The random fruit samples of 5 kg were collected from each of the 4 organic and 4 conventional plots and transported to the laboratory of the Department of Functional and Organic Food. Fruits were washed and cut into cubes (5 × 5 × 5 mm). The 10–15 g fresh fruit samples were used for dry matter content analysis. The 100 g samples were frozen in −80 °C for 24 h and freeze-dried using Labcono 2.5 freeze-dryer (Labconco Corporation, Kansas City, MO, USA), under the temperature of −40 °C and the pressure of 0.100 mBa. Dried samples were ground in laboratory mill and stored in scintillation vials in −80 °C temperature before further analyses of polyphenols (phenolic acids and flavonoids), carotenoids (lutein, zeaxanthin, β-carotene), vitamin C (dehydroascorbic acid, l -ascorbic acid), and chlorophylls (a, b) content.
Labcono 2.5 freeze dryer
Manufactured by Labconco
Sourced in United States
The Labconco 2.5 freeze-dryer is a compact laboratory instrument designed for lyophilization, a process that removes water from a sample through sublimation. It features a 2.5 square foot condenser capacity and can accommodate a variety of sample sizes and types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using labcono 2.5 freeze dryer
1
Detailed Phytochemical Analysis of Organic and Conventional Fruits
2
Chemical Composition Analysis of Fresh Fruits
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The random fresh fruits samples from each experimental plot, from two harvests in each season (6 August and 5 September 2012; 27 August and 4 October 2013), were transported to the Department of Functional and Organic Food, Warsaw University of Life Sciences (Warsaw, Poland) for the dry matter and chemical composition analyses. Fruits were washed and cut into cubes (5 × 5 × 5 mm). Fresh fruit samples were used for dry matter content analysis. At the same time, 100 g samples were frozen (-80 • C for 24 h) and subjected to a freeze-drying process using a Labcono 2.5 freeze-dryer (Labconco Corporation, Kansas City, MO, USA). The freeze-drying process was carried out at -40 • C and the pressure of 0.100 mBa. After freeze-drying, the samples were ground in a laboratory mill A-11 (IKA ® -Werke GmbH & Co. KG, Staufen im Breisgau, Germany) and stored in scintillation vials at -80 • C until further analyses of carotenoids, chlorophylls, nitrates and nitrites content.
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