All restriction enzymes and DNA ladders were acquired from New England Biolabs (NEB) (MA, USA). The expression vector pET30a was obtained from Novagen (Germany). Ni2+-NTA (nitrilotriacetic acid) agarose beads were obtained from Qiagen (USA). Hygromycin, paromomycin and zeocin were acquired from Sigma Aldrich (Germany). Protein ladders were obtained from Fermentas. Borrelidin was purchased from Abcam (Cambridge, UK). The in vitro tRNA transcription kit was obtained from Invitrogen (CA, USA). The anti-LdThrRS antibody was commercially synthesized in rabbits by Merck (Germany).
Borrelidin
Manufactured by Abcam
Sourced in United Kingdom
Borrelidin is a laboratory reagent. It is a natural product isolated from the bacterium Streptomyces saturatus. Borrelidin is an inhibitor of the enzyme threonyl-tRNA synthetase.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Hygromycin, by Merck Group (1 mentions)
Zeocin, by Merck Group (1 mentions)
Paromomycin, by Merck Group (1 mentions)
Dna ladder, by New England Biolabs (1 mentions)
Protein ladder, by Thermo Fisher Scientific (1 mentions)
Agarose beads, by Qiagen (1 mentions)
Ni2 nta, by Qiagen (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Anti puromycin, by Merck Group (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Anti muc1, by Santa Cruz Biotechnology (1 mentions)
Anti c myc, by Santa Cruz Biotechnology (1 mentions)
Anti muc1, by Abcam (1 mentions)
2 protocols using borrelidin
1
Purification of LdThrRS Enzyme
2
Protein Expression and Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-MUC1 (cat# ab109185) was purchased from Abcam (Cambridge, UK), Anti-MUC1 (cat# sc-7313), anti-ThrRS (cat# sc-166146), anti-c-Myc (cat# sc-40), and anti-AlaRS (cat# sc-98547) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), anti-alpha-tubulin (cat# T6074) was purchased from Sigma-Aldrich (St Louis, MO, USA), and anti-puromycin (cat# MABE343) was purchased from Millipore (Billerica, MA, USA). L-[35S]-Methionine (Met) (cat# NEG709A) was purchased from PerkinElmer (Waltham, MA, USA). Threonine, L-[3-3H] (cat# ART0330) was purchased from American Radiolabeled Chemicals (Saint Louis, MO, USA). Borrelidin (cat# ab144212) was purchased from Abcam. 5′-O-(N-(L -threonyl)-sulfamoyl) adenosine was purchased from Integrated DNA Technologies (Coralville, IA, USA). Lipofectamine 2000 (cat# 11668030) and puromycin (cat# A1113802) were purchased from Thermo Fisher (Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!