-pMYPT1 (T696) (Millipore), anti-pMLC2 (S18T19) (Cell Signaling Technologies) and anti-tubulin (Cell Signaling Technologies The following secondary antibodies were used: donkey anti-mouse (Jackson ImmunoResearch Laboratories, peroxidase-conjugated anti-mouse IgG or donkey anti-rabbit (Cell Signaling Technologies). Immunoblots were visualized using standard chemiluminescence protocols.
Balenga N.A., Klichinsky M., Xie Z., Chan E.C., Zhao M., Jude J., Laviolette M., Panettieri RA J.r, & Druey K.M. (2015). A fungal protease allergen provokes airway hyperresponsiveness in asthma. Nature communications, 6, 6763.
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2

Rho-Kinase Activity Quantification

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The Rho-kinase activity was evaluated by measuring its substrate phosphorylation (pMYPT1) using 100 µg of muscle lysate, 1 mM of ATP, and 500 ng of recombinant MYPT1 (Millipore®). This mixture was incubated for 30 min under agitation at 30°C. The reaction was stopped using 2x Laemmli and submitted to IP protocol. The membrane was incubated using anti-pMYPT1 (T696) from Millipore®.
Muñoz V.R., Gaspar R.C., Severino M.B., Macêdo A.P., Simabuco F.M., Ropelle E.R., Cintra D.E., da Silva A.S., Kim Y.B, & Pauli J.R. (2021). Exercise Counterbalances Rho/ROCK2 Signaling Impairment in the Skeletal Muscle and Ameliorates Insulin Sensitivity in Obese Mice. Frontiers in Immunology, 12, 702025.
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3

Immunoblot Analysis of Myosin Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded onto CellStar tissue culture plates and grown to confluence in Ham’s F-12 media supplemented with L-glutamine and 10% FBS at 37°C in 5% CO2. Cells were starved in serum-free media for 72 hours at 37°C in 5% CO2, then treated with vehicle, Af extract, or purified Alp 1 diluted in serum-free medium for 24 hours. Lysates were prepared in RIPA buffer and immunoblotted using the following primary antibodies: anti-pMYPT1 (T696) (Millipore), anti-pMLC2 (S18T19) (Cell Signaling Technologies) and anti-tubulin (Cell Signaling Technologies The following secondary antibodies were used: donkey anti-mouse (Jackson ImmunoResearch Laboratories, peroxidase-conjugated anti-mouse IgG or donkey anti-rabbit (Cell Signaling Technologies). Immunoblots were visualized using standard chemiluminescence protocols.
Balenga N.A., Klichinsky M., Xie Z., Chan E.C., Zhao M., Jude J., Laviolette M., Panettieri RA J.r, & Druey K.M. (2015). A fungal protease allergen provokes airway hyperresponsiveness in asthma. Nature communications, 6, 6763.
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